泌乳初期奶牛相关脂肪因子及生理生化指标与脂肪肝的相关性

doi:10.3969/j.issn.1004-1524.2019.05.07

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (5): 722-729.DOI: 10.3969/j.issn.1004-1524.2019.05.07

泌乳初期奶牛相关脂肪因子及生理生化指标与脂肪肝的相关性

朱颍琨, 肖劲邦, 钱柏霖, 姜思汛, 尤留超, 张钺, 刘红, 马莉, 曹随忠, 余树民, 沈留红^*

四川农业大学动物医学院,动物疫病与人类健康四川省重点实验室/奶牛疾病研究中心,四川成都 611130

收稿日期:2018-09-11 出版日期:2019-05-25 发布日期:2019-05-23
通讯作者: *沈留红,E-mail: shenlh@sicau.edu.cn
作者简介:朱颍琨(1979—),男,安徽阜阳人,硕士研究生,从事反刍动物疾病及繁殖新技术研究。E-mail: CrhistianZHu@foxmail.com
基金资助:
国家重点研发计划(2018YFD0500900);四川省科技厅科技创新苗子工程(2017086);四川农业大学学科建设双支计划(03572174);四川农业大学本科生科研兴趣培养项目(2019185,2019186)

Correlations between adipokine, biochemical indicators in early lactation cows with fatty liver

ZHU Yingkun, XIAO Jinbang, QIAN Bolin, JIANG Sixun, YOU Liuchao, ZHANG Yue, LIU Hong, MA Li, CAO Suizhong, YU Shumin, SHEN Liuhong^*

College of Veterinary Medicine, Sichuan Agricultural University, Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, Chengdu 611130, China

Received:2018-09-11 Online:2019-05-25 Published:2019-05-23

摘要/Abstract

摘要： 为探究泌乳初期奶牛血清脂肪因子及生理生化指标与脂肪肝的相关性,选取四川某规模化奶牛场1~4胎围产期奶牛64头,分别于产后第7、14天早饲前尾静脉采血,检测产后第14天奶牛血清谷草转氨酶、血糖、游离脂肪酸水平,根据脂肪肝判定公式判定是否发生脂肪肝,检测并分析血清脂肪因子及生理生化指标与肝功能指标和能量代谢指标之间的相关性。结果显示,脂肪肝奶牛血清瘦素、脂联素水平显著低于健康奶牛(P<0.05),TNF-α水平显著高于健康奶牛(P<0.05),IL-6水平差异不显著(P>0.05)。瘦素水平与血糖、白蛋白呈显著正相关(P<0.05),与游离脂肪酸、β-羟基丁酸、谷草转氨酶、总胆红素、直接胆红素、总蛋白呈显著负相关(P<0.05),提示泌乳初期奶牛能量负平衡是脂肪肝发生的重要原因。脂联素水平与游离脂肪酸、β-羟基丁酸、谷草转氨酶呈显著负相关(P<0.05),与总蛋白、白蛋白呈显著正相关(P<0.05),提示泌乳初期脂肪肝奶牛存在着明显的肝功能损伤与脂质代谢紊乱。TNF-α水平与总胆红素、直接胆红素、谷丙转氨酶呈显著正相关(P<0.05),与血糖、白蛋白呈显著负相关(P<0.05);IL-6水平与谷丙转氨酶呈显著正相关(P<0.05),与总蛋白、白蛋白呈显著负相关(P<0.05),提示泌乳初期脂肪肝奶牛存在着明显的炎性反应。血清瘦素与脂联素水平呈显著正相关(P<0.05),与TNF-α呈显著负相关(P<0.05);TNF-α与脂联素呈显著负相关(P<0.05),与IL-6呈显著正相关(P<0.05),提示泌乳初期脂肪肝奶牛存在着糖脂代谢调控紊乱。综上所述,泌乳初期奶牛能量负平衡导致体脂动员,使得奶牛血清脂联素和瘦素水平下降,造成大量脂质合成于肝脏,肝脏功能受损,诱发炎性反应,TNF-α与IL-6水平随之上升,进而导致奶牛脂肪肝的发生,脂联素、瘦素和TNF-α可作为预测产后奶牛脂肪肝发生的指标。

关键词: 奶牛, 脂肪肝, 脂肪因子

Abstract: To explore correlations between adipokine, biochemical indicators in early lactation cows with fatty liver, 64 perinatal Chinese Holstein cows from a farm in Sichuan province were used in the present study. Tail vein blood was collected before morning feeding on 7 and 14 days after delivery, serum AST, Glu and NEFA levels of 14 days after delivery were tested. According to fatty liver determination formula, cows were grouped into fatty liver group and control group. Some adipokine and biochemical indicators were tested and their correlations with liver function indexes and energy metabolism indexes were analyzed. The results showed that leptin, adiponectin level of fatty liver early lactation cows were significantly lower than the normal group(P<0.05). TNF-α level of fatty liver early lactation cows was significantly higher than the normal group(P<0.05), IL-6 level appeared no significant difference between fatty liver cows and normal cows(P>0.05). Leptin had significant positive correlations with Glu and ALB levels(P<0.05), significant negative correlations with AST, TBIL, DBIL, TP, NEFA and BHBA levels(P<0.05), indicated that negative energy balance was an important factor of fatty liver. Adiponectin level had significant negative correlations with NEFA, BHBA and AST(P<0.05), significant positive correlations between TP and ALB(P<0.05), speculating liver function and energy metabolism disorders were occurred in fatty liver early lactation cows. TNF-α level had significant positive correlations with TBIL,DBIL and ALT levels(P<0.05), significant negative correlations with Glu and ALB(P<0.05). IL-6 level had a significant positive correlation with ALT level(P<0.05), significant negative correlations with TP and ALB levels(P<0.05), showed that obvious inflammatory stress was appeared in fatty liver cows. Leptin levels showed a significant positive correlation with adiponectin level(P<0.05), significant negative correlation with IL-6 levels, speculating metabolism regulation disorder in fatty liver cows. In summary, the negative energy balance of early lactation cows induced body fat mobilization and downregulated adiponectin and leptin levels, causing lipids accumulate in liver and damaged liver function and triggered inflammatory stress, rising IL-6 and TNF-α levels and induced fatty liver, adiponectin. Thus, leptin and TNF-α levels could be able to be a predicative index of fatty liver in early lactation cows.

Key words: cows, fatty liver, adipokines

中图分类号:

S858.23

朱颍琨, 肖劲邦, 钱柏霖, 姜思汛, 尤留超, 张钺, 刘红, 马莉, 曹随忠, 余树民, 沈留红. 泌乳初期奶牛相关脂肪因子及生理生化指标与脂肪肝的相关性[J]. 浙江农业学报, 2019, 31(5): 722-729.

ZHU Yingkun, XIAO Jinbang, QIAN Bolin, JIANG Sixun, YOU Liuchao, ZHANG Yue, LIU Hong, MA Li, CAO Suizhong, YU Shumin, SHEN Liuhong. Correlations between adipokine, biochemical indicators in early lactation cows with fatty liver[J]. , 2019, 31(5): 722-729.

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泌乳初期奶牛相关脂肪因子及生理生化指标与脂肪肝的相关性

Correlations between adipokine, biochemical indicators in early lactation cows with fatty liver

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