基于分子标记的江西省芝麻地方种质遗传多样性分析

doi:10.3969/j.issn.1004-1524.2021.09.01

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (9): 1565-1580.DOI: 10.3969/j.issn.1004-1524.2021.09.01

基于分子标记的江西省芝麻地方种质遗传多样性分析

王郅琪¹^,²(), 孙建², 梁俊超², 赵云燕¹^,², 颜廷献², 颜小文², 危文亮¹^,^*(), 乐美旺²^,^*()

1.长江大学农学院,湖北荆州 434025
2.江西省农业科学院作物研究所/江西省油料作物生物学重点实验室/国家油料改良中心南昌分中心,江西南昌 330200

收稿日期:2021-05-18 出版日期:2021-09-25 发布日期:2021-10-09
通讯作者: 危文亮,乐美旺
作者简介:乐美旺,E-mail: mwyuecarl@163.com
* 危文亮,E-mail: whwenliang@163.com;
王郅琪(1994—),女,山西朔州人,硕士研究生,研究方向为芝麻种质资源。E-mail: 2609757974@qq.com
基金资助:
国家现代农业产业技术体系(CARS-14);第三次全国农作物种质资源普查与收集行动(111721301354052138)

Study on genetic diversity of sesame germplasm in Jiangxi Province based on molecular markers

WANG Zhiqi¹^,²(), SUN Jian², LIANG Junchao², ZHAO Yunyan¹^,², YAN Tingxian², YAN Xiaowen², WEI Wenliang¹^,^*(), LE Meiwang²^,^*()

1. College of Agriculture, Yangtze University, Jingzhou 434025, China
2. Jiangxi Province Key Laboratory of Oilcrops Biology/Nanchang Branch of National Center of Oilcrops Improvement/Crops Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China

Received:2021-05-18 Online:2021-09-25 Published:2021-10-09
Contact: WEI Wenliang,LE Meiwang

摘要/Abstract

摘要：

为进一步评价“第三次全国农作物种质资源普查与收集行动”中江西收集的132份芝麻地方种质资源的遗传多样性,明确其遗传基础和群体分类特点。利用筛选获得的28对简单重复序列(simple sequence repeat,SSR)引物和26对相关序列扩增多态性(sequence-related amplified polymorphism,SRAP)引物组合对132份江西芝麻地方种质进行遗传多样性研究。结果显示,28对SSR引物共扩增DNA条带265条,其中,多态性条带221条,比例为83.40%,平均每对引物扩增的DNA条带和多态性条带分别为9.46条和7.89条。26对SRAP引物组合扩增得到DNA条带和多态性条带分别为601条和268条,多态性比例为44.59%,每对引物可扩增9~36条DNA条带和2~23条多态性条带,平均每对引物扩增的多态性条带为10.31条。132份种质的遗传相似系数为0.598 2~0.960 7,平均为0.813 5,可见总体的遗传多样性不丰富。6个不同地理区域的芝麻遗传相似系数从北至南呈逐渐下降趋势,赣南区域的地方种质遗传多样性较赣北区域丰富;不同粒色类型比较结果显示,其他粒色芝麻类型(黄色、褐色、黄褐色、红褐色等)的遗传多样性最丰富,其次是白芝麻类型和黑芝麻类型。聚类分析结果表明,来源不同的芝麻种质呈区域性聚集特点,种质间的遗传相似性与地理分布距离有一定的关联,来自赣北和赣东的芝麻种质资源因在亲缘关系上更近而被聚为一类,其余区域聚为一类,这与总体聚类结果基本一致。不同粒色类型中,区域来源不同的种质均相互交错,其聚类结果与地理来源无直接关联。在今后芝麻种质收集工作中,应注重赣南与其他粒色种质的利用,扩展江西乃至我国芝麻品种遗传基础。

关键词: 芝麻, 种质资源, 遗传多样性, 分子标记, 聚类分析

Abstract:

To further evaluate the genetic diversity of 132 sesame germplasm in Jiangxi, which were collected under support of the project of “the Third National Campaign of Crop Germplasm Census and Collection”, and to clarify its genetic basis and population classification characteristics. In this study, 28 simple sequence repeat (SSR) primer pairs and 26 combinations of sequence-related amplified polymorphism (SRAP) primers were used to determine diversity and population classification among 132 sesame genotypes. The results showed that a total of 265 DNA bands were amplified by 28 SSR primer pairs, of which 221 were polymorphic bands, resulting polymorphic ratio of 83.40%, and total DNA bands and polymorphic bands amplified by each primer pair averaged 9.46 and 7.89, respectively. A total of 601 DNA bands and 268 polymorphic bands were amplified by 26 combinations of SRAP primers, resulting polymorphic ratio of 44.59%, and each primer pair amplified 9-36 DNA bands and 2-23 polymorphic bands, the polymorphic bands amplified by each primer pair averaged 10.31. Genetic similarity (GS) of 132 germplasm was between 0.598 2 and 0.960 7, with an average of 0.813 5, which showed that the overall genetic diversity was not abundant. The GS of six different geographic regions showed a gradual decline from north to south of Jiangxi Province. The genetic diversity of local germplasm in southern Jiangxi was more abundant than that in northern Jiangxi. Among different seed coat color groups, the other seed coat colors group (yellow, brown, yellowish-brown, reddish brown, etc) contained the most abundant genetic variation, followed by white group and black group. The results of cluster analysis showed that sesame accessions with similar origin tended to cluster together, indicating a correlationship between genetic similarity and geographical origin of sesame landraces in Jiangxi. Sesame germplasm resources from northern Jiangxi and eastern Jiangxi were grouped into one cluster, which revealed their closer relationship, while the remaining resources from each of the other regions were clustered into one group, which was basically consistent with the overall clustering results. The resources with different origins from three groups divided by seed color were interlaced with each other group, and their clustering results were not directly related to geographic origin. In the future collection of sesame germplasm work, we should pay attention to the utilization of southern Jiangxi germplasm and other seed-color germplasm to expand the genetic basis of sesame varieties in Jiangxi and even in China.

Key words: sesame, germplasm, genetic diversity, molecular marker, cluster analysis

中图分类号:

S565.3

王郅琪, 孙建, 梁俊超, 赵云燕, 颜廷献, 颜小文, 危文亮, 乐美旺. 基于分子标记的江西省芝麻地方种质遗传多样性分析[J]. 浙江农业学报, 2021, 33(9): 1565-1580.

WANG Zhiqi, SUN Jian, LIANG Junchao, ZHAO Yunyan, YAN Tingxian, YAN Xiaowen, WEI Wenliang, LE Meiwang. Study on genetic diversity of sesame germplasm in Jiangxi Province based on molecular markers[J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1565-1580.

