浙江农业学报 ›› 2021, Vol. 33 ›› Issue (3): 383-389.DOI: 10.3969/j.issn.1004-1524.2021.03.01
收稿日期:
2020-11-13
出版日期:
2021-04-02
发布日期:
2021-03-25
通讯作者:
邱红波
作者简介:
, 邱红波,E-mail: qhb001122@163.com基金资助:
YANG Mei, HU Xiaolan, SHEN Tao, TAN Kang, LIU Dailing, QIU Hongbo*()
Received:
2020-11-13
Online:
2021-04-02
Published:
2021-03-25
Contact:
QIU Hongbo
摘要:
以贵州省常用玉米自交系T32为供体,J51为受体,通过连续多代回交并结合分子标记辅助选择,获得了23个玉米第8染色体单片段代换系。采用自然接种法对该群体进行灰斑病抗性鉴定,并选用在两亲本间均具明显多态性差异的65对SSR标记进行代换系的跟踪检测,通过第8染色体上的29个SSR标记对代换系供体片段进行遗传结构分析。结果表明,23个染色体单片段代换系中供体代换的位点不同,代换片段长度为10.40~129.70 cM,平均长度为36.99 cM,导入片段总长850.67 cM,对第8染色体的覆盖率为80.80%。自然接种灰斑病菌后产生了抗、中抗、感、高感4种表型,其中H3系和H17系发病程度较低,2年的病级均值分别为2.85、2.90,表现为抗病。研究筛选出的2个抗性株系可作为后续研究材料,为玉米灰斑病抗性基因挖掘与抗病育种创建基础。
中图分类号:
杨梅, 胡小兰, 申涛, 谭康, 刘代铃, 邱红波. 玉米第8染色体单片段代换系的构建与灰斑病抗性材料筛选[J]. 浙江农业学报, 2021, 33(3): 383-389.
YANG Mei, HU Xiaolan, SHEN Tao, TAN Kang, LIU Dailing, QIU Hongbo. Construction of single fragment substitution lines of maize 8th chromosome and sreening of resistant maize germplasm to gray leaf spot[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 383-389.
图1 构建染色体片段代换系群体试验方案 T32,供体亲本;J51,受体亲本;F1,两亲本杂交产生的杂种第一代;BC1F1,F1与受体亲本回交产生的回交1代;BC2F1,BC1F1与受体亲本回交产生的回交2代;BC5F1,BC4F1与受体亲本回交产生的回交5代;BC5F2,回交5次的自交2代;MAS,分子标记辅助选择。
Fig.1 Experimental program of constructing chromosome fragment substitution lines T32 was donor parent; J51 was recipient parent; F1 was the first generation of the hybrid produced by the cross between the two parents; BC1F1 was the first generation of backcross that F1 was backcrossed with the recipient parent; BC2F1 was the second generation of the backcross of BC1F1 and the recipient parent; BC5F1 was the backcross of BC4F1 and the recipient parent, resulting in the backcrossing of five generations; BC5F2 was the second generation of selfing that was backcrossed five times; MAS was molecular marker-assisted selection.
图2 代换片段长度计算示意图 X为标记;LMIN为代换片段的最小长度;LMAX为代换片段的最大长度;L为代换片段的估计长度。
Fig.2 Schematic diagram of calculating the length of the substitution segment X, Marker; LMIN, Minimum length of substitution segment; LMAX, Maximum length of substitution segment; L, Estimated length of substitution segment.
