浙江农业学报 ›› 2022, Vol. 34 ›› Issue (2): 221-231.DOI: 10.3969/j.issn.1004-1524.2022.02.02
收稿日期:
2021-05-19
出版日期:
2022-02-25
发布日期:
2022-03-02
通讯作者:
丁波
作者简介:
丁波,E-mail: lifescience2021@163.com基金资助:
JIA Liqiang1(), ZHAO Qiufang2, CHEN Shu2, DING Bo1,*()
Received:
2021-05-19
Online:
2022-02-25
Published:
2022-03-02
Contact:
DING Bo
摘要:
为探究不同逆境胁迫(盐、干旱、温度和硝态氮/铵态氮缺乏胁迫)对玉米ZmbZIP基因表达模式的影响,以玉米骨干自交系郑58为实验材料,设置200 mmol·L-1 NaCl、20% PEG6000、4 ℃低温、硝态氮或铵态氮缺乏胁迫试验,探测其对bZIP家族基因G亚家族成员表达模式的影响。进化树分析结果显示,G亚家族成员进一步细分为3个亚组。qRT-PCR分析结果表明,15个ZmbZIPs基因具有不同的组织表达模式,表明这些基因在玉米生长发育中发挥不同的作用。人为模拟盐、干旱、低温和氮缺乏胁迫实验中,20个ZmbZIPs基因受不同逆境因子的广泛调控,预示ZmbZIP广泛参与调控玉米应答逆境胁迫响应途径。
中图分类号:
贾利强, 赵秋芳, 陈曙, 丁波. 玉米转录因子bZIP G亚家族基因的表达模式[J]. 浙江农业学报, 2022, 34(2): 221-231.
JIA Liqiang, ZHAO Qiufang, CHEN Shu, DING Bo. Expression analysis of bZIP G subfamily genes in maize[J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 221-231.
基因名称 Gene name | 基因号 Gene locus | 上游引物 Forward primers (5'→3') | 下游引物 Reverse primers (5'→3') |
---|---|---|---|
ZmbZIP2 | Zm00001d028372 | CATCCATCTGTAGCTCCAAGCCATC | CTGTCCCGTTTGGTAACTGCATAGG |
ZmbZIP20 | Zm00001d034571 | GGCACGCTCAGATCAGAACTCGAC | ATGCTCAGAGTCGTCACAACGCTAG |
ZmbZIP26 | Zm00001d004897 | CAGCAACGGGAAAGGAAGCCAC | GGCTGTGAAAACGCACTGGTTG |
ZmbZIP39 | Zm00001d005244 | GGGAATGCTACCATTTTGCCACAAC | TTTCGCGCTTCAATTCCCTCTCATC |
ZmbZIP40 | Zm00001d040500 | GCCAACACAGCAAGCTCTGCTC | CACGTTCATCCTGTATCCATTGCTC |
ZmbZIP50 | Zm00001d041142 | CCCAGTGCTACAAATGTCGCGAAC | ACGAGCAGGCAGGAACAGCAAC |
ZmbZIP51 | Zm00001d043908 | CCCTGGAATAGCGCCTGGTCATG | CTCCGCACTCAATGGGTTTACCAC |
ZmbZIP67 | Zm00001d043992 | TCATCACGACCAGAGCCAAATCCA | ACCGCAATTGTTCCCGTGACGTTG |
ZmbZIP73 | Zm00001d052562 | CCTATGCCAACAAATGGACATGCTG | CACTCTCACTCTCACTTCCACTCTC |
ZmbZIP96 | Zm00001d053967 | CAATGAGCGTGGAGCCAGCCAA | TACATCGCTTGAGCCTTCTGCAC |
ZmbZIP97 | Zm00001d015118 | CGTCTGCTCCATCCGCCAAC | AGAATCGCCATCTTCCTTCCCATC |
ZmbZIP100 | Zm00001d039065 | GCATCGAGCCGCCGAAGTTG | TGCCTCCAGCACAGTTCCCATTG |
ZmbZIP123 | Zm00001d039206 | CTCGTCCTGTCCGAGCACCTC | GTGAGTCCAACTACTGGCGGCTTAC |
ZmbZIP133 | Zm00001d019166 | GGAAAGGAAGAAGGCGTACCTGAG | GCCTCTTCTGTTGTTGACAGTGGTG |