图/表 9

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序号	收集编号	名称	收集地点	序号	收集编号	名称	收集地点
Code	Collection number	Name	Origin	Code	Collection number	Name	Origin
1	2017362206	白芝麻 White sesame	都昌县大港镇土目村 Tumu Village, Dagang Town, Duchang County	67	P360622024	本地芝麻 Local sesame	余江区马荃镇林溪村 Linxi Village, Maquan Town, Yujiang District
2	2017362182	黑芝麻 Black sesame	都昌县万户镇新尾村 Xinwei Village, Wanhu Town, Duchang County	68	2019362094	芝麻(黄) Sesame (yellow)	丰城市丽村镇茅头村 Maotou Village, Licun Town, Fengcheng City
3	2017362182	黑芝麻 Black sesame	都昌县万户镇新尾村 Xinwei Village, Wanhu Town, Duchang County	69	2019362027	白芝麻 White sesame	丰城市泉港镇西岸村 Xi’an Village, Quangang Town, Fengcheng City
4	2017363136	南义黑芝麻 Nanyi black sesame	瑞昌市南义镇美景村 Meijing Village, Nanyi Town, Ruichang City	70	2019362071	黑芝麻 Black sesame	丰城市石江乡下山村 Xiashan Village, Shijiang Township, Fengcheng City
5	P360423039	本地芝麻 Local sesame	武宁县石门楼镇西桥村Xiqiao Village, Shimenlou Town, Wuning County	71	2019362072	白芝麻 White sesame	丰城市石江乡下山村 Xiashan Village, Shijiang Township, Fengcheng City
6	2018361011	芝麻(白) White sesame	修水县太阳升镇梅渡村 Meidu Village, Taiyangsheng Town, Xiushui County	72	2019362003	白芝麻 White sesame	丰城市同田乡新联村 Xinlian Village, Tongtian Township, Fengcheng City
7	2018361012	芝麻(黑) Black sesame	修水县太阳升镇梅渡村 Meidu Village, Taiyangsheng Town, Xiushui County	73	P360921006	土黑芝麻 Soil black sesame	奉新县种子管理局 Fengxin County Seed Management Bureau
8	2017361380	芝麻 Sesame	修水县渣津镇西堰村 Xiyan Village, Zhajin Town, Xiushui County	74	P360921015	黄芝麻 Yellow sesame	奉新县种子管理局 Fengxin County Seed Management Bureau
9	2017361460	芝麻 Sesame	永修县立新乡北徐村 Beixu Village, Xinxiang, Yongxiu County	75	P360921007	土白芝麻 Soil white sesame	奉新县种子管理局 Fengxin County Seed Management Bureau
10	2017361047	黑芝麻 Black sesame	永修县三溪桥镇河桥村 Heqiao Village, Sanxiqiao Town, Yongxiu County	76	2018361528	黑芝麻 Black sesame	上高县蒙山镇浒江村 Hujiang Village, Mengshan Town, Shanggao County
11	2017361419	芝麻 Sesame	永修县三溪桥镇横山村 Hengshan Village, Sanxiqiao Town, Yongxiu County	77	P360982027	黑芝麻 Black sesame	樟树市刘公庙镇彭家村 Pengjia Village, Liugongmiao Town, Zhangshu City
12	2017361419	芝麻 Sesame	永修县三溪桥镇横山村 Hengshan Village, Sanxiqiao Town, Yongxiu County	78	P360323004	黑芝麻 Black sesame	芦溪县芦溪镇蔗棚村 Zhepeng Village, Luxi Town, Luxi County
13	2017361028	黑芝麻 Black sesame	永修县三溪桥镇横山村 Hengshan Village, Sanxiqiao Town, Yongxiu County	79	P360323004	黑芝麻 Black sesame	芦溪县芦溪镇蔗棚村 Zhepeng Village, Luxi Town, Luxi County
14	2017361425	芝麻 Sesame	永修县三溪桥镇横山村 Hengshan Village, Sanxiqiao Town, Yongxiu County	80	2017362097	芝麻 Sesame	分宜县钤山镇冶元村 Yeyuan Village, Qianshan Town, Fenyi County
15	2017361425	芝麻 Sesame	永修县三溪桥镇横山村 Hengshan Village, Sanxiqiao Town, Yongxiu County	81	2017362250	黑芝麻 Black sesame	分宜县杨桥镇建陂村 Jianpi Village, Yangqiao Town, Fenyi County
16	2017361015	黑芝麻 Black sesame	永修县吴城镇丁山村 Dingshan Village, Wucheng Town, Yongxiu County	82	2018363368	巴山白芝麻 Bashan white sesame	崇仁县巴山镇何家村 Hejia Village, Bashan Town, Chongren County
17	2017361068	黑芝麻 Black sesame	永修县吴城镇吉山村 Jishan Village, Wucheng Town, Yongxiu County	83	2018363384	马鞍小粒芝麻 Maan small sesame	崇仁县马鞍镇马鞍村 Ma’an Village, Ma’an Town, Chongren County
18	P360281014	本地芝麻 Local sesame	乐平市名口镇戴村 Dai Village, Mingkou Town, Leping City	84	2018363348	孙坊黑芝麻 Sunfang black sesame	崇仁县孙坊镇张坤背村 Zhangkunbei Village, Sunfang Town, Chongren County
19	P361181009	土芝麻 Earth sesame	德兴市绕二镇绕二村 Raoer Village, Raoer Town, Dexing City	85	2018362367	芝麻 Sesame	黎川县宏村镇中湖村 Zhonghu Village, Hongcun Town, Lichuan County
20	P361181027	土芝麻(万村) Earth sesame (Wancun)	德兴市万村乡沙畈村 Shafan Village, Wancun Township, Dexing City	86	2018362560	芝麻 Sesame	黎川县中田乡中田村 Zhongtian Village, Zhongtian Township, Lichuan County
21	2018362740	芝麻(黑) Black sesame	横峰县港边乡余塘村 Yutang Village, Gangbian Township, Hengfeng County	87	P361002026	黑芝麻 Black sesame	临川区湖南乡兰坊村 Lanfang Village, Hunan Township, Linchuan District
22	2019362210	芝麻(白) White sesame	横峰县葛源镇关田村 Guantian Village, Geyuan Town, Hengfeng County	88	2019361048	麻子 Sesame	南城县龙湖镇五角口村 Wujiaokou Village, Longhu Town, Nancheng County
23	2018362669	芝麻(白) White sesame	横峰县葛源镇关田村 Guantian Village, Geyuan Town, Hengfeng County	89	2019361079	白麻 White sesame	南城县龙湖镇小竺村 Xiaozhu Village, Longhu Town, Nancheng County
24	2018362657	芝麻(白) White sesame	横峰县葛源镇石桥村 Shiqiao Village, Geyuan Town, Hengfeng County	90	2019361093	芝麻 Sesame	南城县万坊镇上岭村 Shangling Village, Wanfang Town, Nancheng County
25	2018362724	芝麻(红) Sesame (red)	横峰县莲荷乡义门村 Yimen Village, Lianhe Township, Hengfeng County	91	2019361031	芝麻 Sesame	南城县徐家镇排头村 Paitou Village, Xujia Town, Nancheng County
26	2018362675	芝麻(黑) Sesame (black)	横峰县龙门昄乡坊源村 Fangyuan Village, Longmenban Township, Hengfeng County	92	2019361022	芝麻 Sesame	南城县徐家镇下弓村 Xiagong Village, Xujia Town, Nancheng County
27	2019362216	芝麻(黑) Sesame (black)	横峰县龙门畈乡土岩村 Tuyan Village, Longmenfan Township, Hengfeng County	93	2018362158	白芝麻 White sesame	安福县赤谷乡苍坑村 Cangkeng Village, Chigu Township, Anfu County
28	2019363074	大源白芝麻 Dayuan white sesame	鄱阳县枧田街乡大源村 Dayuan Village, Jiantian Street Township, Poyang County	94	2018362465	白芝麻 White sesame	安福县甘洛乡南阜村 Nanfu Village, Ganluo Township, Anfu County
29	2019363132	莲湖黑芝麻 Lianhu black sesame	鄱阳县莲湖乡向阳村 Xiangyang Village, Lianhu Township, Poyang County	95	2018362102	白芝麻 White sesame	安福县横龙镇利田村 Litian Village, Henglong Town, Anfu County
30	2019363040	饶埠黑芝麻 Raobu black sesame	鄱阳县饶埠镇九甲村 Jiujia Village, Raobu Town, Poyang County	96	2018362137	白芝麻 White sesame	安福县金田乡沿沛村 Yanpei Village, Jintian Township, Anfu County
31	2019363048	饶埠单花黑芝麻 Raobu single flower black sesame	鄱阳县饶埠镇天堂村 Tiantang Village, Raobu Town, Poyang County	97	2018362137	白芝麻 White sesame	安福县金田乡沿沛村 Yanpei Village, Jintian Township, Anfu County
32	2019363114	双港黑芝麻 Shuanggang black sesame	鄱阳县双港镇马鞍山村 Ma’anshan Village, Shuanggang Town, Poyang County	98	2018362176	白芝麻 White sesame	安福县严田镇坛洲村 Tanzhou Village, Yantian Town, Anfu County
33	2019363002	银宝湖黑芝麻 Yinbao lake black sesame	鄱阳县银宝湖乡和平村 Heping Village, Yinbaohu Township, Poyang County	99	P360802010	白芝麻 White sesame	吉州区曲濑镇卢家洲 Lujiazhou, Qulai Town, Jizhou District
