基于枝条和叶片表型性状的掌叶覆盆子种质资源多样性研究

doi:10.3969/j.issn.1004-1524.2021.09.10

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (9): 1660-1667.DOI: 10.3969/j.issn.1004-1524.2021.09.10

基于枝条和叶片表型性状的掌叶覆盆子种质资源多样性研究

何庆海¹(), 刘本同¹, 周政德², 方茹¹, 杨少宗¹^,^*()

1.浙江省林业科学研究院,浙江杭州 310023
2.武义县三和林木种植场,浙江武义 321202

收稿日期:2020-08-17 出版日期:2021-09-25 发布日期:2021-10-09
通讯作者: 杨少宗
作者简介:* 杨少宗,E-mail: yangsz863@163.com
何庆海(1994—),男,安徽肥东人,硕士,主要从事选择育种与栽培工作。E-mail: 1464333629@qq.com
基金资助:
中央财政林业科技推广示范基金(〔2019〕TS 01号)

Diversity of Rubus chingii germplasm resources based on twig and leaf phenotypic traits

HE Qinghai¹(), LIU Bentong¹, ZHOU Zhengde², FANG Ru¹, YANG Shaozong¹^,^*()

1. Zhejiang Academy of Forestry, Hangzhou 310023, China
2. Wuyi Sanhe Tree Plantation, Wuyi 321202, China

Received:2020-08-17 Online:2021-09-25 Published:2021-10-09
Contact: YANG Shaozong

摘要/Abstract

摘要：

为明确掌叶覆盆子(Rubus chingii H.H. Hu)不同优选种质间枝条和叶片表型性状的变异程度和变异规律,采用方差分析、主成分分析和聚类分析等方法,对20个优选种质枝条和叶片的10个表型性状的变异进行分析,并对聚类分组表型进行评价。研究结果表明,掌叶覆盆子枝条和叶片表型性状变异丰富,变异系数幅度为10.22%~46.63%,平均变异系数为22.02%;不同种质间变异差别明显,RC17平均变异系数最大(18.79%),RC01平均变异系数最小(11.27%)。主成分分析表明,前3个主成分累计贡献率为79.04%,第1主成分贡献率为42.61%;聚类分析结果将20份掌叶覆盆子种质分为3大类,其中第Ⅰ类包括10份,第Ⅱ类包括9份,第Ⅲ类包括1份。Ⅰ类与Ⅱ类组间叶脉角和叶裂数存在显著差异(P<0.05);Ⅰ类与Ⅲ类组间地径、叶片长、叶片宽、叶柄长、叶裂宽和叶脉角存在显著差异(P<0.05);Ⅱ类与Ⅲ类组间地径、叶片长、叶片宽、叶柄长、叶裂宽和叶裂数存在显著差异(P<0.05)。探讨优选种质枝条和叶片表型性状多样性,将为掌叶覆盆子资源保存及构建核心种质提供数据基础以及新品种选育和高效利用提供参考。

关键词: 掌叶覆盆子, 表型性状, 种质资源, 聚类分析

Abstract:

To determine the variation degree and law of twig and leaf phenotypic traits in different elite germplasm of Rubus chingii, the phenotypic variations of 10 twig and leaf phenotypic traits of 20 selected accessions were analyzed by variance analysis, principal component analysis and cluster analysis, and the cluster grouping phenotypes were evaluated. The results showed that the phenotypic traits of Rubus chingii varied widely, the coefficient of variation ranged from 10.22% to 46.63%, and the average coefficient of variation was 22.02%. The average coefficient of variation of RC17 and RC01 were 18.79% and 11.27% respectively. Principal component analysis showed that the cumulative contribution rate of the first three principal components was 79.04%, and that of the first principal component was 42.61%.The 20 germplasms of Rubus chingii were divided into 3 groups, including 10 in clusterⅠ, 9 in cluster Ⅱ and 1 in cluster Ⅲ. There were significant (P<0.05) differences in leaf vein angle and lobed number between cluster Ⅰ and cluster Ⅱ. The differences of ground diameter, leaf length, leaf width, petiole length, leaf fissure width and leaf vein angle between cluster Ⅰ and cluster Ⅲ were also significant (P<0.05). The significant (P<0.05) difference was also observed in ground diameter, leaf length, leaf width, petiole length, width of leaf lobes and number of leaf lobes between cluster Ⅱ and cluster Ⅲ. It was concluded that this study would not only provide data basis for resource conservation and core collection construction, but also provide reference for description and efficient utilization of new variety breeding.

