纳豆菌NT-6发酵患病畜禽肉骨粉制备微生物肥料

doi:10.3969/j.issn.1004-1524.2022.03.18

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (3): 574-581.DOI: 10.3969/j.issn.1004-1524.2022.03.18

纳豆菌NT-6发酵患病畜禽肉骨粉制备微生物肥料

唐晓山¹^,²(), 侯小琴², 孙力军²^,^*(), 房志家², 邓旗²

1.岭南师范学院分析测试中心,广东湛江 524048
2.广东海洋大学食品科技学院,广东湛江 524088

收稿日期:2020-10-09 出版日期:2022-03-25 发布日期:2022-03-30
通讯作者: 孙力军
作者简介:*孙力军,E-mail: suncamt@126.com
唐晓山(1977—),男,河北唐山人,硕士研究生,高级实验师,研究方向为食源微生物耐药机制。E-mail: 853940637@qq.com
基金资助:
广东省科技创新战略专项资金竞争性分配项目(2018A03010)

Production of bacterial manure via fermentation of meat and bone meals of sick animals by Bacillus natto NT-6

TANG Xiaoshan¹^,²(), HOU Xiaoqin², SUN Lijun²^,^*(), FANG Zhijia², DENG Qi²

1. Analysis and Testing Center, Lingnan Normal University, Zhanjiang 524048, Guangdong, China
2. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, Guangdong, China

Received:2020-10-09 Online:2022-03-25 Published:2022-03-30
Contact: SUN Lijun

摘要/Abstract

摘要：

以肉骨粉为主要发酵基质,以纳豆菌(Bacillus natto)NT-6作为发酵菌和功能菌进行固态发酵,制备微生物肥料。以菌量为指标,通过单因素试验和响应面设计确定最佳发酵条件,并在此基础上利用盆栽试验探索成品对作物茎基腐病病原菌——立枯丝核菌(Rhizoctonia solani Kühn)的抗性。结果显示,将肉骨粉与麸皮按照7.8:2.2的质量比混合,设定水分含量58.19%、稻壳添加量0.82 g、发酵时间100 h,菌量最高(2.92×10¹⁰ CFU·g^-1)。制得的微生物肥料对番茄种子发芽的促进作用不明显,但可提高番茄的生物量。在染菌园土上,番茄苗因茎基腐病导致的死苗率为55%,施用制得的微生物肥料后,番茄苗因茎基腐病导致的死苗率降至20%。结果说明,用纳豆菌NT-6发酵肉骨肥可以制作微生物肥,应用于番茄上,不仅可提高其生物量,而且对立枯丝核菌也具有明显的抗性。

关键词: 纳豆芽孢杆菌, 肉骨粉, 抗菌脂肽, 微生物肥料

Abstract:

In the present study, meat and bone meals were used as fermentation substrate, Bacillus natto NT-6 was used as fermentation and function bacteria, and bacterial manure was produced by soil fermentation.With NT-6 quantity as the main index, the fermentation conditions were optimized by single factor tests and response surface design. On the basis, the effect of the produced bacterial manure against pathogen of the tomato stem rot (Rhizoctonia solani Kühn) were explored. It was shown that the optimal fermentation condition were as follows: the ratio of meat bone to bran was 7.8:2.2, the moisture content was 58.19%, the addition amount of rice husks was 0.82 g, the fermentation time was 100 h. Under this condition, the NT-6 quantity in fermentation product was the highest (2.92×10¹⁰ CFU·g^-1). The produced bacterial manure had no significant promotion effect on seed germination, yet increased biomass of tomato. In soils infected by R. solani, the mortality rate of tomato was 55%, yet it decreased to 20% with application of the produced bacteria manure. In general, meat and bone meals could be used to produce bacteria manure via solid fermentation by B. natto NT-6. The produced bacterial manure not only could enhance the growth of tomato, but also could increase resistance to R. solani.

Key words: Bacillus natto, meat and bone meals, antibacterial peptides, microbial fertilizer

中图分类号:

S144

唐晓山, 侯小琴, 孙力军, 房志家, 邓旗. 纳豆菌NT-6发酵患病畜禽肉骨粉制备微生物肥料[J]. 浙江农业学报, 2022, 34(3): 574-581.

TANG Xiaoshan, HOU Xiaoqin, SUN Lijun, FANG Zhijia, DENG Qi. Production of bacterial manure via fermentation of meat and bone meals of sick animals by Bacillus natto NT-6[J]. Acta Agriculturae Zhejiangensis, 2022, 34(3): 574-581.