34	2019363020	银宝湖西分芝麻 Yinbao lake xifen sesame	鄱阳县银宝湖乡西分村 Xifen Village, Yinbaohu Township, Poyang County	100	P360802011	红芝麻 Red sesame	吉州区曲濑镇卢家洲 Lujiazhou, Qulai Town, Jizhou District
35	P361124066	汪二红芝麻 Wang Erhong sesame	铅山县 Qianshan County	101	P360802009	黑芝麻 Black sesame	吉州区曲濑镇卢家洲 Lujiazhou, Qulai Town, Jizhou District
36	2018363625	大源黑芝麻 Dayuan black sesame	万年县大源镇界福村 Jiefu Village, Dayuan Town, Wannian County	102	2018363519	东上白芝麻 Dongshang white sesame	井冈山市东上乡东上村 Dongshang Village, Dongshang Township, Jinggangshan City
37	2018363635	黑芝麻 Black sesame	万年县大源镇界福村 Jiefu Village, Dayuan Town, Wannian County	103	2018363519	东上白芝麻 Dongshang white sesame	井冈山市东上乡东上村 Dongshang Village, Dongshang Township, Jinggangshan City
38	2018363629	大源四棱黑芝麻 Dayuan four-edged black sesame	万年县大源镇界福村 Jiefu Village, Dayuan Town, Wannian County	104	2018363567	芝麻 Sesame	井冈山市新城镇排头村 Paitou Village, Xincheng Town, Jinggangshan City
39	2018363701	苏桥白芝麻 Suqiao white sesame	万年县苏桥乡楼下村 Louxia Village, Suqiao Township, Wannian County	105	2018363476	长古岭芝麻 Changguling sesame	井冈山市长古岭林场湘洲村 Xiangzhou Village, Changguling Forest Farm, Jinggangshan City
40	2018363710	麻畲黑芝麻 Moshe black sesame	万年县苏桥乡麻畲村 Mashe Village, Suqiao Township, Wannian County	106	P360803037	芝麻 Sesame	青原区河东街道芜下村 Wuxia Village, Hedong Street, Qingyuan District
41	P361130031	本地芝麻 Local sesame	婺源县秋口镇樟槎村 Zhangcha Village, Qiukou Town, Wuyuan County	107	P360828012	芝麻 Sesame	万安县弹前乡店头 Diantou Village, Tanqian Township, Wan’an County
42	P361130031	本地芝麻 Local sesame	婺源县秋口镇樟槎村 Zhangcha Village, Qiukou Town, Wuyuan County	108	2018361088	黑芝麻 Black sesame	峡江县金江乡金滩村 Jintan Village, Jinjiang Township, Xiajiang County
43	P361130009	本地芝麻 Local sesame	婺源县蚺城街道办香田村 Xiangtian Village, Wucheng Sub- district Office, Wuyuan County	109	2018361089	白芝麻 White sesame	峡江县金江乡金滩村 Jintan Village, Jinjiang Township, Xiajiang County
44	P361130066	太白黑芝麻 Taibai black sesame	婺源县太白乡新屋村 Xinwu Village, Taibai Township, Wuyuan County	110	2018361711	黑芝麻 Black sesame	峡江县金江乡南湖村 Nanhu Village, Jinjiang Township, Xiajiang County
45	P361130079	太白红芝麻 Taibai red sesame	婺源县沱川乡小沱村 Xiaotuo Village, Tuochuan Township, Wuyuan County	111	2018361077	麻子 Sesame	峡江县金江乡新溪村 Xinxi Village, Jinjiang Township, Xiajiang County
46	P361130060	浙源芝麻 Zheyuan sesame	婺源县浙源乡凤山村 Fengshan Village, Zheyuan Township, Wuyuan County	112	2018361127	黑芝麻 Black sesame	峡江县马埠镇曾安村 Zeng’an Village, Mabu Town, Xiajiang County
47	P361126031	芝麻 Sesame	弋阳县 Yiyang County	113	2018361118	黑芝麻 Black sesame	峡江县马埠镇夏塘村 Xiatang Village, Mabu Town, Xiajiang County
48	2018361410	芝麻 Sesame	玉山县怀玉乡洋塘村 Yangtang Village, Huaiyu Township, Yushan County	114	2018361119	白芝麻 White sesame	峡江县马埠镇夏塘村 Xiatang Village, Mabu Town, Xiajiang County
49	2018361424	油麻 Oil sesame	玉山县怀玉乡玉峰村 Yufeng Village, Huaiyu Township, Yushan County	115	2018361755	红芝麻 Red sesame	峡江县仁和镇官田村 Guantian Village, Renhe Town, Xiajiang County
50	2018361490	油麻 Oil sesame	玉山县双明镇陶源村 Taoyuan Village, Shuangming Town, Yushan County	116	2018361756	白芝麻 White sesame	峡江县仁和镇官田村 Guantian Village, Renhe Town, Xiajiang County
51	2018361447	黑芝麻 Black sesame	玉山县紫湖镇程村村 Chengcun Village, Zihu Town, Yushan County	117	2018361097	芝麻 Sesame	峡江县仁和镇官田村 Guantian Village, Renhe Town, Xiajiang County
52	2018361448	高杆芝麻 Tall sesame	玉山县紫湖镇程村村 Chengcun Village, Zihu Town, Yushan County	118	2018361252	芝麻 Sesame	定南县岭北镇南丰村 Nanfeng Village, Lingbei Town, Dingnan County
53	2018361449	矮杆芝麻 Dwarf sesame	玉山县紫湖镇程村村 Chengcun Village, Zihu Town, Yushan County	119	2018361673	芝麻 Sesame	定南县天九镇东山村 Dongshan Village, Tianjiu Town, Dingnan County
54	P360124012	进贤县黑芝麻 Jinxian County black sesame	进贤县南台乡赤岭林家村 Chiling Linjia Village, Nantai Township, Jinxian County	120	2018362048	黑芝麻 Black sesame	宁都县黄石镇大岭村 Daling Village, Huangshi Town, Ningdu County
55	P360124032	本地红芝麻 Local red sesame	进贤县钟陵乡三岸村 San’an Village, Zhongling Township, Jinxian County	121	P360729025	芝麻 Sesame	全南县城厢镇黄埠村 Huangbu Village, Chengxiang Town, Quannan County
56	P360124033	本地白芝麻 Local white sesame	进贤县钟陵乡三岸村 San’an Village, Zhongling Township, Jinxian County	122	2018363028	万田白芝麻 Mantian white sesame	瑞金市万田乡井垅村 Jinglong Village, Wantian Township, Ruijin City
57	2019361305	黑芝麻 Black sesame	余江区画桥镇大桥村 Daqiao Village, Huaqiao Town, Yujiang District	123	2018363080	叶坪黑芝麻 Yeping black sesame	瑞金市叶坪乡山岐村 Shanqi Village, Yeping Township, Ruijin City
58	2019361312	红芝麻 Red sesame	余江区画桥镇基墩村 Jidun Village, Huaqiao Town, Yujiang District	124	2018362614	白芝麻 White sesame	兴国县鼎龙乡湖溪村 Huxi Village, Dinglong Township, Xingguo County
59	2019361318	黑芝麻 Black sesame	余江区黄庄乡兰岭村 Lanling Village, Huangzhuang Township, Yujiang District	125	2018362297	黑芝麻 Black sesame	兴国县鼎龙乡灵山村 Lingshan Village, Dinglong Township, Xingguo County
60	2019361371	黑芝麻 Black sesame	余江区潢溪镇坪上村 Pingshang Village, Huangxi Town, Yujiang District	126	2018362642	白芝麻 White sesame	兴国县良村镇前村村 Qiancun Village, Liangcun Town, Xingguo County
61	2019361359	黑芝麻 Black sesame	余江区马荃镇管场村 Guanchang Village, Maquan Town, Yujiang District	127	2018362577	白芝麻 White sesame	兴国县社富乡九山村 Jiushan Village, Shefu Township, Xingguo County
62	2019361341	黑芝麻 Black sesame	余江区马荃镇岩前村 Yanqian Village, Maquan Town, Yujiang District	128	2018363238	项山白芝麻 Xiangshan white sesame	寻乌县 Xunwu County
63	2018361341	黑芝麻 Black sesame	余江区画桥镇画桥村 Huaqiao Village, Huaqiao Town, Yujiang District	129	2018363200	水源白芝麻S Huiyuan white sesame	寻乌县水源乡龙塘村 Longtang Village, Shuiyuan Township, Xunwu County
64	2018361301	黑芝麻 Black sesame	余江区马荃镇汉溪村 Hanxi Village, Maquan Town, Yujiang District	130	2019361410	黑麻子 Black sesame	上高县蒙山镇菱湖村 Linghu Village, Mengshan Town, Shanggao County
65	2018361320	黑芝麻 Black sesame	余江区马荃镇岩前村 Yanqian Village, Maquan Town, Yujiang District	131	2019363426	三都白芝麻 Sandu white sesame	铜鼓县三都镇西向村 Xixiang Village, Sandu Town, Tonggu County
66	2018361360	黑芝麻 Black sesame	余江区杨溪乡新危村 Xinwei Village, Yangxi Township, Yujiang District	132	2019361643	白芝麻 White sesame	南城县上塘镇余公斗村 Yugongdou Village, Shangtang Town, Nancheng County

