魏可葡萄气生根发育的转录组分析

doi:10.3969/j.issn.1004-1524.2020.09.13

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (9): 1645-1655.DOI: 10.3969/j.issn.1004-1524.2020.09.13

魏可葡萄气生根发育的转录组分析

葛金涛, 王江英, 赵文静, 邵小斌, 朱朋波, 汤雪燕, 孙明伟, 刘兴满^*

连云港市农业科学院,江苏连云港 222000

收稿日期:2020-03-13 出版日期:2020-09-25 发布日期:2020-10-10
通讯作者: *刘兴满,E-mail:13611551930@163.com
作者简介:葛金涛(1987—),男,山东安丘人,硕士,助理研究员,主要从事果树遗传育种研究。E-mail:672643419@qq.com
基金资助:
江苏省农业科学院探索性颠覆性创新计划(ZX(17)2003)

Transcriptome analysis on development of aerial root in grape of Weike

GE Jintao, WANG Jiangying, ZHAO Wenjing, SHAO Xiaobin, ZHU Pengbo, TANG Xueyan, SUN Mingwei, LIU Xingman^*

Lianyungang Academy of Agricultural Sciences, Lianyungang 222000, China

Received:2020-03-13 Online:2020-09-25 Published:2020-10-10

摘要/Abstract

摘要： 葡萄气生根是在高温高湿环境下老蔓上发育形成的一种特殊根系类型,发育程度较低,具有分化潜力。通过技术手段诱导魏可葡萄当年生结果枝茎节处萌发气生根,以同一结果母枝未处理茎节为对照进行根系转录基因表达情况分析,结果表明,诱导气生根发育过程中有3 989个基因表达量上升,2 830个基因表达量下调。采用GO功能分类,可将注释的基因划分为40个功能类别,包括催化活性、结合和代谢过程等。部分根系关键基因表达结果表明,生长素、赤霉素、脱落酸在内的所有植物激素都参与了调控过程,结合生长素下游相应基因分析,内切葡聚糖酶基因、PRE基因和高亲和力硝酸盐转运体基因在气生根发育过程中显著上调表达,表明降解茎节处表皮细胞壁、利用硝酸盐在茎节处富集刺激根芽萌发是气生根生长发育调控机理之一。

关键词: 葡萄, 气生根, 根系, 转录组测序, 生长素

Abstract: Aerial root is a special type of root in grape which grows under high temperature and high humidity. The aerial root has low developmental degree and high potential for differentiation. In this study, techniques were used to induce aerial germination and rooting at the stem nodes of the fruiting branch of Weike grape, and the expression of root transcription genes was analyzed with the untreated stem nodes of the same mother branch as the control. The results showed that the expression of 3 989 genes was increased and the expression of 2 830 genes was down-regulated during the induction of aerial roots. GO functional classification was used to classify annotated genes into 40 functional categories, including catalytic activity, binding, and metabolic processes. Parts of the root key gene expression results showed that all plant hormones including auxin, gibberellin and abscisic acid were involved in the regulation process. Combined with corresponding gene analysis of auxin downstream, endoglucanase, PRE gene and high-affinity nitrate transporter gene significantly up-regulated in the aerial root development, indicating that the degradation of epidermal cell wall at stem nodes and the enrichment of nitrate at stem nodes to stimulate root germination were important regulatory mechanisms for the growth and development of aerial roots.

Key words: grape, aerial root, root system, transcriptome sequencing, auxin

中图分类号:

S663.1

葛金涛, 王江英, 赵文静, 邵小斌, 朱朋波, 汤雪燕, 孙明伟, 刘兴满. 魏可葡萄气生根发育的转录组分析[J]. 浙江农业学报, 2020, 32(9): 1645-1655.

GE Jintao, WANG Jiangying, ZHAO Wenjing, SHAO Xiaobin, ZHU Pengbo, TANG Xueyan, SUN Mingwei, LIU Xingman. Transcriptome analysis on development of aerial root in grape of Weike[J]. , 2020, 32(9): 1645-1655.

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魏可葡萄气生根发育的转录组分析

Transcriptome analysis on development of aerial root in grape of Weike

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