干旱胁迫对2种柑橘幼苗生长形态、渗透调节物质含量和抗氧化酶活性的影响

doi:10.3969/j.issn.1004-1524.2017.09.13

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (9): 1515-1523.DOI: 10.3969/j.issn.1004-1524.2017.09.13

干旱胁迫对2种柑橘幼苗生长形态、渗透调节物质含量和抗氧化酶活性的影响

冯芳芳, 魏清江, 苏受婷, 宁少君, 廖小娜, 辜青青^*

江西农业大学农学院,江西南昌330045

收稿日期:2017-03-23 出版日期:2017-09-20 发布日期:2017-09-27
通讯作者: 辜青青,E-mail:qingqinggu2006@126.com
作者简介:冯芳芳(1989—),女,河南濮阳人,硕士研究生,从事果树逆境生物学研究。E-mail:1454140420@qq.com
基金资助:
国家自然科学基金项目(31460496); 江西省青年科学基金项目(20161BAB214175)

Effect of drought on growth morphology, osmolyte content and antioxidant enzyme activity of two citrus seedlings

FENG Fangfang, WEI Qingjiang, SU Shouting, NING Shaojun, LIAO Xiaona, GU Qingqing^*

College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China

Received:2017-03-23 Online:2017-09-20 Published:2017-09-27

摘要/Abstract

摘要： 采用盆栽控水的方法,比较干旱胁迫对2种抗旱性不同的柑橘——三湖红橘和三湖化红生长形态、渗透调节物质含量和抗氧化酶活性的影响。结果表明:干旱胁迫可以降低2种柑橘的株高,增加根冠比,同时降低三湖化红主根长,增加三湖红橘主根长;此外,干旱胁迫导致2种柑橘幼苗叶片和根系的脯氨酸含量显著增加,三湖红橘叶片脯氨酸含量增幅更大;除三湖红橘叶片的可溶性糖含量降低外,干旱胁迫后2种柑橘的可溶性糖和可溶性蛋白含量均升高。酶活性分析表明,干旱胁迫后2种柑橘叶片中SOD和CAT活性均下降,而根中2种酶活性均上升;三湖红橘叶片POD活性先升高后降低,根中POD活性显著升高;三湖化红叶片POD活性一直显著高于对照,根中POD活性则显著降低。综上,三湖红橘的抗旱性强于三湖化红可能与其在干旱胁迫条件下主根长度增加,根冠比增大从而提高根系水分吸收能力有关。此外,干旱胁迫后渗透物质尤其是叶片脯氨酸的积累和POD活性的升高,也有助于其应对干旱胁迫带来的伤害。

关键词: 柑橘幼苗, 干旱胁迫, 生长形态, 渗透调节物质, 抗氧化酶

Abstract: Pot experiments were conducted to compare the effects of drought on growth morphology, osmolyte content, and antioxidant enzyme activity of two citrus seedlings Sanhuhongju and Sanhuhuahong. Results showed that, plant heights were decreased, while root-shoot ratios were increased in both seedlings under drought. The taproot length was decreased in Sanhuhuahong while it was increased in Sanhuhongju under drought. Moreover, proline content was significantly increased in leaves and roots of the two citrus seedlings, and greater increase was found in leaves of Sanhuhongju than that of Sanhuhuahong. Except for soluble sugar in leaves of Sanhuhongju, contents of soluble sugar and soluble protein were increased in the treated seedlings. Enzyme analysis revealed that the activities of superoxide dismutase (SOD) and catalase (CAT) were decreased in leaves whereas increased in roots of both seedlings. The leaf peroxydase (POD) activity was firstly increased and then decreased in Sanhuhongju, and it was always increased in Sanhuhuahong. In roots, the POD activity was significantly increased in Sanhuhongju and was significantly decreased in Sanhuhuahong under drought. Overall, the higher drought tolerance shown by Sanhuhongju might be related to the longer taproot and higher root-top ratios, which could improve the water uptake ability of root. Additionally, the accumulation of osmolytes especially the proline and the increase of POD activities in roots might also help the Sanhuhongju to cope with drought stress.

Key words: citrus seedling, drought stress, growth morphology, osmolyte, antioxidant enzyme

中图分类号:

S666.2

冯芳芳, 魏清江, 苏受婷, 宁少君, 廖小娜, 辜青青. 干旱胁迫对2种柑橘幼苗生长形态、渗透调节物质含量和抗氧化酶活性的影响[J]. 浙江农业学报, 2017, 29(9): 1515-1523.

FENG Fangfang, WEI Qingjiang, SU Shouting, NING Shaojun, LIAO Xiaona, GU Qingqing. Effect of drought on growth morphology, osmolyte content and antioxidant enzyme activity of two citrus seedlings[J]. , 2017, 29(9): 1515-1523.

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干旱胁迫对2种柑橘幼苗生长形态、渗透调节物质含量和抗氧化酶活性的影响

Effect of drought on growth morphology, osmolyte content and antioxidant enzyme activity of two citrus seedlings

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