浙江农业学报 ›› 2021, Vol. 33 ›› Issue (7): 1234-1243.DOI: 10.3969/j.issn.1004-1524.2021.07.09

• 园艺科学 • 上一篇    下一篇

贵州茶树种质资源遗传多样性、群体结构和遗传分化研究

安红卫(), 宋勤飞, 牛素贞*()   

  1. 贵州大学 茶学院, 贵州 贵阳 550025
  • 收稿日期:2021-03-05 出版日期:2021-07-25 发布日期:2021-08-06
  • 通讯作者: 牛素贞
  • 作者简介:*牛素贞,E-mail: niusuzhen@163.com
    安红卫(1995—),女,贵州道真人,硕士研究生,主要从事茶树种质资源与遗传改良。E-mail: 2541669421@qq.com
  • 基金资助:
    贵州省自然科学基金重点项目(黔科合基础〔2019〕1404 号);国家自然科学基金(32060700);贵州省科技厅农业攻关项目(黔科合支撑〔2017〕2558);贵州省科技厅农业攻关项目(黔科合支撑〔2017〕2557);贵州省教育厅重点领域项目(黔教合KY〔2021〕042);黔西南州科技计划(2019-1-69)

Study on genetic diversity,population structure and genetic differentiation of tea germplasm in Guizhou

AN Hongwei(), SONG Qinfei, NIU Suzhen*()   

  1. College of Tea, Guizhou University, Guiyang 550025, China
  • Received:2021-03-05 Online:2021-07-25 Published:2021-08-06
  • Contact: NIU Suzhen

摘要:

以144份贵州茶树种质资源为材料,采用68个EST-SSR标记探究其遗传多样性、群体结构及遗传分化。结果表明,144份茶树种质资源按海拔范围及种都可以分为3类,Gro1代表海拔1 500 m以上的大厂茶群体,Gro2代表海拔范围1 000~1 300 m的茶群体,Gro3代表海拔不明显、种不明显的群体。144份茶树种质资源按种、地区、海拔进行遗传多样性分析发现,群体分化系数(Gst)分别为0.079 7、0.067 7、0.047 0,基因流(Nm)分别为5.774 1、6.885 2、10.134 8,表明遗传变异存在于群体内部个体间,不同群体间存在较强的基因交流。海拔差距越大,遗传距离越远,说明了海拔对于贵州野生茶树种质资源的分类有重要的影响,为更进一步探明贵州茶树种质资源的演化和分布规律奠定基础。

关键词: 茶树种质资源, 群体结构, 遗传多样性

Abstract:

In this study,68 EST-SSR primers were used to analyze the genetic diversity, population structure and genetic differentiation of 144 tea germplasm resources, which were divided into three groups by different altitude ranges and species: Gro1 represented the tea population of Camellia tachangensis(F.C.Zhang) and over 1 500 m altitude, Gro2 represented the tea population of Camellia sinensis(L.) O. Ktze and altitude from 1 000 to 1 300 m, and Gro3 represented a population whose altitude and species are not obvious. The tea germplasmresources were analyzed according to species, zones and altitude ranges, Gst was 0.079 7, 0.067 7, 0.047 0, Nm was 5.774 1, 6.885 2, 10.134 8, respectively, which showed that genetic variation existed among different individuals within populations, and there were strong gene communication among different populations.The greater the altitude gap was,the farther the genetic distance was.This study further explained the altitude effect on the tea germplasm distribution in Guizhou,laid the foundation for further study on the evolution and distribution law of tea germplasm resources in Guizhou.

Key words: tea germplasm, population structure, genetic diversity

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