浙江农业学报 ›› 2020, Vol. 32 ›› Issue (5): 824-830.DOI: 10.3969/j.issn.1004-1524.2020.05.10

• 园艺科学 • 上一篇    下一篇

杧果品种亲缘关系的CDDP分析

张宇, 张继, 荣涛, 欧克纬*, 黄国弟, 宋红霞   

  1. 广西壮族自治区亚热带作物研究所,广西 南宁 530001
  • 收稿日期:2019-10-29 出版日期:2020-05-25 发布日期:2020-05-29
  • 通讯作者: *,欧克纬,E-mail:707008920@qq.com
  • 作者简介:张宇(1983—),男,河北博野人,硕士,高级农艺师,主要从事热带果树的遗传育种等研究工作。 E-mail:375900554@qq.com.
  • 基金资助:
    广西自然科学基金(2016GXNSFAA380164); 广西亚热带作物研究所基本科研业务费专项(桂热研201905)

Analysis on genetic relationship of mango (Mangifera indica L.) germplasms by CDDP markers

ZHANG Yu, ZHANG Ji, RONG Tao, OU Kewei*, HUANG Guodi, SONG Hongxia   

  1. Guangxi Subtropical Crops Research Institute, Nanning 530001, China
  • Received:2019-10-29 Online:2020-05-25 Published:2020-05-29

摘要: 利用保守DNA衍生多态性(CDDP)分子标记技术,对广西亚热带作物研究所保存的31个杧果品种进行遗传多样性分析,为杧果种质资源的鉴定及分子辅助育种提供理论依据。筛选出8条CDDP引物对供试的杧果品种进行PCR扩增,分析电泳图,并进行聚类分析和主成分分析。从31个杧果品种中共扩增出107条清晰条带,其中多态性条带(NPB)100条,多态性比率(PPB)达到93.57%。平均每条引物可扩增出13条带和12条多态性带。每个位点的平均等位基因数(Na)、有效等位基因数(Ne)、Nei's基因多样性(H)、Shannon信息指数(I)分别为1.847 1、1.636 8、0.410 8、0.84,表明杧果品种间具有丰富的遗传多样性。供试杧果品种间相似性系数范围为0.620 6~0.920 8。UPGMA聚类结果表明,在遗传相似系数0.690 6处可以将供试杧果划分为7类,主成分分析结果与聚类分析结果基本一致,划分结果与杧果来源地无密切关系。筛选获得的CDDP引物对杧果种质资源有较高的多态性检测率,适用于资源鉴别及亲缘关系分析。

关键词: 杧果, CDDP, 聚类分析, 主成分分析

Abstract: To analyze the genetic diversity of 31 mango varieties and provide theoretical basis for the identification of mango germplasm resources and molecular assisted breeding, the genetic diversity of 31 mango varieties preserved in Guangxi Subtropical Crops Research Institute was analyzed by conserved DNA-derived polymorphism(CDDP) molecular marker technique. Eight CDDP primers were selected for PCR amplification of mango species tested, electrophoresis, cluster analysis and principal component analysis. There were 107 clear bands amplified from 31 mango varieties, with number of polymorphic bands (NPB) of 100 and polymorphism rate (PPB) of 93.57%. On average, 13 bands and 12 polymorphism bands were amplified from each primer. The average allele number (Na), effective allele number (Ne), Nei's gene diversity (H) and Shannon information index (I) of each locus were 1.847 1, 1.636 8, 0.410 8 and 0.84, respectively, indicating that there was rich genetic diversity among mango species. The range of similarity coefficients between mango species was 0.620 6-0.920 8. UPGMA clustering results showed that mango could be divided into 7 categories at the genetic similarity coefficient of 0.690 6. The results of principal component analysis were basically consistent with the results of cluster analysis, and the classification results were not closely related to the mango origin. The CDDP primer obtained by screening had a high polymorphism detection rate on mango germplasm resources, which was suitable for resource identification and relationship analysis.

Key words: mango, CDDP, clustering analysis, principal component analysis

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