染色体片 段代换 CSILs | 病级Rating | 抗性评价 Resistance | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
2018年 2018 year | 2019年2019 year | 平均 Average | |||||||||
勐嘎Mengga | 江东Jiangdong | 勐嘎Mengga | 江东Jiangdong | ||||||||
标准差s | 变异范围 Range of variation | 标准差s | 变异范围 Range of variation | 标准差s | 变异范围 Range of variation | 标准差s | 变异范围 Range of variation | ||||
J51 | 0 | 9~9 | 0 | 9~9 | 0 | 9~9 | 0 | 9~9 | 9.00 | HS | |
T32 | 0 | 1~1 | 0 | 1~1 | 0 | 1~1 | 0 | 1~1 | 1.00 | HR | |
H1 | 1.00 | 5~7 | 1.34 | 5~7 | 0.98 | 5~7 | 0.98 | 5~7 | 5.90 | MR | |
H2 | 1.00 | 7~9 | 1.33 | 5~9 | 0.92 | 7~9 | 0 | 9~9 | 8.30 | HS | |
H3 | 1.08 | 1~5 | 0.80 | 1~3 | 1.50 | 1~3 | 1.40 | 1~7 | 2.85 | R | |
H4 | 1.00 | 7~9 | 0.98 | 7~9 | 0.80 | 7~9 | 0.98 | 7~9 | 7.85 | HS | |
H5 | 1.20 | 5~9 | 0.60 | 7~9 | 0.80 | 7~9 | 0.92 | 7~9 | 8.60 | HS | |
H6 | 0.60 | 7~9 | 0.60 | 7~9 | 0.80 | 7~9 | 0.92 | 7~9 | 8.15 | HS | |
H7 | 1.08 | 3~7 | 0.98 | 5~7 | 1.00 | 5~9 | 0.98 | 5~7 | 5.80 | MR | |
H8 | 0.80 | 7~9 | 0 | 9~9 | 0 | 9~9 | 0.60 | 7~9 | 8.85 | HS | |
H9 | 1.00 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 8.05 | HS | |
H10 | 0.92 | 7~9 | 0 | 9~9 | 0.60 | 7~9 | 0.80 | 7~9 | 8.70 | HS | |
H11 | 0.98 | 7~9 | 0.80 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 8.30 | HS | |
H12 | 0 | 9~9 | 0.92 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 8.25 | HS | |
H13 | 0.92 | 7~9 | 0.60 | 7~9 | 0.80 | 7~9 | 0.80 | 7~9 | 8.60 | HS | |
H14 | 0.80 | 7~9 | 1.28 | 5~9 | 0.92 | 7~9 | 0.60 | 7~9 | 7.95 | HS | |
H15 | 1.00 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 0.92 | 7~9 | 8.20 | HS | |
H16 | 1.28 | 5~9 | 1.00 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 7.80 | HS | |
H17 | 0.60 | 1~3 | 0.60 | 1~3 | 0 | 3~3 | 0 | 3~3 | 2.90 | R | |
H18 | 0.60 | 7~9 | 0 | 7~7 | 1.20 | 5~9 | 0.98 | 5~7 | 6.75 | S | |
H19 | 1.33 | 5~9 | 0.80 | 7~9 | 1.00 | 7~9 | 0.92 | 7~9 | 8.20 | HS | |
H20 | 0.92 | 7~9 | 0.00 | 9~9 | 0.92 | 7~9 | 0.60 | 7~9 | 8.05 | HS | |
H21 | 0.98 | 7~9 | 0.80 | 7~9 | 0.92 | 7~9 | 1.00 | 7~9 | 8.20 | HS | |
H22 | 0.60 | 7~9 | 0.92 | 7~9 | 0.80 | 7~9 | 0.98 | 7~9 | 8.50 | HS | |
H23 | 0.60 | 7~9 | 0.80 | 7~9 | 0.60 | 7~9 | 0.92 | 7~9 | 8.65 | HS |
表1 染色体片段代换系玉米灰斑病的抗性鉴定
Table 1 Identification of gray spot disease resistance of chromosome segment substitution lines
染色体片 段代换 CSILs | 病级Rating | 抗性评价 Resistance | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
