烤后健康烟叶和霉烂烟叶真菌群落结构分析

doi:10.3969/j.issn.1004-1524.2020.06.10

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (6): 1019-1028.DOI: 10.3969/j.issn.1004-1524.2020.06.10

烤后健康烟叶和霉烂烟叶真菌群落结构分析

陈乾丽^1,2, 汪汉成², 梁永进^{3, *}, 蔡刘体², 黄宇^1,2, 周浩⁴, 李忠¹, 韩洁⁵

1.贵州大学农学院,贵州贵阳 550025;
2.贵州省烟草科学研究院,贵州贵阳 550081;
3.广西中烟工业有限责任公司,广西南宁530001;
4.长江大学农学院,湖北荆州 434025;
5.贵州中医药大学基础医学院,贵州贵阳 550025

收稿日期:2019-10-10 出版日期:2020-06-25 发布日期:2020-06-24
通讯作者: *梁永进,E-mail:417557932@qq.com
作者简介:陈乾丽(1993—),女,贵州遵义人,硕士研究生,主要从事植物病理学研究。E-mail:767761826@qq.com
基金资助:
广西中烟工业有限责任公司科技项目(201852010064028); 贵州市科学技术基金(黔科合J字〔2012〕2065号)

Fungal composition and diversity analysis of healthy and rotten tobacco leaves after curing

CHEN Qianli^1,2, WANG Hancheng², LIANG Yongjin^{3, *}, CAI Liuti², HUANG Yu^1,2, ZHOU Hao⁴, LI Zhong¹, HAN Jie⁵

1. College of Agriculture, Guizhou University, Guiyang 550025, China;
2. Guizhou Academy of Tobacco Science, Guiyang 550081, China;
3. Guangxi Zhongyan Industry Co. Ltd., Nanning 530001, China;
4. College of Life Sciences, Yangtze University, Jingzhou 434025, China;
5. College of Basic Medical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China

Received:2019-10-10 Online:2020-06-25 Published:2020-06-24

摘要/Abstract

摘要： 为了解烤后健康烟叶和霉烂烟叶真菌群落多样性,采用Illumina MiSeq测序平台对烤后健康与霉烂烟叶样品上真菌群落的ITS1-ITS2区域PCR扩增片段进行测序并分析。健康烟叶组(MJ)和霉烂烟叶组(MB)2类样品真菌主要为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、接合菌门(Zygomycota)等6个门25纲58目187科260个属的394个真菌分类单元(OTUs)。在属水平上,健康烟叶组(MJ)优势真菌为链格孢属(Alternaria),其次为曲霉属(Aspergillus)、新凸轮孢菌属(Neocamarosporium)、赤霉属(Gibberella)、支顶孢属(Acremonium);霉烂烟叶组(MB)优势真菌为曲霉属(Aspergillus),其次为青霉属(Penicillium)和根霉属(Rhizopus)。Alpha指数表明,健康烟叶组(MJ)真菌群落丰富度与多样性均高于霉烂烟叶组(MB)。71.06%的OTUs归属于8个功能类群,其中腐生菌是主要功能类群,研究结果揭示了烤后健康烟叶和霉烂烟叶真菌群落结构,为制定相应的防治方案提供参考依据。

关键词: 霉烂烟叶, 真菌ITS区, 群落结构, Illumina高通量测序

Abstract: To explore difference of the fungal community composition of healthy and rotten tobacco leaves after curing, the fungal community composition of both healthy and tobacco pole rot samples from flue-cured tobacco leaves were investigated by using PCR amplification of the ITS1-ITS2 region. The amplified fragments were sequenced using Illumina Miseq high-throughput sequencing technique. It was shown that the fungi from healthy tobacco (MJ), rotten tobacco (MB) were distributed in 6 phyla (Ascomycota, Basidiomycota, Zygomycota, etc), 25 classes, 58 orders, 187 families, 260 genera and 394 fungal taxa (OTUs). The dominant genus of MJ was Alternaria, followed by Aspergillus, Neocamarosporium, Gibberella and Acremonium. For MB, the dominant genus was Aspergillus, followed by Penicillium and Rhizopus. Alpha index showed that the fungal community richness and diversity of MJ were higher than those of MB. Additionally, functional assignments were made, the results showed that 71.06% of total OTUs belonged to eight trophic modes, of which saprotroph was the main group. The results of this study revealed the community structure of healthy and rotten tobacco leaves after curing and provided reference basis for formulating the corresponding control plan.

Key words: rotten tobacco leaf, fungal ITS area, community structure, Illumina high throughput sequencing

中图分类号:

S572

陈乾丽, 汪汉成, 梁永进, 蔡刘体, 黄宇, 周浩, 李忠, 韩洁. 烤后健康烟叶和霉烂烟叶真菌群落结构分析[J]. 浙江农业学报, 2020, 32(6): 1019-1028.

CHEN Qianli, WANG Hancheng, LIANG Yongjin, CAI Liuti, HUANG Yu, ZHOU Hao, LI Zhong, HAN Jie. Fungal composition and diversity analysis of healthy and rotten tobacco leaves after curing[J]. , 2020, 32(6): 1019-1028.

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烤后健康烟叶和霉烂烟叶真菌群落结构分析

Fungal composition and diversity analysis of healthy and rotten tobacco leaves after curing

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