序号	收集编号	名称	收集地点	序号	收集编号	名称	收集地点
Code	Collection number	Name	Origin	Code	Collection number	Name	Origin
1	2017362206	白芝麻 White sesame	都昌县大港镇土目村 Tumu Village, Dagang Town, Duchang County	67	P360622024	本地芝麻 Local sesame	余江区马荃镇林溪村 Linxi Village, Maquan Town, Yujiang District
2	2017362182	黑芝麻 Black sesame	都昌县万户镇新尾村 Xinwei Village, Wanhu Town, Duchang County	68	2019362094	芝麻(黄) Sesame (yellow)	丰城市丽村镇茅头村 Maotou Village, Licun Town, Fengcheng City
3	2017362182	黑芝麻 Black sesame	都昌县万户镇新尾村 Xinwei Village, Wanhu Town, Duchang County	69	2019362027	白芝麻 White sesame	丰城市泉港镇西岸村 Xi’an Village, Quangang Town, Fengcheng City
4	2017363136	南义黑芝麻 Nanyi black sesame	瑞昌市南义镇美景村 Meijing Village, Nanyi Town, Ruichang City	70	2019362071	黑芝麻 Black sesame	丰城市石江乡下山村 Xiashan Village, Shijiang Township, Fengcheng City
5	P360423039	本地芝麻 Local sesame	武宁县石门楼镇西桥村Xiqiao Village, Shimenlou Town, Wuning County	71	2019362072	白芝麻 White sesame	丰城市石江乡下山村 Xiashan Village, Shijiang Township, Fengcheng City
6	2018361011	芝麻(白) White sesame	修水县太阳升镇梅渡村 Meidu Village, Taiyangsheng Town, Xiushui County	72	2019362003	白芝麻 White sesame	丰城市同田乡新联村 Xinlian Village, Tongtian Township, Fengcheng City
7	2018361012	芝麻(黑) Black sesame	修水县太阳升镇梅渡村 Meidu Village, Taiyangsheng Town, Xiushui County	73	P360921006	土黑芝麻 Soil black sesame	奉新县种子管理局 Fengxin County Seed Management Bureau
8	2017361380	芝麻 Sesame	修水县渣津镇西堰村 Xiyan Village, Zhajin Town, Xiushui County	74	P360921015	黄芝麻 Yellow sesame	奉新县种子管理局 Fengxin County Seed Management Bureau
9	2017361460	芝麻 Sesame	永修县立新乡北徐村 Beixu Village, Xinxiang, Yongxiu County	75	P360921007	土白芝麻 Soil white sesame	奉新县种子管理局 Fengxin County Seed Management Bureau
10	2017361047	黑芝麻 Black sesame	永修县三溪桥镇河桥村 Heqiao Village, Sanxiqiao Town, Yongxiu County	76	2018361528	黑芝麻 Black sesame	上高县蒙山镇浒江村 Hujiang Village, Mengshan Town, Shanggao County
11	2017361419	芝麻 Sesame	永修县三溪桥镇横山村 Hengshan Village, Sanxiqiao Town, Yongxiu County	77	P360982027	黑芝麻 Black sesame	樟树市刘公庙镇彭家村 Pengjia Village, Liugongmiao Town, Zhangshu City
12	2017361419	芝麻 Sesame	永修县三溪桥镇横山村 Hengshan Village, Sanxiqiao Town, Yongxiu County	78	P360323004	黑芝麻 Black sesame	芦溪县芦溪镇蔗棚村 Zhepeng Village, Luxi Town, Luxi County
13	2017361028	黑芝麻 Black sesame	永修县三溪桥镇横山村 Hengshan Village, Sanxiqiao Town, Yongxiu County	79	P360323004	黑芝麻 Black sesame	芦溪县芦溪镇蔗棚村 Zhepeng Village, Luxi Town, Luxi County
14	2017361425	芝麻 Sesame	永修县三溪桥镇横山村 Hengshan Village, Sanxiqiao Town, Yongxiu County	80	2017362097	芝麻 Sesame	分宜县钤山镇冶元村 Yeyuan Village, Qianshan Town, Fenyi County
15	2017361425	芝麻 Sesame	永修县三溪桥镇横山村 Hengshan Village, Sanxiqiao Town, Yongxiu County	81	2017362250	黑芝麻 Black sesame	分宜县杨桥镇建陂村 Jianpi Village, Yangqiao Town, Fenyi County
16	2017361015	黑芝麻 Black sesame	永修县吴城镇丁山村 Dingshan Village, Wucheng Town, Yongxiu County	82	2018363368	巴山白芝麻 Bashan white sesame	崇仁县巴山镇何家村 Hejia Village, Bashan Town, Chongren County
17	2017361068	黑芝麻 Black sesame	永修县吴城镇吉山村 Jishan Village, Wucheng Town, Yongxiu County	83	2018363384	马鞍小粒芝麻 Maan small sesame	崇仁县马鞍镇马鞍村 Ma’an Village, Ma’an Town, Chongren County
18	P360281014	本地芝麻 Local sesame	乐平市名口镇戴村 Dai Village, Mingkou Town, Leping City	84	2018363348	孙坊黑芝麻 Sunfang black sesame	崇仁县孙坊镇张坤背村 Zhangkunbei Village, Sunfang Town, Chongren County
19	P361181009	土芝麻 Earth sesame	德兴市绕二镇绕二村 Raoer Village, Raoer Town, Dexing City	85	2018362367	芝麻 Sesame	黎川县宏村镇中湖村 Zhonghu Village, Hongcun Town, Lichuan County
20	P361181027	土芝麻(万村) Earth sesame (Wancun)	德兴市万村乡沙畈村 Shafan Village, Wancun Township, Dexing City	86	2018362560	芝麻 Sesame	黎川县中田乡中田村 Zhongtian Village, Zhongtian Township, Lichuan County
21	2018362740	芝麻(黑) Black sesame	横峰县港边乡余塘村 Yutang Village, Gangbian Township, Hengfeng County	87	P361002026	黑芝麻 Black sesame	临川区湖南乡兰坊村 Lanfang Village, Hunan Township, Linchuan District
22	2019362210	芝麻(白) White sesame	横峰县葛源镇关田村 Guantian Village, Geyuan Town, Hengfeng County	88	2019361048	麻子 Sesame	南城县龙湖镇五角口村 Wujiaokou Village, Longhu Town, Nancheng County
23	2018362669	芝麻(白) White sesame	横峰县葛源镇关田村 Guantian Village, Geyuan Town, Hengfeng County	89	2019361079	白麻 White sesame	南城县龙湖镇小竺村 Xiaozhu Village, Longhu Town, Nancheng County
24	2018362657	芝麻(白) White sesame	横峰县葛源镇石桥村 Shiqiao Village, Geyuan Town, Hengfeng County	90	2019361093	芝麻 Sesame	南城县万坊镇上岭村 Shangling Village, Wanfang Town, Nancheng County
25	2018362724	芝麻(红) Sesame (red)	横峰县莲荷乡义门村 Yimen Village, Lianhe Township, Hengfeng County	91	2019361031	芝麻 Sesame	南城县徐家镇排头村 Paitou Village, Xujia Town, Nancheng County
26	2018362675	芝麻(黑) Sesame (black)	横峰县龙门昄乡坊源村 Fangyuan Village, Longmenban Township, Hengfeng County	92	2019361022	芝麻 Sesame	南城县徐家镇下弓村 Xiagong Village, Xujia Town, Nancheng County
27	2019362216	芝麻(黑) Sesame (black)	横峰县龙门畈乡土岩村 Tuyan Village, Longmenfan Township, Hengfeng County	93	2018362158	白芝麻 White sesame	安福县赤谷乡苍坑村 Cangkeng Village, Chigu Township, Anfu County
28	2019363074	大源白芝麻 Dayuan white sesame	鄱阳县枧田街乡大源村 Dayuan Village, Jiantian Street Township, Poyang County	94	2018362465	白芝麻 White sesame	安福县甘洛乡南阜村 Nanfu Village, Ganluo Township, Anfu County
29	2019363132	莲湖黑芝麻 Lianhu black sesame	鄱阳县莲湖乡向阳村 Xiangyang Village, Lianhu Township, Poyang County	95	2018362102	白芝麻 White sesame	安福县横龙镇利田村 Litian Village, Henglong Town, Anfu County
30	2019363040	饶埠黑芝麻 Raobu black sesame	鄱阳县饶埠镇九甲村 Jiujia Village, Raobu Town, Poyang County	96	2018362137	白芝麻 White sesame	安福县金田乡沿沛村 Yanpei Village, Jintian Township, Anfu County
31	2019363048	饶埠单花黑芝麻 Raobu single flower black sesame	鄱阳县饶埠镇天堂村 Tiantang Village, Raobu Town, Poyang County	97	2018362137	白芝麻 White sesame	安福县金田乡沿沛村 Yanpei Village, Jintian Township, Anfu County
32	2019363114	双港黑芝麻 Shuanggang black sesame	鄱阳县双港镇马鞍山村 Ma’anshan Village, Shuanggang Town, Poyang County	98	2018362176	白芝麻 White sesame	安福县严田镇坛洲村 Tanzhou Village, Yantian Town, Anfu County
33	2019363002	银宝湖黑芝麻 Yinbao lake black sesame	鄱阳县银宝湖乡和平村 Heping Village, Yinbaohu Township, Poyang County	99	P360802010	白芝麻 White sesame	吉州区曲濑镇卢家洲 Lujiazhou, Qulai Town, Jizhou District
