浙江农业学报 ›› 2021, Vol. 33 ›› Issue (9): 1770-1778.DOI: 10.3969/j.issn.1004-1524.2021.09.21
孙丽萍1,2(), 白琳琳2,3, 干雅婷1, 陈雪雲2,3, 王柳2, 张宜明1,*(
), 何开雨2,*(
), 徐霞红2
收稿日期:
2020-08-10
出版日期:
2021-09-25
发布日期:
2021-10-09
通讯作者:
张宜明,何开雨
作者简介:
何开雨,E-mail: hekaiyu@zaas.ac.cn基金资助:
SUN Liping1,2(), BAI Linlin2,3, GAN Yating1, CHEN Xueyun2,3, WANG Liu2, ZHANG Yiming1,*(
), HE Kaiyu2,*(
), XU Xiahong2
Received:
2020-08-10
Online:
2021-09-25
Published:
2021-10-09
Contact:
ZHANG Yiming,HE Kaiyu
摘要:
DNA的结构具有多样性,除经典的双螺旋结构外,近年来的研究发现和组装获得了更为多样的DNA结构类型,如三螺旋结构、发夹结构、i-motif结构、G-四链体等非传统结构。这些特殊结构具有一定的功能,例如,G-四链体可与氯高铁血红素结合形成具有辣根过氧化物酶活性的复合物,也可增强某些染料分子的荧光强度。此外,G-四链体的形成与稳定性可控、可设计,是构建化学生物传感的良好材料。以一些典型的研究工作为例,本文对近年来G-四链体在农产品中农兽药残留及重金属离子快速检测中的应用进行了总结,并对其应用前景进行展望。
中图分类号:
孙丽萍, 白琳琳, 干雅婷, 陈雪雲, 王柳, 张宜明, 何开雨, 徐霞红. 基于DNA G-四链体的农兽药残留与重金属离子快速检测研究进展[J]. 浙江农业学报, 2021, 33(9): 1770-1778.
SUN Liping, BAI Linlin, GAN Yating, CHEN Xueyun, WANG Liu, ZHANG Yiming, HE Kaiyu, XU Xiahong. Research progress of rapid detection of pesticides and veterinary drugs residues and heavy metal ions based on DNA G-quadruplex[J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1770-1778.
图1 G-四分体及G-四链体 A,G-四分体结构;B,分子内折叠形成G-四链体(反平行结构);C,双分子折叠形成G-四链体(反平行结构);D,四分子折叠形成G-四链体(平行结构)。
Fig.1 G-tetrad and G-quadruplexes A, G-tetrad structure; B, Monomolecular folded G-quadruplex (Antiparallel structure); C, Bimolecular folded G-quadruplex (Antiparallel structure); D, Tetramolecular folded G-quadruplex (Parallel structure).
图2 染料分子结构式 A,卟啉[33];B,硫磺素T[34];C,噻唑橙[35];D,N-甲基吗啡啉[36]。
Fig.2 Molecular structural formula of dyes A, Protoporphyrin IX[33]; B, Thioflavin T[34]; C, Thiazole Orange[35]; D, N-methylmorpholine[36].
图3 基于G-四链体传感快速检测农产品中兽药残留 A,基于DNA G-四链体和三螺旋结构快速检测四环素[39];B,利用DNA G-四链体构建SERS传感平台快速检测孔雀石绿[40]。
Fig.3 Rapid detection of veterinary drug residues in agricultural products based on G-quadruplex sensors A, Scheme of tetracycline rapid detection based on DNA G-quadruplex and triple helix[39]; B, Scheme of G-quadruplex based SERS sensing platform for the rapid detection of malachite green[40].
图4 基于G-四链体传感快速检测农产品中农药残留 A,利用乙醇胺适配体形成的G-四链体快速检测乙醇胺原理图[42];B,基于DNA 适配体和G-四链体免标记无酶荧光生物传感快速检测水胺硫磷[43];C,基于DNA三螺旋结构分子开关和G-四链体荧光生物传感快速检测啶虫脒[44]。
Fig.4 Rapid detection of pesticide residues in agricultural products based on G-quadruplex sensors A, The rapid detection of ethanolamine based on G-quadruplex formed by ethanolamine aptamer[42]; B, The label-free and enzyme-free fluorescent biosensor for the rapid detection of ICP[43]; C, Fluorescence biosensor for the detection of acetamiprid based on DNA triple helix molecular switch and G-quadruplex[44].
图5 基于G-四链体传感快速检测农产品中重金属离子 A,基于DNA G-四链体的Hg2+免标记传感器[45];B,G-四链体-hemin催化放大电化学信号灵敏检测Pb2+[46];C,基于G-四链体的Ag+快速检测[47]。
Fig.5 Rapid detection of heavy metal ions in agricultural products based on G-quadruplex sensors A, Scheme of the label-free Hg2+ sensor based on G-quadruplex[45]; B, DNA G-quadruplex-hemin amplified signal for the sensitive electrochemical detection of Pb2+[46]; C, DNA G-quadruplex based Ag+ biosensor[47].
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