2018年 2018 year | 2019年2019 year | 平均 Average | |||||||||
勐嘎Mengga | 江东Jiangdong | 勐嘎Mengga | 江东Jiangdong | ||||||||
标准差s | 变异范围 Range of variation | 标准差s | 变异范围 Range of variation | 标准差s | 变异范围 Range of variation | 标准差s | 变异范围 Range of variation | ||||
J51 | 0 | 9~9 | 0 | 9~9 | 0 | 9~9 | 0 | 9~9 | 9.00 | HS | |
T32 | 0 | 1~1 | 0 | 1~1 | 0 | 1~1 | 0 | 1~1 | 1.00 | HR | |
H1 | 1.00 | 5~7 | 1.34 | 5~7 | 0.98 | 5~7 | 0.98 | 5~7 | 5.90 | MR | |
H2 | 1.00 | 7~9 | 1.33 | 5~9 | 0.92 | 7~9 | 0 | 9~9 | 8.30 | HS | |
H3 | 1.08 | 1~5 | 0.80 | 1~3 | 1.50 | 1~3 | 1.40 | 1~7 | 2.85 | R | |
H4 | 1.00 | 7~9 | 0.98 | 7~9 | 0.80 | 7~9 | 0.98 | 7~9 | 7.85 | HS | |
H5 | 1.20 | 5~9 | 0.60 | 7~9 | 0.80 | 7~9 | 0.92 | 7~9 | 8.60 | HS | |
H6 | 0.60 | 7~9 | 0.60 | 7~9 | 0.80 | 7~9 | 0.92 | 7~9 | 8.15 | HS | |
H7 | 1.08 | 3~7 | 0.98 | 5~7 | 1.00 | 5~9 | 0.98 | 5~7 | 5.80 | MR | |
H8 | 0.80 | 7~9 | 0 | 9~9 | 0 | 9~9 | 0.60 | 7~9 | 8.85 | HS | |
H9 | 1.00 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 8.05 | HS | |
H10 | 0.92 | 7~9 | 0 | 9~9 | 0.60 | 7~9 | 0.80 | 7~9 | 8.70 | HS | |
H11 | 0.98 | 7~9 | 0.80 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 8.30 | HS | |
H12 | 0 | 9~9 | 0.92 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 8.25 | HS | |
H13 | 0.92 | 7~9 | 0.60 | 7~9 | 0.80 | 7~9 | 0.80 | 7~9 | 8.60 | HS | |
H14 | 0.80 | 7~9 | 1.28 | 5~9 | 0.92 | 7~9 | 0.60 | 7~9 | 7.95 | HS | |
H15 | 1.00 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 0.92 | 7~9 | 8.20 | HS | |
H16 | 1.28 | 5~9 | 1.00 | 7~9 | 0.98 | 7~9 | 0.98 | 7~9 | 7.80 | HS | |
H17 | 0.60 | 1~3 | 0.60 | 1~3 | 0 | 3~3 | 0 | 3~3 | 2.90 | R | |
H18 | 0.60 | 7~9 | 0 | 7~7 | 1.20 | 5~9 | 0.98 | 5~7 | 6.75 | S | |
H19 | 1.33 | 5~9 | 0.80 | 7~9 | 1.00 | 7~9 | 0.92 | 7~9 | 8.20 | HS | |
H20 | 0.92 | 7~9 | 0.00 | 9~9 | 0.92 | 7~9 | 0.60 | 7~9 | 8.05 | HS | |
H21 | 0.98 | 7~9 | 0.80 | 7~9 | 0.92 | 7~9 | 1.00 | 7~9 | 8.20 | HS | |
H22 | 0.60 | 7~9 | 0.92 | 7~9 | 0.80 | 7~9 | 0.98 | 7~9 | 8.50 | HS | |
H23 | 0.60 | 7~9 | 0.80 | 7~9 | 0.60 | 7~9 | 0.92 | 7~9 | 8.65 | HS |
株系编号 Lines number | 供体片段位点 Donor fragment site | 最短长度 Minimum length/cM | 最长长度 Maximum length/cM | 估计长度 Estimated length/cM |
---|---|---|---|---|
H1 | umc2356 | 0 | 63.20 | 31.60 |
H2 | umc2356-bnlg1607-umc1607-umc266d-bnlg1828 | 93.90 | 165.50 | 129.70 |