34	2019363020	银宝湖西分芝麻 Yinbao lake xifen sesame	鄱阳县银宝湖乡西分村 Xifen Village, Yinbaohu Township, Poyang County	100	P360802011	红芝麻 Red sesame	吉州区曲濑镇卢家洲 Lujiazhou, Qulai Town, Jizhou District
35	P361124066	汪二红芝麻 Wang Erhong sesame	铅山县 Qianshan County	101	P360802009	黑芝麻 Black sesame	吉州区曲濑镇卢家洲 Lujiazhou, Qulai Town, Jizhou District
36	2018363625	大源黑芝麻 Dayuan black sesame	万年县大源镇界福村 Jiefu Village, Dayuan Town, Wannian County	102	2018363519	东上白芝麻 Dongshang white sesame	井冈山市东上乡东上村 Dongshang Village, Dongshang Township, Jinggangshan City
37	2018363635	黑芝麻 Black sesame	万年县大源镇界福村 Jiefu Village, Dayuan Town, Wannian County	103	2018363519	东上白芝麻 Dongshang white sesame	井冈山市东上乡东上村 Dongshang Village, Dongshang Township, Jinggangshan City
38	2018363629	大源四棱黑芝麻 Dayuan four-edged black sesame	万年县大源镇界福村 Jiefu Village, Dayuan Town, Wannian County	104	2018363567	芝麻 Sesame	井冈山市新城镇排头村 Paitou Village, Xincheng Town, Jinggangshan City
39	2018363701	苏桥白芝麻 Suqiao white sesame	万年县苏桥乡楼下村 Louxia Village, Suqiao Township, Wannian County	105	2018363476	长古岭芝麻 Changguling sesame	井冈山市长古岭林场湘洲村 Xiangzhou Village, Changguling Forest Farm, Jinggangshan City
40	2018363710	麻畲黑芝麻 Moshe black sesame	万年县苏桥乡麻畲村 Mashe Village, Suqiao Township, Wannian County	106	P360803037	芝麻 Sesame	青原区河东街道芜下村 Wuxia Village, Hedong Street, Qingyuan District
41	P361130031	本地芝麻 Local sesame	婺源县秋口镇樟槎村 Zhangcha Village, Qiukou Town, Wuyuan County	107	P360828012	芝麻 Sesame	万安县弹前乡店头 Diantou Village, Tanqian Township, Wan’an County
42	P361130031	本地芝麻 Local sesame	婺源县秋口镇樟槎村 Zhangcha Village, Qiukou Town, Wuyuan County	108	2018361088	黑芝麻 Black sesame	峡江县金江乡金滩村 Jintan Village, Jinjiang Township, Xiajiang County
43	P361130009	本地芝麻 Local sesame	婺源县蚺城街道办香田村 Xiangtian Village, Wucheng Sub- district Office, Wuyuan County	109	2018361089	白芝麻 White sesame	峡江县金江乡金滩村 Jintan Village, Jinjiang Township, Xiajiang County
44	P361130066	太白黑芝麻 Taibai black sesame	婺源县太白乡新屋村 Xinwu Village, Taibai Township, Wuyuan County	110	2018361711	黑芝麻 Black sesame	峡江县金江乡南湖村 Nanhu Village, Jinjiang Township, Xiajiang County
45	P361130079	太白红芝麻 Taibai red sesame	婺源县沱川乡小沱村 Xiaotuo Village, Tuochuan Township, Wuyuan County	111	2018361077	麻子 Sesame	峡江县金江乡新溪村 Xinxi Village, Jinjiang Township, Xiajiang County
46	P361130060	浙源芝麻 Zheyuan sesame	婺源县浙源乡凤山村 Fengshan Village, Zheyuan Township, Wuyuan County	112	2018361127	黑芝麻 Black sesame	峡江县马埠镇曾安村 Zeng’an Village, Mabu Town, Xiajiang County
47	P361126031	芝麻 Sesame	弋阳县 Yiyang County	113	2018361118	黑芝麻 Black sesame	峡江县马埠镇夏塘村 Xiatang Village, Mabu Town, Xiajiang County
48	2018361410	芝麻 Sesame	玉山县怀玉乡洋塘村 Yangtang Village, Huaiyu Township, Yushan County	114	2018361119	白芝麻 White sesame	峡江县马埠镇夏塘村 Xiatang Village, Mabu Town, Xiajiang County
49	2018361424	油麻 Oil sesame	玉山县怀玉乡玉峰村 Yufeng Village, Huaiyu Township, Yushan County	115	2018361755	红芝麻 Red sesame	峡江县仁和镇官田村 Guantian Village, Renhe Town, Xiajiang County
50	2018361490	油麻 Oil sesame	玉山县双明镇陶源村 Taoyuan Village, Shuangming Town, Yushan County	116	2018361756	白芝麻 White sesame	峡江县仁和镇官田村 Guantian Village, Renhe Town, Xiajiang County
51	2018361447	黑芝麻 Black sesame	玉山县紫湖镇程村村 Chengcun Village, Zihu Town, Yushan County	117	2018361097	芝麻 Sesame	峡江县仁和镇官田村 Guantian Village, Renhe Town, Xiajiang County
52	2018361448	高杆芝麻 Tall sesame	玉山县紫湖镇程村村 Chengcun Village, Zihu Town, Yushan County	118	2018361252	芝麻 Sesame	定南县岭北镇南丰村 Nanfeng Village, Lingbei Town, Dingnan County
53	2018361449	矮杆芝麻 Dwarf sesame	玉山县紫湖镇程村村 Chengcun Village, Zihu Town, Yushan County	119	2018361673	芝麻 Sesame	定南县天九镇东山村 Dongshan Village, Tianjiu Town, Dingnan County
54	P360124012	进贤县黑芝麻 Jinxian County black sesame	进贤县南台乡赤岭林家村 Chiling Linjia Village, Nantai Township, Jinxian County	120	2018362048	黑芝麻 Black sesame	宁都县黄石镇大岭村 Daling Village, Huangshi Town, Ningdu County
55	P360124032	本地红芝麻 Local red sesame	进贤县钟陵乡三岸村 San’an Village, Zhongling Township, Jinxian County	121	P360729025	芝麻 Sesame	全南县城厢镇黄埠村 Huangbu Village, Chengxiang Town, Quannan County
56	P360124033	本地白芝麻 Local white sesame	进贤县钟陵乡三岸村 San’an Village, Zhongling Township, Jinxian County	122	2018363028	万田白芝麻 Mantian white sesame	瑞金市万田乡井垅村 Jinglong Village, Wantian Township, Ruijin City
57	2019361305	黑芝麻 Black sesame	余江区画桥镇大桥村 Daqiao Village, Huaqiao Town, Yujiang District	123	2018363080	叶坪黑芝麻 Yeping black sesame	瑞金市叶坪乡山岐村 Shanqi Village, Yeping Township, Ruijin City
58	2019361312	红芝麻 Red sesame	余江区画桥镇基墩村 Jidun Village, Huaqiao Town, Yujiang District	124	2018362614	白芝麻 White sesame	兴国县鼎龙乡湖溪村 Huxi Village, Dinglong Township, Xingguo County
59	2019361318	黑芝麻 Black sesame	余江区黄庄乡兰岭村 Lanling Village, Huangzhuang Township, Yujiang District	125	2018362297	黑芝麻 Black sesame	兴国县鼎龙乡灵山村 Lingshan Village, Dinglong Township, Xingguo County
60	2019361371	黑芝麻 Black sesame	余江区潢溪镇坪上村 Pingshang Village, Huangxi Town, Yujiang District	126	2018362642	白芝麻 White sesame	兴国县良村镇前村村 Qiancun Village, Liangcun Town, Xingguo County
61	2019361359	黑芝麻 Black sesame	余江区马荃镇管场村 Guanchang Village, Maquan Town, Yujiang District	127	2018362577	白芝麻 White sesame	兴国县社富乡九山村 Jiushan Village, Shefu Township, Xingguo County
62	2019361341	黑芝麻 Black sesame	余江区马荃镇岩前村 Yanqian Village, Maquan Town, Yujiang District	128	2018363238	项山白芝麻 Xiangshan white sesame	寻乌县 Xunwu County
63	2018361341	黑芝麻 Black sesame	余江区画桥镇画桥村 Huaqiao Village, Huaqiao Town, Yujiang District	129	2018363200	水源白芝麻S Huiyuan white sesame	寻乌县水源乡龙塘村 Longtang Village, Shuiyuan Township, Xunwu County
64	2018361301	黑芝麻 Black sesame	余江区马荃镇汉溪村 Hanxi Village, Maquan Town, Yujiang District	130	2019361410	黑麻子 Black sesame	上高县蒙山镇菱湖村 Linghu Village, Mengshan Town, Shanggao County
65	2018361320	黑芝麻 Black sesame	余江区马荃镇岩前村 Yanqian Village, Maquan Town, Yujiang District	131	2019363426	三都白芝麻 Sandu white sesame	铜鼓县三都镇西向村 Xixiang Village, Sandu Town, Tonggu County
66	2018361360	黑芝麻 Black sesame	余江区杨溪乡新危村 Xinwei Village, Yangxi Township, Yujiang District	132	2019361643	白芝麻 White sesame	南城县上塘镇余公斗村 Yugongdou Village, Shangtang Town, Nancheng County

