小麦黄花叶病毒P3蛋白致病功能域的鉴定和分析

doi:10.3969/j.issn.1004-1524.2019.05.13

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (5): 777-783.DOI: 10.3969/j.issn.1004-1524.2019.05.13

小麦黄花叶病毒P3蛋白致病功能域的鉴定和分析

张岩^1,2, 亓玉华^1,2, 鲁燕华², 杨乾坤², 何雨娟², 李俊敏^2,3, 陈剑平^1,2,3,*

1.福建农林大学植物保护学院,福建福州 350002;
2.浙江省植物有害生物防控国家重点实验室培育基地,农业农村部/浙江省植保生物技术重点实验室,浙江省农业科学院病毒学与生物技术研究所,浙江杭州 310021;
3.宁波大学植物病毒学研究所,浙江宁波 315211

收稿日期:2019-01-25 出版日期:2019-05-25 发布日期:2019-05-23
通讯作者: *陈剑平,E-mail: jpchen2001@126.com
作者简介:张岩(1993—),女,山东枣庄人,硕士研究生,主要从事植物病毒-宿主互作研究。E-mail: zhangxiaoli1028@163.com
基金资助:
国家现代农业小麦产业技术体系(CARS-3-1);农业农村部/浙江省植保生物技术重点实验室开放课题

Identification and analysis on pathogenicity-related domain of P3 protein of wheat yellow mosaic virus

ZHANG Yan^1,2, QI Yuhua^1,2, LU Yanhua², YANG Qiankun², HE Yujuan², LI Junmin^2,3, CHEN Jianping^1,2,3,*

1. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Biotechnology in Plant Protection of Zhejiang Province/Ministry of Agriculture and Rural Affairs, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Institute of Plant Virology, Ningbo University, Ningbo 315211, China

Received:2019-01-25 Online:2019-05-25 Published:2019-05-23

摘要/Abstract

摘要： 小麦黄花叶病毒(Wheat yellow mosaic virus,WYMV)隶属于马铃薯Y病毒科(Potyviridae)大麦黄花叶病毒属(Bymovirus),其基因组由两条正义单链RNA组成,共编码10个蛋白。先前的研究表明,马铃薯Y病毒组多种病毒编码的保守蛋白P3具有多种功能,在病毒复制、致病性、克服宿主抗性、细胞间移动等方面均具有重要作用,而P3在大麦黄花叶病毒属中是否有类似功能目前还未见报道。本研究利用烟草脆裂病毒(Tobacco rattle virus,TRV)介导的本氏烟基因沉默系统,明确了WYMV的P3碳端在本氏烟上具有致病功能域P3-C,但完整的P3则不具有致病能力。进一步移码突变研究表明,P3-C的致病性是由其编码的多肽引起的,而非病毒来源的小干扰RNA介导的宿主基因沉默。同时P3-C的两个跨膜结构域对致病性具有重要作用,单独表达任何一个跨膜结构域P3-C均不能在本氏烟上引起明显症状。

关键词: 小麦黄花叶病毒, P3蛋白, 致病性

Abstract: Wheat yellow mosaic virus (WYMV) belongs to the genus Bymovirus, family Potyviridae. Its genome consists of two sense single-stranded RNAs encoding a total of 10 proteins. Previous studies had shown that the conserved protein P3 encoded by potyviruses had multiple functions and played an important role in viral replication, pathogenicity, overcoming host resistance, and cell-to-cell movement. However, whether P3 had similar functions in bymovirus had never been reported yet. In this study, tobacco rattle virus(TRV)-mediated gene silencing system in Nicotiana benthamiana was used to study the function of WYMV P3. Our results indicated that C terminal of WYMV P3 (P3-C), but not the whole P3, had a pathogenic-related functional domain in N. benthamiana. Further frame shift mutation confirmed that, rather than the viral small interfering RNA-mediated host gene silencing, the pathogenicity was caused by the polypeptide of P3-C. In addition, the pathogenicity on N. benthamiana disappeared when any of the two predicted transmembrane domain in P3-C were mutated, indicating the important roles of the transmembrane domains in P3-C pathogenicity.

Key words: wheat yellow mosaic virus(WYMV), protein P3, pathogenicity

中图分类号:

S435.12

张岩, 亓玉华, 鲁燕华, 杨乾坤, 何雨娟, 李俊敏, 陈剑平. 小麦黄花叶病毒P3蛋白致病功能域的鉴定和分析[J]. 浙江农业学报, 2019, 31(5): 777-783.

ZHANG Yan, QI Yuhua, LU Yanhua, YANG Qiankun, HE Yujuan, LI Junmin, CHEN Jianping. Identification and analysis on pathogenicity-related domain of P3 protein of wheat yellow mosaic virus[J]. , 2019, 31(5): 777-783.

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小麦黄花叶病毒P3蛋白致病功能域的鉴定和分析

Identification and analysis on pathogenicity-related domain of P3 protein of wheat yellow mosaic virus

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