ZmbZIP125 | Zm00001d045288 | GAACGGCCACACTTACCAGG | GATACGATCACGACGGCTCTAAACC |
ZmbZIP136 | Zm00001d047967 | ATGGCGACGTTGGTGTGCCAC | GAGCGAGCCGTGATGGTGGTG |
ZmbZIP138 | Zm00001d046664 | CAGCGTGGACCAGTTCTTCGAC | CATCGCCATCCACCTCCACCG |
ZmbZIP140 | Zm00001d024041 | AGAACAACAACCGCAGACTCTCAAC | CCGCCTCCCACCTTCAACGAC |
ZmbZIP142 | Zm00001d024285 | GCAACGCCTATACCGTTCTACCAG | CGGTGGATAATGGCAGGTCTGAG |
表1 ZmbZIP基因的实时荧光定量引物
Table 1 qRT-PCR primers of ZmbZIP gene
基因名称 Gene name | 基因号 Gene locus | 上游引物 Forward primers (5'→3') | 下游引物 Reverse primers (5'→3') |
---|---|---|---|
ZmbZIP2 | Zm00001d028372 | CATCCATCTGTAGCTCCAAGCCATC | CTGTCCCGTTTGGTAACTGCATAGG |
ZmbZIP20 | Zm00001d034571 | GGCACGCTCAGATCAGAACTCGAC | ATGCTCAGAGTCGTCACAACGCTAG |
ZmbZIP26 | Zm00001d004897 | CAGCAACGGGAAAGGAAGCCAC | GGCTGTGAAAACGCACTGGTTG |
ZmbZIP39 | Zm00001d005244 | GGGAATGCTACCATTTTGCCACAAC | TTTCGCGCTTCAATTCCCTCTCATC |
ZmbZIP40 | Zm00001d040500 | GCCAACACAGCAAGCTCTGCTC | CACGTTCATCCTGTATCCATTGCTC |
ZmbZIP50 | Zm00001d041142 | CCCAGTGCTACAAATGTCGCGAAC | ACGAGCAGGCAGGAACAGCAAC |
ZmbZIP51 | Zm00001d043908 | CCCTGGAATAGCGCCTGGTCATG | CTCCGCACTCAATGGGTTTACCAC |
ZmbZIP67 | Zm00001d043992 | TCATCACGACCAGAGCCAAATCCA | ACCGCAATTGTTCCCGTGACGTTG |
ZmbZIP73 | Zm00001d052562 | CCTATGCCAACAAATGGACATGCTG | CACTCTCACTCTCACTTCCACTCTC |
ZmbZIP96 | Zm00001d053967 | CAATGAGCGTGGAGCCAGCCAA | TACATCGCTTGAGCCTTCTGCAC |
ZmbZIP97 | Zm00001d015118 | CGTCTGCTCCATCCGCCAAC | AGAATCGCCATCTTCCTTCCCATC |
ZmbZIP100 | Zm00001d039065 | GCATCGAGCCGCCGAAGTTG | TGCCTCCAGCACAGTTCCCATTG |
ZmbZIP123 | Zm00001d039206 | CTCGTCCTGTCCGAGCACCTC | GTGAGTCCAACTACTGGCGGCTTAC |
ZmbZIP133 | Zm00001d019166 | GGAAAGGAAGAAGGCGTACCTGAG | GCCTCTTCTGTTGTTGACAGTGGTG |
ZmbZIP125 | Zm00001d045288 | GAACGGCCACACTTACCAGG | GATACGATCACGACGGCTCTAAACC |
ZmbZIP136 | Zm00001d047967 | ATGGCGACGTTGGTGTGCCAC | GAGCGAGCCGTGATGGTGGTG |
ZmbZIP138 | Zm00001d046664 | CAGCGTGGACCAGTTCTTCGAC | CATCGCCATCCACCTCCACCG |
ZmbZIP140 | Zm00001d024041 | AGAACAACAACCGCAGACTCTCAAC | CCGCCTCCCACCTTCAACGAC |
ZmbZIP142 | Zm00001d024285 | GCAACGCCTATACCGTTCTACCAG | CGGTGGATAATGGCAGGTCTGAG |
基因ID Gene ID | 基因名称 Gene name | 长度 Length/bp | 分子量 Molecular mass/ku | 等电点 Isoelectric point | 染色体 Chromosome | 可变剪切 Splice variants |
