2份春小麦种质资源成株期抗条锈病基因遗传分析

doi:10.3969/j.issn.1004-1524.2021.04.04

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (4): 595-601.DOI: 10.3969/j.issn.1004-1524.2021.04.04

2份春小麦种质资源成株期抗条锈病基因遗传分析

赵珂¹(), 李秋荣¹, 侯璐¹^,^*(), 白耀博², 蒋礼玲¹, 魏有海¹, 郭青云¹

1.青海大学青海省农林科学院青海省农业有害生物综合治理重点实验室,青海西宁 810016
2.徐州生物工程职业技术学院徐州现代农业生物技术重点实验室,江苏徐州221006

收稿日期:2020-12-23 出版日期:2021-04-25 发布日期:2021-04-25
通讯作者: 侯璐
作者简介:*侯璐,E-mail:mantou428@163.com
赵珂(1992—),女,安徽宿州人,硕士研究生,从事植物病理研究。E-mail:18355095979@163.com
基金资助:
国家自然科学基金(31660513);第二次青藏高原综合科学考察研究(2019QZKK0303);青海省农业有害生物综合治理重点实验室(2020-ZJ-Y11);青海省农业有害生物综合治理重点实验室(2021-ZJ-Y08);农业农村部农业资源环境保护项目(125A0605)

Genetic analysis of stripe rust resistance genes in 2 spring wheat germplasm resources at adult stage

ZHAO Ke¹(), LI Qiurong¹, HOU Lu¹^,^*(), BAI Yaobo², JIANG Liling¹, WEI Youhai¹, GUO Qingyun¹

1. Key Laboratory of Agricultural Integrated Pest Management, Qinghai University, Qinghai Academy of Agricultural and Forestry Sciences, Xining 810016, China
2. Xuzhou Key Laboratory of Modern Agrobiotechnology Engineering, Xuzhou Vocational College of Bioengineering, Xuzhou 221006, China

Received:2020-12-23 Online:2021-04-25 Published:2021-04-25
Contact: HOU Lu

摘要/Abstract

摘要：

研究分析2份春小麦种质资源成株期的抗条锈病基因遗传规律,为春小麦抗条锈病基因利用提供理论依据。采用春小麦Taichung29与MY004730杂交、ZM018243与MY004730杂交的方式分别创建F_2:3代分离群体进行2 a的田间测试,对抗条锈病基因遗传进行分析。结果表明,2个F_2:3群体的病害严重度和反应型在2个试验点均未呈现连续性分布,且均不符合正态分布。初步推测,MY004730与ZM018243对春小麦条锈病的成株期抗性具有质量性状特征。Taichung29与MY004730杂交构建的F_2:3群体单株/家系抗感分离符合由1对基因作用的3R:1S的分离比,表明MY004730的成株期抗条锈性由1对显性基因控制; ZM018243与MY004730杂交构建的F_2:3群体的单株/家系抗感分离比均符合9R:7S,即符合由2对显性基因共同作用的分离比, ZM018243中可能也含有1对显性成株期抗条锈性基因。下一步可以进行抗病基因的分子标记定位,找到与抗病基因紧密连锁的标记,为分子标记辅助选择育种服务,同时还能在抗-抗杂交群体中直接筛选抗病基因聚合材料,为育种提供优良抗病材料。

关键词: 春小麦, 抗条锈病基因, 种质资源, 遗传分析

Abstract:

Analysis of the genetic rules of the stripe rust resistance genes of spring wheat at adult stage can provide theoretical basis for the utilization of stripe rust resistance genes. In this study, stripe rust genes genetic analysis was carried out in the field for 2 years by using F_2:3 generation segregating populations made by susceptible variety Taichung29 crossed with MY004730, and ZM018243 crossed with MY004730. Results showed that, disease severity and infection type of the 2 F_2:3 populations showed discrete distribution and didn’t conform to normal distribution. It is speculated that resources the stripe rust resistance of MY004730 and ZM018243 at adult stage has qualitative character. Resistance and susceptible segregation ratio of F_2:3 population constructed by crossing of Taichung29 and MY004730 was 3R:1S, indicating that resistance of MY004730 to stripe rust at adult plant stage was controlled by 1 dominant gene; while F_2:3 population by ZM018243 crossing with MY004730, the single plants/lines resistance and susceptible segregation ratio was 9R:7S, that is conformed to 2 pairs of dominant genes work together. Therefore, it is that ZM018243 may contain 1 pair of dominant stripe rust resistance gene adult plant stage. According to the genetic analysis results, fuether research can be focused on molecular mapping of resistance genes to find markers closely linked to resistance genes, and serve for molecular marker-assisted selection breeding. At the same time, resistant gene aggregation materials can be screened from the resistance-resistance crossing population, hence provide excellent resistance materials.

Key words: spring wheat, stripe rust resistance gene, germplasm resources, genetic analysis

中图分类号:

赵珂, 李秋荣, 侯璐, 白耀博, 蒋礼玲, 魏有海, 郭青云. 2份春小麦种质资源成株期抗条锈病基因遗传分析[J]. 浙江农业学报, 2021, 33(4): 595-601.

ZHAO Ke, LI Qiurong, HOU Lu, BAI Yaobo, JIANG Liling, WEI Youhai, GUO Qingyun. Genetic analysis of stripe rust resistance genes in 2 spring wheat germplasm resources at adult stage[J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 595-601.

图/表 3

图1 Taichung 29/MY004730(A、B)与ZM018243/MY004730(C、D) F2群体反应型(A、C)和病害严重度(B、D)的正态概率图

Fig.1 Normal probability of Taichung 29/MY004730 (A) and ZM018243/MY004730 (B) F2 population response type and disease severity

图2 Taichung 29/MY004730(A、B)和ZM018243/MY004730(C、D) F2∶3群体在西宁试验地所测反应型(A、C)和病害严重度(B、D)的频率分布图

Fig.2 Frequency distribution of infection type and disease severity of F2∶3, populations from Taichung 29/MY004730 and ZM018243/MY004730tested in Xining P1,Taichung29;P2,MY004730;P3,ZM018243。

表1 Taichung 29/MY004730和ZM018243/MY004730 F2∶3群体抗病性遗传分析结果

Table 1 Genetic analysis of disease resistance in Taichung 29/MY004730 and ZM018243/MY004730 F2:3 populations

杂交组合 Cross combination	年份 Year	后代 Generation	抗病植株数 No. of resistant plants	感病植株数 No. of susceptible plants	期望比 Expected ratio(R:S)	χ²	P
Taichung29/MY004730	2017	F₂	130	45	3∶1	0.02	0.83
	2019	F₃	131	44	3∶1	<0.01	0.97
ZM018243/MY004730	2017	F₂	89	71	9∶7	<0.01	0.87
	2019	F₃	79	68	9∶7	0.28	0.54

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2份春小麦种质资源成株期抗条锈病基因遗传分析

Genetic analysis of stripe rust resistance genes in 2 spring wheat germplasm resources at adult stage

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