三穗鸭PRKCB基因内含子14的变异对蛋壳品质的影响

doi:10.3969/j.issn.1004-1524.2020.09.06

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (9): 1574-1580.DOI: 10.3969/j.issn.1004-1524.2020.09.06

三穗鸭PRKCB基因内含子14的变异对蛋壳品质的影响

廖朝美, 游敏芳, 谭光辉, 李杰章, 张依裕^*, 覃媛钰, 刘若余

贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳 550025

收稿日期:2020-01-08 出版日期:2020-09-25 发布日期:2020-10-10
通讯作者: *张依裕,E-mail:zyy8yyc@163.com
作者简介:廖朝美(1996—),女,贵州黄平人,硕士研究生,研究方向为动物遗传育种与繁殖。E-mail:3285438093@qq.com
基金资助:
国家自然科学基金(31760663);贵州省科技人才补助项目(黔科合平台人才〔2017〕5788);贵州省千层次创新型人才项目(701030174401)

Effect of variation of PRKCB gene intron 14 in Sansui ducks on eggshell quality

LIAO Chaomei, YOU Minfang, TAN Guanghui, LI Jiezhang, ZHANG Yiyu^*, QIN Yuanyu, LIU Ruoyu

Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China

Received:2020-01-08 Online:2020-09-25 Published:2020-10-10

摘要/Abstract

摘要： 为探究PRKCB基因对三穗鸭蛋壳品质的遗传效应,采用PCR产物直接测序法、DNAStar软件MegaAlign程序结合Chromas软件对120只三穗鸭(Anas platyrhyncha domestica)PRKCB基因的遗传位点变异进行筛查鉴定,用SPSS 18.0软件分析SNP位点与蛋壳品质的相关性。结果显示:在三穗鸭PRKCB基因第14内含子检测到g.1158465 C>T、g.1158519 G>A和g.1158524 G>A这3个SNPs突变位点,每个突变位点均产生3种基因型,且均为中度多态位点。g.1158519 G>A和g.1158524 G>A突变位点的基因型分布均符合Hardy-Weinberg平衡,g.1158465 C>T位点的基因型分布则显著(P<0.05)偏离Hardy-Weinberg平衡;g.1158519 G>A和g.1158465 C>T位点之间存在强连锁不平衡。3个SNPs共组成5种单倍型和8种双倍型,优势单倍型H5(TGG)的频率为0.492,优势双倍型H2H5频率为0.200。关联分析显示,g.1158519 G>A位点的AG基因型个体蛋壳厚度显著(P<0.05)高于GG基因型个体,双倍型H1H3个体的蛋壳厚度显著(P<0.05)高于H3H5个体的蛋壳厚度。研究结果提示,PRKCB基因的遗传变异可以影响蛋壳品质,其中g.1158519 G>A位点可作为改善三穗鸭蛋壳品质选择的遗传标记。

关键词: PRKCB基因, 三穗鸭, SNP鉴定, 蛋壳品质, 关联分析

Abstract: To explore the genetic effect of PRKCB gene on eggshell quality of Sansui duck, direct sequencing of PCR products, MegaAlign program of DNAStar software and Chromas software were used to screen and identify the genetic locus variation of (Anas platyrhyncha domestica) PRKCB gene. The correlation between SNP loci and eggshell traits were analysed by SPSS 18.0 software. The results indicated that three moderately polymorphic novel SNPs of g.1158465 C>T, g.1158519 G>A and g.1158524 G>A were detected in intron 14 of PRKCB gene in Sansui duck. Three genotypes were produced in each SNP. The genotype distribution of g.1158519 G>A and g.1158524 G>A were in accordance with Hardy-Weinberg equilibrium, while that of g.1158465 C>T was significantly (P<0.05) deviated from Hardy-Weinberg equilibrium. There was a strong linkage disequilibrium between g.1158519 G>A and g.1158465 C>T loci. The combination of three SNPs composed of 5 haplotypes and 8 diplotypes. The frequency of haplotype H5 (TGG) and diplotype H2H5 was 0.492 and 0.200, respectively. Association analysis showed that the eggshell thickness of individuals with AG genotype at g.1158519 G>A locus was significantly (P<0.05) higher than that of individuals with GG genotype, and the eggshell thickness of diplotype H1H3 individuals was significantly (P<0.05) higher than that of H3H5 individuals. The results suggested that the genetic variation of the intron 14 in PRKCB gene can affect duck eggshell quality, and g.1158519 G>A locus may be used as a genetic marker to improve duck eggshell quality.

Key words: PRKCB gene, Sansui ducks, SNP identification, eggshell quality, association analysis

中图分类号:

S834

廖朝美, 游敏芳, 谭光辉, 李杰章, 张依裕, 覃媛钰, 刘若余. 三穗鸭PRKCB基因内含子14的变异对蛋壳品质的影响[J]. 浙江农业学报, 2020, 32(9): 1574-1580.

LIAO Chaomei, YOU Minfang, TAN Guanghui, LI Jiezhang, ZHANG Yiyu, QIN Yuanyu, LIU Ruoyu. Effect of variation of PRKCB gene intron 14 in Sansui ducks on eggshell quality[J]. , 2020, 32(9): 1574-1580.

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三穗鸭PRKCB基因内含子14的变异对蛋壳品质的影响

Effect of variation of PRKCB gene intron 14 in Sansui ducks on eggshell quality

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