家禽屠宰场环境和屠宰器械表面微生物菌群结构和耐药基因分析

doi:10.3969/j.issn.1004-1524.2018.07.20

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (7): 1249-1258.DOI: 10.3969/j.issn.1004-1524.2018.07.20

家禽屠宰场环境和屠宰器械表面微生物菌群结构和耐药基因分析

王佩佩^{1, 2, 3}, 杨华^{2, 3}, 戴贤君¹, 桂国弘^{2, 3}, 肖英平^{2, 3, *}

1.中国计量大学生命科学学院,浙江杭州 310018;
2.浙江省农业科学院农产品质量标准研究所,浙江杭州 310021;
3.浙江省植物有害生物防控省部共建国家重点实验室培育基地,浙江杭州 310021

收稿日期:2017-11-07 出版日期:2018-07-20 发布日期:2018-08-02
通讯作者: 肖英平,E-mail: ypxiaozju@126.com
作者简介:王佩佩(1991—),女,浙江绍兴人,硕士研究生,研究方向为食品微生物。E-mail: 244960537@qq.com
基金资助:
浙江省公益技术应用研究(2016C32073); 杭州市科技局社会发展项目(20150533B77); 浙江省植物有害生物防控重点实验室—省部共建国家重点实验室培育基地项目(2010DS700124-ZM1608)

Microbial community structure and drug resistance gene on water, ground and surface of slaughtering equipment in poultry slaughterhouse

WANG Peipei^{1, 2, 3}, YANG Hua^{2, 3}, DAI Xianjun¹, GUI Guohong^{2, 3}, XIAO Yingping^{2, 3, *}

1. College of Life Science, China Jiliang University, Hangzhou 310018, China;
2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2017-11-07 Online:2018-07-20 Published:2018-08-02

摘要/Abstract

摘要： 为分析家禽屠宰场存在的水体、地面和与鸡肉接触的屠宰器械表面的菌群结构及其耐药基因携带状况,在浙江省某大型屠宰场的挂禽间、宰杀沥血间、浸烫间、脱毛间、净膛间区域取存在水体,用无菌纱布擦拭各区域的地面和器械表面采集微生物,采用基于16S rRNA基因V3-V4的Illumina 高通量测序方法分析其菌群结构多样性,并用PCR对细菌的9大类24种耐药基因进行检测分析。结果表明,屠宰场不同区域水体、地面和屠宰器械表面微生物均以变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria)为主,其相对丰度分别为27.55%~88.99%、3.45%~59.78%和4.35%~31.78%;优势菌属为不动杆菌属(Acinetobacter)、嗜冷杆菌属(Psychrobacter)、栖热菌属(Thermus)、假单胞菌属(Pseudomonas)和希瓦氏菌属(Shewanella),约占总类群46%。共检测到21种耐药基因分布在这些样品中,其中sulⅠ、sulⅡ和qnrS的检出率为100%,blaTEM、tetB和aadA1的检出率为94.4%,floR和mecC的检出率为88.9%。由此反映出屠宰场不同区域的水体、地面和屠宰器械表面存在一些条件性致病菌和腐败菌,且携带的耐药基因种类较多。

关键词: 家禽屠宰场, 菌群结构, 耐药基因, 高通量测序

Abstract: The aim of this study is to investigate the microbial community structure and resistance genes on water, surface of floor and slaughtering equipment. The water and the bacteria collected on floor and surface of slaughtering equipment by sterile gauze swab were sampled in hanging, slaughtering, scalding, dehairing, eviscerating rooms of a large-scale poultry slaughterhouse in Zhejiang Province. The microbial community structure was analyzed based on 16S rRNA V3-V4 by Illumina Hiseq 2500, while the nine major categories of 24 resistance genes were tested by PCR method. The results showed that Proteobacteria, Firmicutes and Actinobacteria were the predominant phyla on water and the surface of floor and slaughtering equipment in the different areas of the slaughterhouse, their relative abundance were 27.55%-88.99%, 3.45%-59.78%, 4.35%-31.78%, respectively. The dominant genera included Acinetobacter, Psychrobacter, Thermus, Pseudomonas and Shewanella, accounting for 46% of the total taxa. The drug resistance gene detection rate of sulⅠ, sulⅡ and qnrS was 100%, while blaTEM, tetB and aadA1 was 94.4%, and floR and mecC was 88.9%. It was concluded that there were some conditional pathogenic bacteria and spoilage bacteria with many kinds of bacterial resistance genes on water and the surface of ground and slaughtering equipments in different areas of the slaughterhouse.

Key words: poultry slaughterhouse, microorganisms, drug resistance gene, high throughput sequencing

中图分类号:

S851.4⁺3

王佩佩, 杨华, 戴贤君, 桂国弘, 肖英平. 家禽屠宰场环境和屠宰器械表面微生物菌群结构和耐药基因分析[J]. 浙江农业学报, 2018, 30(7): 1249-1258.

WANG Peipei, YANG Hua, DAI Xianjun, GUI Guohong, XIAO Yingping. Microbial community structure and drug resistance gene on water, ground and surface of slaughtering equipment in poultry slaughterhouse[J]. , 2018, 30(7): 1249-1258.

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家禽屠宰场环境和屠宰器械表面微生物菌群结构和耐药基因分析

Microbial community structure and drug resistance gene on water, ground and surface of slaughtering equipment in poultry slaughterhouse

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