---|---|---|---|---|---|---|
Zm00001d028372 | ZmbZIP2 | 583 | 62.46 | 9.55 | Chr1:32433751-32442505 | 17 |
Zm00001d034571 | ZmbZIP20 | 356 | 37.47 | 7.59 | Chr1:296596877-296600683 | 3 |
Zm00001d004897 | ZmbZIP24 | 347 | 36.48 | 8.35 | Chr2:147657998-147659916 | 1 |
Zm00001d005244 | ZmbZIP26 | 371 | 38.75 | 7.16 | Chr2:165521128-165527158 | 9 |
Zm00001d040500 | ZmbZIP39 | 194 | 21.09 | 10.13 | Chr3:47004098-47005326 | 1 |
Zm00001d041142 | ZmbZIP40 | 382 | 40.63 | 8.41 | Chr3:100232730-100240533 | 24 |
Zm00001d043908 | ZmbZIP50 | 191 | 20.72 | 9.34 | Chr3:213533138-213534279 | 1 |
Zm00001d043992 | ZmbZIP51 | 340 | 37.03 | 8.8 | Chr3:215850507-215866477 | 3 |
Zm00001d052562 | ZmbZIP60 | 417 | 43.86 | 7.31 | Chr4:193207888-193210831 | 1 |
Zm00001d053967 | ZmbZIP67 | 314 | 33.62 | 6.17 | Chr4:244414417-244418397 | 1 |
Zm00001d015118 | ZmbZIP73 | 290 | 31.01 | 5.22 | Chr5:76331375-76334353 | 1 |
Zm00001d039065 | ZmbZIP96 | 377 | 40.12 | 7.51 | Chr6:169491185-169496107 | 10 |
Zm00001d039206 | ZmbZIP97 | 208 | 22.36 | 6.84 | Chr6:172509105-172510659 | 1 |
Zm00001d019166 | ZmbZIP100 | 379 | 39.68 | 6.88 | Chr7:19967615-19972466 | 8 |
Zm00001d045288 | ZmbZIP123 | 547 | 60.82 | 7.95 | Chr9:18011858-18027409 | 1 |
Zm00001d047967 | ZmbZIP133 | 423 | 45.11 | 9.94 | Chr9:146611340-146618721 | 4 |
Zm00001d046664 | ZmbZIP136 | 187 | 20.02 | 8.56 | Chr10:5649486-5650525 | 1 |
Zm00001d024041 | ZmbZIP138 | 282 | 29.93 | 7.12 | Chr10:39073197-39078744 | 11 |
Zm00001d024285 | ZmbZIP140 | 213 | 23.6 | 6.03 | Chr10:61712124-61716615 | 1 |
Zm00001d025638 | ZmbZIP142 | 273 | 30.15 | 8.45 | Chr10:124742514-124747717 | 1 |
表2 玉米bZIP家族基因基本信息
Table 2 Basic information of bZIPfamily gene in maize
基因ID Gene ID | 基因名称 Gene name | 长度 Length/bp | 分子量 Molecular mass/ku | 等电点 Isoelectric point | 染色体 Chromosome | 可变剪切 Splice variants |
---|---|---|---|---|---|---|
Zm00001d028372 | ZmbZIP2 | 583 | 62.46 | 9.55 | Chr1:32433751-32442505 | 17 |
Zm00001d034571 | ZmbZIP20 | 356 | 37.47 | 7.59 | Chr1:296596877-296600683 | 3 |
Zm00001d004897 | ZmbZIP24 | 347 | 36.48 | 8.35 | Chr2:147657998-147659916 | 1 |