Key words: Rubus chingii H.H.Hu, phenotypic traits, germplasm resources, cluster analysis

中图分类号:

S668.9

何庆海, 刘本同, 周政德, 方茹, 杨少宗. 基于枝条和叶片表型性状的掌叶覆盆子种质资源多样性研究[J]. 浙江农业学报, 2021, 33(9): 1660-1667.

HE Qinghai, LIU Bentong, ZHOU Zhengde, FANG Ru, YANG Shaozong. Diversity of Rubus chingii germplasm resources based on twig and leaf phenotypic traits[J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1660-1667.

图/表 6

参考文献 24

[1]	程丹, 李洁, 周斌, 等. 覆盆子化学成分与药理作用研究进展[J]. 中药材, 2012, 35(11):1873-1876.
	CHENG D, LI J, ZHOU B, et al. Studies on chemical constituents and pharmacological action in fruits of Rubus chingii[J]. Journal of Chinese Medicinal Materials, 2012, 35(11):1873-1876.(in Chinese)
[2]	郭启雷, 杨峻山. 掌叶覆盆子的化学成分研究[J]. 中国中药杂志, 2005, 30(3):1141-1143.
	GUO Q L, YANG J S. Studies on chemical constituents in fruit of Rubus chingii[J]. China Journal of Chinese Materia Medica, 2005, 30(3):1141-1143.(in Chinese with English abstract)
[3]	肖洪明, 祖灵博, 李石平, 等. 掌叶覆盆子化学成分的研究[J]. 中国药物化学杂志, 2011, 21(3):220-226.
	XIAO H M, ZU L B, LI S P, et al. Chemical constituents from dried fruits of Rubus chingii[J]. Chinese Journal of Medicinal Chemistry, 2011, 21(3):220-226.(in Chinese with English abstract)
[4]	傅承新, 沈朝栋, 黄爱军. 浙江悬钩子属植物的综合研究:资源调查、引种及开发利用前景[J]. 浙江农业大学学报, 1995, 21(4):393-397.
	FU C X, SHEN C D, HUANG A J. A comprekensive study on Rubus L. in Zhejiang Province: wild specific resources, transplantation and exploltation[J]. Journal of Zhejiang Agricultural University, 1995, 21(4):393-397. (in Chinese)
[5]	潘彬荣, 许立奎, 阮柏苗, 等. 掌叶覆盆子优株的复选及繁殖示范推广研究[J]. 安徽农业科学, 2010, 38(34):19302-19304.
	PAN B R, XU L K, RUAN B M, et al. Study on repeated selection of Rubus chingii Hu superior individual plants and their demonstration and extension[J]. Journal of Anhui Agricultural Sciences, 2010, 38(34):19302-19304.(in Chinese with English abstract)
[6]	汪黑铁. 掌叶覆盆子繁育栽培技术[J]. 现代农业科技, 2019(20):73-74.
	WANG H T. The breeding and cultivation techniques of Rubus chingii[J]. Modern Agricultural Science and Technology, 2019(20):73-74. (in Chinese)
[7]	邬枭楠. 掌叶覆盆子山地种植技术[J]. 现代农业科技, 2016(15):170.
	WU X N. Planting techniques of Rubus chingii in mountainous area[J]. Modern Agricultural Science and Technology, 2016(15):170.(in Chinese)
[8]	游晓庆, 陈慧, 李晓辉, 等. 不同种源掌叶覆盆子种子和果实表型性状及发芽率研究[J]. 南方林业科学, 2019, 47(3):16-19.
	YOU X Q, CHEN H, LI X H, et al. Phenotypic traits and germination rate of Rubus chingii seeds and fruits from different sources[J]. South China Forestry Science, 2019, 47(3):16-19.(in Chinese with English abstract)
[9]	潘彬荣, 许立奎, 阮柏苗, 等. 掌叶覆盆子优株的生长习性调查及选育[J]. 温州农业科技, 2011(1):18-20.
	PAN B R, XU L K, RUAN B M, et al. The growth habit investigation and breeding of Rubus chingii Hu superior individual plants[J]. Wenzhou Agricultural Science and Technology, 2011(1):18-20.(in Chinese)
[10]	张莹, 曹玉芬, 霍宏亮, 等. 基于花表型性状的梨种质资源多样性研究[J]. 园艺学报, 2016, 43(7):1245-1256.
	ZHANG Y, CAO Y F, HUO H L, et al. Research on diversity of pear germplasm resources based on flowers phenotype traits[J]. Acta Horticulturae Sinica, 2016, 43(7):1245-1256.(in Chinese with English abstract)
[11]	王春芳, 余兴华, 王先宏, 等. 基于主要表型性状的不同采集地斑茅种质资源遗传多样性分析[J]. 热带作物学报, 2020, 41(6):1108-1116.
	WANG C F, YU X H, WANG X H, et al. Genetic diversity analysis based on main phenotypic characteristics of Erianthus arundinaceum germplasm from different regions[J]. Chinese Journal of Tropical Crops, 2020, 41(6):1108-1116.(in Chinese with English abstract)
[12]	赵孟良, 韩睿, 田闵玉, 等. 126份芜菁种质资源地上部表型的多样性分析[J]. 分子植物育种, 2021, 19(1):318-332.
	ZHAO M L, HAN R, TIAN M Y, et al. Diversity analysis of aboveground traits of 126 trunip (Brassica rapa var. rapa) germplasm resources[J]. Molecular Plant Breeding, 2021, 19(1):318-332. (in Chinese with English abstract)