引物名称	正向引物序列	反向引物序列
Primer names	Forward primer sequence (5’→3’)	Reverse primer sequence (5’→3’)
HS205	GATGTGATGGTGGTGAGAGC	GCTATGCGTTGAATGAAGAC
ZM22	GCTATGCGTTGAATGAAGAC	CCACTGCACACTACAGTTTTT
ZM43	CTTGGATATCAGTTTCCTGTG	GTTCTCCACAGTCAAAACACT
HS183	TTGAAGGCGATGTAGGTCAG	CTGGTGATTCAGCCATTTTG
HS184	CTGGTGATTCAGCCATTTTG	TTGAAGGCGATGTAGGTCAG
HS259	AAAGCCTCCCATACGATCAC	ACCGACGGAAACAACTAAGC
HS270	TGCCCATGGATTCAATTTTT	CAGAGGTCACCATTGACGAG
HS62	ATGATCATGACGACCCTCAA	ATAGCTGGGCAGGCCTTTAT
HS63	CCAATCCTCCTCCATGTTTT	AGGAGAACTCCCCAGCAGTA
HS123	CTTCTCCTTTCATTCCCGAG	CAGGAAACCACAACGAAATG
HS94	CATGTGTTCTCTCCCACCAC	TCTTGACCATGTTTTCCACC
HS02	CCATTAAATTCTTGCTCCCC	CTGGTCGTATGCAGCATCTT
HS198	CGTCATGGTCTCCCCAAA	CCTGCTAATCAACACCGAAA
HS294	TTTCCCTTTCTCCTCCTTCA	TTTGTCCCCTCCGATAAGAC
HS220	TCATGCCACCTCTCTTTCAG	GAGGGGAGGGACAAGTATCA
HS225	TACTCCTTCCAACCTCACCC	ACCCATGAGCATGAGCATAA
HS22	AGCCCACGAAACTCTTCCTA	GTTCGAGCCCTTCAAGATTC
HS33	ATGGGGGAAGGAATAGGATT	CATCTCGCATCAAACTTGC
HS50	GATGGGTTCTTTATGGGGTG	AGAAAAAGCAAAAGGGAGGG
HS53	GAAGCTTGAAGAGAGGAGGG	ATGGAACTTCTCCGATCACC
ZM08	TCTCTCTCTCTCTCGTTCTTG	CCCACTGTACCTCTCCATATT
ZM43	CTTGGATATCAGTTTCCTGTG	GTTCTCCACAGTCAAAACACT
ZHY23	ATGGGCGTATCAGTTTCGAC	TTTCCTGCCAACTTTTCTGG
ZHY24	GGGGCACAGAGTGGATGTAG	GGACCATGTAATCCCAGCAC
ZHY25	CAGCCCCTTCTTCTTCCTTC	CAGCTGGCAGATCAGTATGG
Sa72	GCAGCAGTTCCGTTCTTG	AGTGCTGAATTTAGTCTGCATAG
Sa108	CCACTCAAAATTTTCACTAAGAA	TCGTCTTCCTCTCTCCCC
Sa182	CCATTGAAAACTGCACACAA	TCCACACACAGAGAGCCC