Zm00001d005244 | ZmbZIP26 | 371 | 38.75 | 7.16 | Chr2:165521128-165527158 | 9 |
Zm00001d040500 | ZmbZIP39 | 194 | 21.09 | 10.13 | Chr3:47004098-47005326 | 1 |
Zm00001d041142 | ZmbZIP40 | 382 | 40.63 | 8.41 | Chr3:100232730-100240533 | 24 |
Zm00001d043908 | ZmbZIP50 | 191 | 20.72 | 9.34 | Chr3:213533138-213534279 | 1 |
Zm00001d043992 | ZmbZIP51 | 340 | 37.03 | 8.8 | Chr3:215850507-215866477 | 3 |
Zm00001d052562 | ZmbZIP60 | 417 | 43.86 | 7.31 | Chr4:193207888-193210831 | 1 |
Zm00001d053967 | ZmbZIP67 | 314 | 33.62 | 6.17 | Chr4:244414417-244418397 | 1 |
Zm00001d015118 | ZmbZIP73 | 290 | 31.01 | 5.22 | Chr5:76331375-76334353 | 1 |
Zm00001d039065 | ZmbZIP96 | 377 | 40.12 | 7.51 | Chr6:169491185-169496107 | 10 |
Zm00001d039206 | ZmbZIP97 | 208 | 22.36 | 6.84 | Chr6:172509105-172510659 | 1 |
Zm00001d019166 | ZmbZIP100 | 379 | 39.68 | 6.88 | Chr7:19967615-19972466 | 8 |
Zm00001d045288 | ZmbZIP123 | 547 | 60.82 | 7.95 | Chr9:18011858-18027409 | 1 |
Zm00001d047967 | ZmbZIP133 | 423 | 45.11 | 9.94 | Chr9:146611340-146618721 | 4 |
Zm00001d046664 | ZmbZIP136 | 187 | 20.02 | 8.56 | Chr10:5649486-5650525 | 1 |
Zm00001d024041 | ZmbZIP138 | 282 | 29.93 | 7.12 | Chr10:39073197-39078744 | 11 |
Zm00001d024285 | ZmbZIP140 | 213 | 23.6 | 6.03 | Chr10:61712124-61716615 | 1 |
Zm00001d025638 | ZmbZIP142 | 273 | 30.15 | 8.45 | Chr10:124742514-124747717 | 1 |
图1 ZmbZIP基因的染色体定位与基因结构 A,ZmbZIP基因的染色体定位;B,ZmbZIP的基因结构。
Fig.1 Chromosomal distribution and structure of ZmbZIP genes A, Chromosomal distribution of ZmbZIPs; B, Gene structure analysis of ZmbZIPs.
图2 ZmbZIP蛋白的保守结构域多重序列比对与进化树 A,ZmbZIP蛋白保守结构域多重序列比对;B,ZmbZIP蛋白的进化树。
Fig.2 Multiple sequence alignment of conserved domain of ZmbZIP proteins and their phylogenetic tree A, Multiple sequence alignment of conserved domain of ZmbZIP; B, Phylogenetic tree of ZmbZIPs.
图3 ZmbZIPs基因在玉米不同器官的表达模式 R、S、L、T、E分别代表根、茎、叶、雄花和幼穗。柱上无相同小写字母代表差异显著(P<0.05)。下同。
Fig.3 Expression pattern of ZmbZIPs in different organs of maize R, S, L, CB, T, E represent root, stem, leaf, corn brack and tassel, respectively. Bars marked without the same lowercase letter indicated significant differences at P<0.05. The same as below.
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