[13]	白光红, 张义荣, 刘弋菊, 等. ImageJ图象处理软件在测量玉米子粒大小中的应用[J]. 玉米科学, 2009, 17(1):147-151.
	BAI G H, ZHANG Y R, LIU Y J, et al. Application of ImageJ analysis software in measuring kernel size of maize seeds[J]. Journal of Maize Sciences, 2009, 17(1):147-151.(in Chinese with English abstract)
[14]	宋军生, 党占海, 张建平, 等. 油用亚麻品种资源农艺性状的主成分及聚类分析[J]. 西南农业学报, 2015, 28(2):492-497.
	SONG J S, DANG Z H, ZHANG J P, et al. Principal component analysis and cluster analysis of oil flax germplasm based on agronomic traits[J]. Southwest China Journal of Agricultural Sciences, 2015, 28(2):492-497.(in Chinese with English abstract)
[15]	崔翠, 周清元, 王利鹃, 等. 亚麻种质主要农艺性状主成分分析与综合评价[J]. 西南大学学报(自然科学版), 2016, 38(12):10-18.
	CUI C, ZHOU Q Y, WANG L J, et al. The cluster analysis and comprehensive evaluation of flax germplasm based on principal components of agronomic traits[J]. Journal of Southwest University (Natural Science Edition), 2016, 38(12):10-18.(in Chinese with English abstract)
[16]	张武君, 陈菁瑛, 刘保财, 等. 37份福建山药地方品种主要性状遗传变异研究[J]. 福建农业学报, 2019, 34(11):1246-1254.
	ZHANG W J, CHEN J Y, LIU B C, et al. Genetic variations on major traits of 37 Chinese yam germplasms[J]. Fujian Journal of Agricultural Sciences, 2019, 34(11):1246-1254.(in Chinese with English abstract)
[17]	CUI H L, CHEN C R, HUANG N Z, et al. Association analysis of yield, oil and fatty acid content, and main phenotypic traits in Paeonia rockii as an oil crop[J]. The Journal of Horticultural Science and Biotechnology, 2018, 93(4):425-432. DOI URL
[18]	何庆海, 杨少宗, 李因刚, 等. 枫香树种群种子与果实表型性状变异分析[J]. 植物生态学报, 2018, 42(7):752-763. DOI
	HE Q H, YANG S Z, LI Y G, et al. Phenotypic variations in seed and fruit traits of Liquidambar formosana populations[J]. Chinese Journal of Plant Ecology, 2018, 42(7):752-763.(in Chinese with English abstract) DOI URL
[19]	孙荣喜, 郑勇奇, 张川红, 等. 不同群体国槐种子表型变异研究[J]. 河北农业大学学报, 2011, 34(3):65-70.
	SUN R X, ZHENG Y Q, ZHANG C H, et al. Study on the seed phenotypic variation of Sophora japonica L. in different populations[J]. Journal of Agricultural University of Hebei, 2011, 34(3):65-70.(in Chinese with English abstract)
[20]	刁松锋, 邵文豪, 姜景民, 等. 基于种实性状的无患子天然群体表型多样性研究[J]. 生态学报, 2014, 34(6):1451-1460.
	DIAO S F, SHAO W H, JIANG J M, et al. Phenotypic diversity in natural populationsof Sapindus mukorossi based on fruit and seed traits[J]. Acta Ecologica Sinica, 2014, 34(6):1451-1460.(in Chinese with English abstract)
[21]	张燕, 杨衍, 田丽波, 等. 基于表型性状的苦瓜种质资源评价和遗传多样性的分析[J]. 分子植物育种, 2016, 14(1):239-250.
	ZHANG Y, YANG Y, TIAN L B, et al. Evaluation and genetic diversity of bitter gourd varieties based on phenotypic traits[J]. Molecular Plant Breeding, 2016, 14(1):239-250.(in Chinese with English abstract)
[22]	张怀山, 夏曾润, 代立兰, 等. 中型狼尾草种质资源表型性状的多样性[J]. 西北农业学报, 2014, 23(12):51-59.
	ZHANG H S, XIA Z R, DAI L L, et al. Phenotypic diversity of Pennisetum longissimum var.intermedium germplasm resources[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2014, 23(12):51-59.(in Chinese with English abstract)
[23]	王海平, 李锡香, 沈镝, 等. 基于表型性状的中国大蒜资源遗传多样性分析[J]. 植物遗传资源学报, 2014, 15(1):24-31.
	WANG H P, LI X X, SHEN D, et al. Evaluation on genetic diversity of garlic(Allium sativum L.) clones in China based on morphological characters[J]. Journal of Plant Genetic Resources, 2014, 15(1):24-31.(in Chinese with English abstract)
[24]	芮文婧, 王晓敏, 张倩男, 等. 番茄353份种质资源表型性状遗传多样性分析[J]. 园艺学报, 2018, 45(3):561-570.
	RUI W J, WANG X M, ZHANG Q N, et al. Genetic diversity analysis of 353 tomato germplasm resources by phenotypic traits[J]. Acta Horticulturae Sinica, 2018, 45(3):561-570.(in Chinese with English abstract)

