衣藻和固氮鱼腥藻对盐胁迫下小麦幼苗生长的影响

doi:10.3969/j.issn.1004-1524.2020.10.02

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (10): 1748-1756.DOI: 10.3969/j.issn.1004-1524.2020.10.02

衣藻和固氮鱼腥藻对盐胁迫下小麦幼苗生长的影响

边建文^1,2, 崔岩², 杨宋琪², 罗光宏^2,*, 孟宪刚^1,*

1.兰州交通大学化学与生物工程学院,甘肃兰州 730070;
2.河西学院甘肃省微藻工程技术研究中心,甘肃张掖 734000

收稿日期:2020-04-10 出版日期:2020-10-25 发布日期:2020-11-16
通讯作者: *孟宪刚,E-mail: mengxg@mail.lzjtu.cn;罗光宏,E-mail: kyluo@hxu.edu.cn
作者简介:边建文(1992—),男,甘肃武威人,硕士研究生,研究方向为微生物与发酵工程。E-mail: 1004192852@qq.com
基金资助:
国家自然科学基金(11665011); 甘肃省高校协同创新科技团队支持计划(2017C-17); 甘肃省微藻技术创新中心建设项目(18JR3JG001); 甘肃省高校科研项目(2020B-213); 河西学院博士启动基金; 河西学院校长科研创新基金重点项目(XZZD2018002)

Effects of Chlamydomonas debaryana Gor. and Anabaena azotica Ley. on wheat seedling growth under salt stress

BIAN Jianwen^1,2, CUI Yan², YANG Songqi², LUO Guanghong^2,*, MENG Xiangang^1,*

1. College of Chemistry and Bioengineering, Lanzhou Jiaotong University, Lanzhou 730070, China;
2. Gansu Microalgae Engineering Technology Research Center, Hexi University, Zhangye 734000, China

Received:2020-04-10 Online:2020-10-25 Published:2020-11-16

摘要/Abstract

摘要： 以小麦为试验材料,研究了向土壤中添加衣藻(Chlamydomonas debaryana Gor.)、固氮鱼腥藻(Anabaena azotica Ley.)或其混合藻液对盐胁迫下小麦幼苗生长的影响。结果表明:50 mmol·L^-1 NaCl可以促进小麦茎长和根长的生长,但在100、200 mmol·L^-1 NaCl处理下,小麦生长受到抑制,茎长、根长、鲜质量、叶绿素含量和光系统Ⅱ最大光化学效率(F_v/F_m)值下降。添加微藻藻液可以缓解盐胁迫对小麦生长的抑制作用,提高盐胁迫下小麦的茎长、根长、鲜质量、叶绿素含量和光合作用效率,增强其抗氧化酶——超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性来清除活性氧,从而提高小麦的耐盐性。

关键词: 微藻, 盐胁迫, 小麦, 叶绿素荧光参数, 抗氧化酶活性

Abstract: In the present study, wheat seedlings were used as the experimental material, to explore the effects of microalgae (Chlamydomonas debaryana Gor., Anabaena azotica Ley., and their mixture) on the growth of wheat seedlings under salt stress. It was shown that 50 mmol·L^-1 NaCl could promote the growth of wheat stem and root. Under 100, 200 mmol·L^-1 NaCl, the growth of wheat was inhibited, as the stem length, root length, fresh weight, chlorophyll content and the value of maximal photochemistry efficiency of PSⅡ(F_v/F_m) decreased. The addition of microalgae could alleviate the inhibition of salt stress on wheat growth, by increasing the stem length, root length, fresh weight, chlorophyll content and photosynthesis efficiency of wheat, and enhance the activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) to remove active oxygen, so as to improve the salt tolerance of wheat.

Key words: microalgae, salt stress, wheat, chlorophyll fluorescence parameter, antioxidant enzymes activity

中图分类号:

S512

边建文, 崔岩, 杨宋琪, 罗光宏, 孟宪刚. 衣藻和固氮鱼腥藻对盐胁迫下小麦幼苗生长的影响[J]. 浙江农业学报, 2020, 32(10): 1748-1756.

BIAN Jianwen, CUI Yan, YANG Songqi, LUO Guanghong, MENG Xiangang. Effects of Chlamydomonas debaryana Gor. and Anabaena azotica Ley. on wheat seedling growth under salt stress[J]. , 2020, 32(10): 1748-1756.

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衣藻和固氮鱼腥藻对盐胁迫下小麦幼苗生长的影响

Effects of Chlamydomonas debaryana Gor. and Anabaena azotica Ley. on wheat seedling growth under salt stress

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