引物名称	正向引物序列	反向引物序列
Primer names	Forward primer sequence (5’→3’)	Reverse primer sequence (5’→3’)
HS205	GATGTGATGGTGGTGAGAGC	GCTATGCGTTGAATGAAGAC
ZM22	GCTATGCGTTGAATGAAGAC	CCACTGCACACTACAGTTTTT
ZM43	CTTGGATATCAGTTTCCTGTG	GTTCTCCACAGTCAAAACACT
HS183	TTGAAGGCGATGTAGGTCAG	CTGGTGATTCAGCCATTTTG
HS184	CTGGTGATTCAGCCATTTTG	TTGAAGGCGATGTAGGTCAG
HS259	AAAGCCTCCCATACGATCAC	ACCGACGGAAACAACTAAGC
HS270	TGCCCATGGATTCAATTTTT	CAGAGGTCACCATTGACGAG
HS62	ATGATCATGACGACCCTCAA	ATAGCTGGGCAGGCCTTTAT
HS63	CCAATCCTCCTCCATGTTTT	AGGAGAACTCCCCAGCAGTA
HS123	CTTCTCCTTTCATTCCCGAG	CAGGAAACCACAACGAAATG
HS94	CATGTGTTCTCTCCCACCAC	TCTTGACCATGTTTTCCACC
HS02	CCATTAAATTCTTGCTCCCC	CTGGTCGTATGCAGCATCTT
HS198	CGTCATGGTCTCCCCAAA	CCTGCTAATCAACACCGAAA
HS294	TTTCCCTTTCTCCTCCTTCA	TTTGTCCCCTCCGATAAGAC
HS220	TCATGCCACCTCTCTTTCAG	GAGGGGAGGGACAAGTATCA
HS225	TACTCCTTCCAACCTCACCC	ACCCATGAGCATGAGCATAA
HS22	AGCCCACGAAACTCTTCCTA	GTTCGAGCCCTTCAAGATTC
HS33	ATGGGGGAAGGAATAGGATT	CATCTCGCATCAAACTTGC
HS50	GATGGGTTCTTTATGGGGTG	AGAAAAAGCAAAAGGGAGGG
HS53	GAAGCTTGAAGAGAGGAGGG	ATGGAACTTCTCCGATCACC
ZM08	TCTCTCTCTCTCTCGTTCTTG	CCCACTGTACCTCTCCATATT
ZM43	CTTGGATATCAGTTTCCTGTG	GTTCTCCACAGTCAAAACACT
ZHY23	ATGGGCGTATCAGTTTCGAC	TTTCCTGCCAACTTTTCTGG
ZHY24	GGGGCACAGAGTGGATGTAG	GGACCATGTAATCCCAGCAC
ZHY25	CAGCCCCTTCTTCTTCCTTC	CAGCTGGCAGATCAGTATGG
Sa72	GCAGCAGTTCCGTTCTTG	AGTGCTGAATTTAGTCTGCATAG
Sa108	CCACTCAAAATTTTCACTAAGAA	TCGTCTTCCTCTCTCCCC
Sa182	CCATTGAAAACTGCACACAA	TCCACACACAGAGAGCCC