性状 Characters	平均值 Average	标准差 Standard deviation	极差 Range	最小值 Minimum	最大值 Maximum	变异系数 Coefficient of variation/%
株高Plant height/m	1.83	0.28	1.25	1.25	2.50	15.48
地径 Ground diameter/mm	16.47	2.85	14.30	8.70	23.00	17.32
冠幅 Crown breadth/m	1.37	0.35	1.60	0.60	2.20	25.59
分枝数 Branching number	3.06	1.43	6.00	1.00	7.00	46.63
叶片长Leaf length/cm	11.09	1.79	9.32	7.35	16.67	16.17
叶片宽 Leaf width/cm	9.76	2.06	11.30	5.54	16.84	21.15
叶柄长Petiole length/cm	3.74	0.73	4.10	1.95	6.05	19.45
叶裂宽Width of leaf lobes/cm	1.01	0.36	1.97	0.46	2.43	35.36
叶脉角Leaf vein angle/(°)	51.37	5.25	21.89	40.67	62.56	10.22
叶裂数 Number of leaf lobes	5.38	0.69	2.00	4.00	6.00	12.83

性状 Characters	平均值 Average	标准差 Standard deviation	极差 Range	最小值 Minimum	最大值 Maximum	变异系数 Coefficient of variation/%
株高Plant height/m	1.83	0.28	1.25	1.25	2.50	15.48
地径 Ground diameter/mm	16.47	2.85	14.30	8.70	23.00	17.32
冠幅 Crown breadth/m	1.37	0.35	1.60	0.60	2.20	25.59
分枝数 Branching number	3.06	1.43	6.00	1.00	7.00	46.63
叶片长Leaf length/cm	11.09	1.79	9.32	7.35	16.67	16.17
叶片宽 Leaf width/cm	9.76	2.06	11.30	5.54	16.84	21.15
叶柄长Petiole length/cm	3.74	0.73	4.10	1.95	6.05	19.45
叶裂宽Width of leaf lobes/cm	1.01	0.36	1.97	0.46	2.43	35.36
叶脉角Leaf vein angle/(°)	51.37	5.25	21.89	40.67	62.56	10.22
叶裂数 Number of leaf lobes	5.38	0.69	2.00	4.00	6.00	12.83