H3 | umc1034-csu329 | 1.87 | 62.40 | 32.13 |
H4 | umc1487 | 0 | 67.99 | 33.00 |
H5 | umc2354 | 0 | 39.90 | 19.95 |
H6 | umc1130-umc2367 | 14.40 | 51.60 | 33.00 |
H7 | umc2218 | 0 | 64.70 | 32.35 |
H8 | umc226d | 0 | 40.30 | 20.15 |
H9 | umc1414 | 0 | 80.50 | 40.25 |
H10 | umc2352a-bnlg1073-umc1304 | 0 | 21.30 | 10.65 |
H11 | bnlg1828-umc1807 | 14.80 | 36.81 | 25.81 |
H12 | bnlg1828 | 0 | 31.01 | 15.51 |
H13 | bnlg2046 | 0 | 25.90 | 12.95 |
H14 | umc2354-bnlg1863-bnlg1460-umc1302 | 44.20 | 62.58 | 53.39 |
H15 | phi080 | 0 | 24.30 | 12.15 |
H16 | bnlg1607-umc1607 | 21.60 | 77.73 | 49.67 |
H17 | umc1034-csu329-bnlg1828-umc1807-umc2354 | 38.68 | 87.60 | 63.14 |
H18 | umc1384 | 0 | 56.67 | 28.34 |
H19 | bnlg1828-umc1807-umc1377-bnlg1460 | 29.68 | 88.00 | 58.84 |
H20 | umc1139 | 0 | 26.80 | 13.40 |
H21 | umc2367-bnlg2181-umc1889-umc1316 | 37.20 | 82.80 | 60.00 |
H22 | umc1807-umc2354-bnlg1863-bnlg1828-umc1807-umc1377 | 54.60 | 84.38 | 69.49 |
H23 | umc1377 | 0 | 10.40 | 5.20 |
表2 代换片段系的遗传分析
Table 2 Genetic analysis of substitution lines
株系编号 Lines number | 供体片段位点 Donor fragment site | 最短长度 Minimum length/cM | 最长长度 Maximum length/cM | 估计长度 Estimated length/cM |
---|---|---|---|---|
H1 | umc2356 | 0 | 63.20 | 31.60 |
H2 | umc2356-bnlg1607-umc1607-umc266d-bnlg1828 | 93.90 | 165.50 | 129.70 |
H3 | umc1034-csu329 | 1.87 | 62.40 | 32.13 |
H4 | umc1487 | 0 | 67.99 | 33.00 |
H5 | umc2354 | 0 | 39.90 | 19.95 |
H6 | umc1130-umc2367 | 14.40 | 51.60 | 33.00 |
H7 | umc2218 | 0 | 64.70 | 32.35 |
H8 | umc226d | 0 | 40.30 | 20.15 |
H9 | umc1414 | 0 | 80.50 | 40.25 |
H10 | umc2352a-bnlg1073-umc1304 | 0 | 21.30 | 10.65 |
H11 | bnlg1828-umc1807 | 14.80 | 36.81 | 25.81 |
H12 | bnlg1828 | 0 | 31.01 | 15.51 |
H13 | bnlg2046 | 0 | 25.90 | 12.95 |
H14 | umc2354-bnlg1863-bnlg1460-umc1302 | 44.20 | 62.58 | 53.39 |
H15 | phi080 | 0 | 24.30 | 12.15 |
H16 | bnlg1607-umc1607 | 21.60 | 77.73 | 49.67 |
H17 | umc1034-csu329-bnlg1828-umc1807-umc2354 | 38.68 | 87.60 | 63.14 |
H18 | umc1384 | 0 | 56.67 | 28.34 |
H19 | bnlg1828-umc1807-umc1377-bnlg1460 | 29.68 | 88.00 | 58.84 |
H20 | umc1139 | 0 | 26.80 | 13.40 |
H21 | umc2367-bnlg2181-umc1889-umc1316 | 37.20 | 82.80 | 60.00 |
H22 | umc1807-umc2354-bnlg1863-bnlg1828-umc1807-umc1377 | 54.60 | 84.38 | 69.49 |
H23 | umc1377 | 0 | 10.40 | 5.20 |
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