编号	反向引物序列	编号	正向引物序列
Code	Revers primer sequence (5’→3’)	Code	Forward primer sequence (5’→3’)
Em01	GACTGCGTACGAATTAAT	Me01	TGAGTCCAAACCGGATA
Em02	GACTGCGTACGAATTTGC	Me02	TGAGTCCAAACCGGAGC
Em03	GACTGCGTACGAATTGAC	Me03	TGAGTCCAAACCGGAAT
Em04	GACTGCGTACGAATTTGA	Me04	TGAGTCCAAACCGGACC
Em05	GACTGCGTACGAATTAAC	Me07	TGAGTCCAAACCGGTCC
Em07	GACTGCGTACGAATTCAA	Me13	TGAGTCCAAACCGGATG
Em08	GACTGCGTACGAATTCTG	Me14	TGAGTCCAAACCGGAGA
Em09	GACTGCGTACGAATTCGA	Me19	TGAGTCCAAACCGGCAG
Em10	GACTGCGTACGAATTCAG	Me21	TGAGTCCAAACCGGCTT
Em11	GACTGCGTACGAATTCCA
Em12	GACTGCGTACGAATTAAG

基于分子标记的江西省芝麻地方种质遗传多样性分析

Study on genetic diversity of sesame germplasm in Jiangxi Province based on molecular markers

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Metrics

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SSR引物 SSR primer	总带数量 Total Bands	多态性带 Polymorphic bands	多态性比例 Percentage of polymorphic bands/%	SRAP引物 SRAP primer	总带数量 Total Bands	多态性带 Polymorphic bands	多态性比例 Percentage of polymorphic bands/%
HS02	6	6	100.00	Em1/Me1	25	17	68.00
HS06	6	4	66.67	Em1/Me21	29	13	44.83
HS22	6	6	100.00	Em2/Me19	20	7	35.00
HS33	8	6	75.00	Em3/Me3	27	15	55.56
HS50	11	3	27.27	Em3/Me13	31	19	61.29
HS53	7	7	100.00	Em3/Me21	26	12	46.15
HS62	16	16	100.00	Em4/Me1	9	2	22.22
HS63	7	6	85.71	Em4/Me13	26	11	42.31
HS94	15	14	93.33	Em4/Me14	15	12	80.00
HS123	11	10	90.91	Em4/Me19	28	20	71.43
HS137	8	6	75.00	Em4/Me21	33	11	33.33
HS183	8	8	100.00	Em7/Me2	19	2	10.53
HS184	9	8	88.89	Em7/Me3	36	23	63.89
HS198	11	11	100.00	Em7/Me14	16	4	25.00
HS205	9	9	100.00	Em7/Me19	30	9	30.00
HS220	21	21	100.00	Em7/Me21	18	4	22.22
HS225	7	7	100.00	Em8/Me2	21	7	33.33
HS259	11	3	27.27	Em8/Me19	20	10	50.00
HS270	16	10	62.50	Em9/Me14	13	6	46.15
HS294	8	8	100.00	Em10/Me19	27	13	48.15
ZM08	11	11	100.00	Em11/Me14	25	9	36.00
ZM22	5	5	100.00	Em11/Me19	27	14	51.85
ZM43	6	6	100.00	Em12/Me4	15	4	26.67
Sa72	7	5	71.43	Em12/Me7	23	8	34.78
Sa108	12	12	100.00	Em12/Me19	21	8	38.10
Sa182	6	1	16.67	Em12/Me21	21	8	38.10
ZHY23	8	4	50.00
ZHY24	9	8	88.89
总计Total	265	221	—	总计 Total	601	268	—
平均Average	9.46	7.89	83.40	平均Average	23.12	10.31	44.59

类型 Type		资源数量 Resource number	遗传相似系数 Genetic similarity			遗传距离 Genetic distance
类型 Type		资源数量 Resource number	最小值 Min	最大值 Max	平均 Average	最小值 Min	最大值 Max	平均 Average
总计Total		132	0.598 2	0.960 7	0.813 5	0.048 8	0.538 0	0.230 9
地理区域	赣北Northern of Jiangxi	17	0.788 7	0.958 4	0.867 1	0.051 1	0.273 4	0.162 8
Geographic	赣东Eastern of Jiangxi	11	0.735 6	0.946 9	0.858 4	0.063 0	0.350 1	0.175 9
area	赣中北Central and northern of Jiangxi	8	0.767 9	0.909 9	0.848 3	0.100 1	0.301 0	0.184 0
	赣西Western of Jiangxi	47	0.616 6	0.923 8	0.796 3	0.089 8	0.524 5	0.259 7
	赣中南Central and southern of Jiangxi	37	0.620 1	0.930 7	0.805 8	0.076 4	0.500 2	0.233 3
	赣南Southern of Jiangxi	12	0.662 8	0.908 8	0.788 9	0.103 2	0.447 1	0.252 8
粒色	白色White	46	0.616 6	0.960 7	0.812 5	0.048 8	0.504 1	0.230 9
Seed coat	黑色Black	74	0.632 8	0.958 4	0.839 4	0.051 1	0.513 1	0.197 1
colors	其他粒色Other seed colors	12	0.662 8	0.898 4	0.786 7	0.118 1	0.425 5	0.259 0

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