编号 No.	株高 Plant height	地径 Ground diameter	冠幅 Crown breadth	分枝数 Branching number	叶片长 Leaf length	叶片宽 Leaf width	叶柄长 Petiole length	叶裂宽 Width of leaf lobes	叶脉角 Leaf vein angle	叶裂数 Number of leaf lobes
RC01	8.93	7.98	24.48	34.40	5.81	8.55	9.85	7.48	5.20	0
RC02	6.77	15.57	11.15	24.85	6.50	9.27	10.77	17.03	7.63	8.18
RC03	4.34	16.02	19.23	25.91	14.13	16.77	13.96	46.93	6.24	5.31
RC04	16.12	18.82	21.13	31.62	6.63	7.00	12.06	17.05	7.09	10.90
RC05	11.37	10.56	32.32	38.03	11.06	17.24	16.39	16.71	8.51	12.99
RC06	8.74	4.18	18.12	81.31	5.13	4.40	17.41	33.05	7.92	2.26
RC07	14.44	18.38	17.56	39.49	16.91	12.08	24.76	21.82	12.57	9.91
RC08	14.52	10.68	19.93	38.03	7.71	16.76	9.98	38.08	11.88	9.30
RC09	15.06	8.86	19.93	39.53	15.75	20.34	25.05	14.94	8.77	19.37
RC10	6.76	4.96	23.96	39.12	7.09	14.38	8.19	14.08	7.15	0.00
RC11	11.90	16.13	34.05	33.33	12.93	12.94	16.15	15.14	9.66	18.69
RC12	3.89	8.90	25.49	23.57	12.51	12.17	19.21	24.07	6.57	12.57
RC13	7.28	12.66	16.13	47.51	3.11	7.17	1.46	16.57	8.46	5.08
RC14	7.89	22.76	21.63	34.23	13.38	15.76	21.63	33.85	4.77	9.91
RC15	5.54	29.06	24.09	26.31	10.64	23.30	13.14	13.11	8.43	10.65
RC16	7.47	5.28	13.56	30.62	15.98	14.29	16.50	11.58	8.31	8.44
RC17	7.60	9.74	20.22	22.82	14.79	12.59	17.90	18.82	7.76	8.60
RC18	9.10	6.46	19.93	60.11	5.04	4.30	6.20	21.13	7.62	5.36
RC19	4.87	8.23	18.92	39.12	16.55	15.41	20.47	19.59	5.23	3.99
RC20	9.41	14.29	17.12	23.57	6.31	9.09	15.92	21.35	15.92	8.44
平均值Average	9.10	12.48	20.95	36.67	10.40	12.69	14.85	21.12	8.29	8.50

编号 No.	株高 Plant height	地径 Ground diameter	冠幅 Crown breadth	分枝数 Branching number	叶片长 Leaf length	叶片宽 Leaf width	叶柄长 Petiole length	叶裂宽 Width of leaf lobes	叶脉角 Leaf vein angle	叶裂数 Number of leaf lobes
RC01	8.93	7.98	24.48	34.40	5.81	8.55	9.85	7.48	5.20	0
RC02	6.77	15.57	11.15	24.85	6.50	9.27	10.77	17.03	7.63	8.18
RC03	4.34	16.02	19.23	25.91	14.13	16.77	13.96	46.93	6.24	5.31
RC04	16.12	18.82	21.13	31.62	6.63	7.00	12.06	17.05	7.09	10.90
RC05	11.37	10.56	32.32	38.03	11.06	17.24	16.39	16.71	8.51	12.99
RC06	8.74	4.18	18.12	81.31	5.13	4.40	17.41	33.05	7.92	2.26
RC07	14.44	18.38	17.56	39.49	16.91	12.08	24.76	21.82	12.57	9.91
RC08	14.52	10.68	19.93	38.03	7.71	16.76	9.98	38.08	11.88	9.30
RC09	15.06	8.86	19.93	39.53	15.75	20.34	25.05	14.94	8.77	19.37
RC10	6.76	4.96	23.96	39.12	7.09	14.38	8.19	14.08	7.15	0.00
RC11	11.90	16.13	34.05	33.33	12.93	12.94	16.15	15.14	9.66	18.69
RC12	3.89	8.90	25.49	23.57	12.51	12.17	19.21	24.07	6.57	12.57
RC13	7.28	12.66	16.13	47.51	3.11	7.17	1.46	16.57	8.46	5.08
RC14	7.89	22.76	21.63	34.23	13.38	15.76	21.63	33.85	4.77	9.91
RC15	5.54	29.06	24.09	26.31	10.64	23.30	13.14	13.11	8.43	10.65
RC16	7.47	5.28	13.56	30.62	15.98	14.29	16.50	11.58	8.31	8.44
RC17	7.60	9.74	20.22	22.82	14.79	12.59	17.90	18.82	7.76	8.60
RC18	9.10	6.46	19.93	60.11	5.04	4.30	6.20	21.13	7.62	5.36
RC19	4.87	8.23	18.92	39.12	16.55	15.41	20.47	19.59	5.23	3.99
RC20	9.41	14.29	17.12	23.57	6.31	9.09	15.92	21.35	15.92	8.44
平均值Average	9.10	12.48	20.95	36.67	10.40	12.69	14.85	21.12	8.29	8.50