图/表 9

参考文献 13

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编号No.	A/%	B	C/g	D/h	Y₁/(10⁹ CFU·g^-1)	Y₂/(10⁹ CFU·g^-1)
1	60(0)	9:1(-1)	1.2(1)	96(0)	25.67	25.64
2	60(0)	9:1(-1)	0.8(0)	72(-1)	18.85	18.95
3	70(1)	8:2(0)	1.2(1)	96(0)	25.00	25.24
4	60(0)	8:2(0)	0.4(-1)	120(1)	20.90	20.95
5	60(0)	8:2(0)	0.8(0)	96(0)	29.13	29.05
6	70(1)	8:2(0)	0.8(0)	120(1)	20.93	20.94
7	60(0)	7:3(1)	0.8(0)	120(1)	23.48	23.39
8	60(0)	7:3(1)	0.8(0)	72(-1)	16.80	16.82
9	70(1)	7:3(1)	0.8(0)	96(0)	24.33	24.33
10	50(-1)	9:1(-1)	0.8(0)	96(0)	24.63	24.70
11	60(0)	9:1(-1)	0.8(0)	120(1)	20.80	20.80
12	60(0)	8:2(0)	0.8(0)	96(0)	29.05	29.05
13	60(0)	7:3(1)	0.4(-1)	96(0)	25.60	25.55
14	50(-1)	8:2(0)	0.8(0)	120(1)	22.40	22.41
15	50(-1)	7:3(1)	0.8(0)	96(0)	27.59	27.67
16	60(0)	8:2(0)	1.2(1)	72(-1)	17.05	17.06
17	70(1)	8:2(0)	0.4(-1)	96(0)	24.67	24.75
18	50(-1)	8:2(0)	0.8(0)	72(-1)	17.43	17.34
19	50(-1)	8:2(0)	1.2(1)	96(0)	25.74	25.68
20	60(0)	8:2(0)	0.8(0)	96(0)	29.08	29.05
21	60(0)	9:1(-1)	0.4(-1)	96(0)	25.48	25.36
22	60(0)	8:2(0)	0.4(-1)	72(-1)	17.28	17.32
23	70(1)	8:2(0)	0.8(0)	72(-1)	17.68	17.59
24	60(0)	8:2(0)	0.8(0)	96(0)	29.03	29.05
25	60(0)	7:3(1)	1.2(1)	96(0)	25.88	25.91
26	60(0)	8:2(0)	1.2(1)	120(1)	21.83	21.85
27	70(1)	9:1(-1)	0.8(0)	96(0)	26.85	26.83
28	50(-1)	8:2(0)	0.4(-1)	96(0)	25.32	25.31
29	60(0)	8:2(0)	0.8(0)	96(0)	28.98	29.05

编号No.	A/%	B	C/g	D/h	Y₁/(10⁹ CFU·g^-1)	Y₂/(10⁹ CFU·g^-1)
1	60(0)	9:1(-1)	1.2(1)	96(0)	25.67	25.64
2	60(0)	9:1(-1)	0.8(0)	72(-1)	18.85	18.95
3	70(1)	8:2(0)	1.2(1)	96(0)	25.00	25.24
4	60(0)	8:2(0)	0.4(-1)	120(1)	20.90	20.95
5	60(0)	8:2(0)	0.8(0)	96(0)	29.13	29.05
6	70(1)	8:2(0)	0.8(0)	120(1)	20.93	20.94
7	60(0)	7:3(1)	0.8(0)	120(1)	23.48	23.39
8	60(0)	7:3(1)	0.8(0)	72(-1)	16.80	16.82
9	70(1)	7:3(1)	0.8(0)	96(0)	24.33	24.33
10	50(-1)	9:1(-1)	0.8(0)	96(0)	24.63	24.70
11	60(0)	9:1(-1)	0.8(0)	120(1)	20.80	20.80
12	60(0)	8:2(0)	0.8(0)	96(0)	29.05	29.05
13	60(0)	7:3(1)	0.4(-1)	96(0)	25.60	25.55
14	50(-1)	8:2(0)	0.8(0)	120(1)	22.40	22.41
15	50(-1)	7:3(1)	0.8(0)	96(0)	27.59	27.67
16	60(0)	8:2(0)	1.2(1)	72(-1)	17.05	17.06
17	70(1)	8:2(0)	0.4(-1)	96(0)	24.67	24.75
18	50(-1)	8:2(0)	0.8(0)	72(-1)	17.43	17.34
19	50(-1)	8:2(0)	1.2(1)	96(0)	25.74	25.68
20	60(0)	8:2(0)	0.8(0)	96(0)	29.08	29.05
21	60(0)	9:1(-1)	0.4(-1)	96(0)	25.48	25.36
22	60(0)	8:2(0)	0.4(-1)	72(-1)	17.28	17.32
23	70(1)	8:2(0)	0.8(0)	72(-1)	17.68	17.59
24	60(0)	8:2(0)	0.8(0)	96(0)	29.03	29.05
25	60(0)	7:3(1)	1.2(1)	96(0)	25.88	25.91
26	60(0)	8:2(0)	1.2(1)	120(1)	21.83	21.85
27	70(1)	9:1(-1)	0.8(0)	96(0)	26.85	26.83
28	50(-1)	8:2(0)	0.4(-1)	96(0)	25.32	25.31
29	60(0)	8:2(0)	0.8(0)	96(0)	28.98	29.05

处理 Treatment	出芽数 Budding number	死苗数 Number of dead plants	株高 Plant height/mm	鲜重 Fresh weight/g
T1	17	3	178.3±5.19	1.86±0.42
T2	15	5	122.6±2.74	1.24±0.51
T3	16	4	163.2±4.21	1.65±0.19
T4	9	11	55.4±2.10	0.56±0.16

处理 Treatment	出芽数 Budding number	死苗数 Number of dead plants	株高 Plant height/mm	鲜重 Fresh weight/g
T1	17	3	178.3±5.19	1.86±0.42
T2	15	5	122.6±2.74	1.24±0.51
T3	16	4	163.2±4.21	1.65±0.19
T4	9	11	55.4±2.10	0.56±0.16

纳豆菌NT-6发酵患病畜禽肉骨粉制备微生物肥料

Production of bacterial manure via fermentation of meat and bone meals of sick animals by Bacillus natto NT-6

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