性状 Characters	主成分载荷矩阵Principal component load matrix			主成分特征向量Principal component eigenvector
性状 Characters	1	2	3	1	2	3
株高Plant hight	0.24	0.59	0.56	0.06	0.29	0.35
地径 Ground diameter	0.71	0.31	-0.06	0.17	0.15	-0.04
冠幅 Crown breadth	0.38	0.64	-0.50	0.09	0.31	-0.31
分枝数 Branching number	0.24	0.77	-0.31	0.06	0.37	-0.20
叶片长 length of leaf	0.96	-0.04	0.14	0.23	-0.02	0.09
叶片宽 Width of leaf	0.95	-0.21	0.12	0.22	-0.10	0.08
叶柄长Length of petiole	0.83	0.15	0.05	0.20	0.07	0.03
叶裂宽 Lplit width of leaf	0.68	-0.48	0.38	0.16	-0.23	0.24
叶脉角 Vein angle	-0.14	0.38	0.78	-0.03	0.19	0.49
叶裂数 Split number of leaf	0.70	-0.42	-0.37	0.17	-0.20	-0.23

基于枝条和叶片表型性状的掌叶覆盆子种质资源多样性研究

Diversity of Rubus chingii germplasm resources based on twig and leaf phenotypic traits

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成分 Components	初始特征值Initial eigenvalue			主成分特征值Principal component eigenvalu
成分 Components	特征值 Eigenvalue	方差贡献率 Variance contribution rate/%	累积方差贡献率 Cumulative variance contribution rate/%	特征值 Eigenvalue	方差贡献率 Variance contribution rate/%	累积方差贡献率 Cumulative variance contribution rate/%
1	4.26	42.61	42.61	4.26	42.61	42.61
2	2.05	20.52	63.13	2.05	20.52	63.13
3	1.59	15.91	79.04	1.59	15.91	79.04
4	0.87	8.74	87.78
5	0.44	4.43	92.21

性状Characters	第Ⅰ类ClusterⅠ	第Ⅱ类ClusterⅡ	第Ⅲ类Cluster Ⅲ
株高Plant height/m	1.76±0.27 a	1.91±0.30 a	1.86±0.17 a
地径 Ground diameter/mm	17.00±2.55 b	15.65 ±3.01 b	19.20 ±1.53 a
冠幅 Crown breadth/m	1.43 ±0.34 a	1.30±0.36 a	1.30±0.32 a
分枝数 Branching number	3.02±1.56 a	3.13 ±1.32 a	2.60±0.89 a
叶片长Length of leaf/cm	11.10±1.53 b	10.67±1.55 b	15.41±0.90 a
叶片宽 Width of leaf/cm	9.88±1.68 b	9.13±1.74 b	15.33±1.31 a
叶柄长Length of petiole/cm	3.72±0.72 b	3.63±0.63 b	4.99±0.49 a
叶裂宽 Split width of leaf/cm	1.00±0.29 b	0.95±0.34 b	1.91±0.14 a
叶脉角 Vein angle/(°)	48.31±4.64 b	54.51±3.99 a	53.19±2.77 a
叶裂数 Split number of leaf	5.61±0.59 a	5.08±0.70 b	6.00±0 a

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