铝在茶树叶片亚细胞中的分布及其与细胞壁的结合研究

doi:10.3969/j.issn.1004-1524.2023.03.03

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (3): 509-514.DOI: 10.3969/j.issn.1004-1524.2023.03.03

铝在茶树叶片亚细胞中的分布及其与细胞壁的结合研究

李春雷¹(), 徐红梅¹, 刘杰¹, 张汝君¹, 马兴云¹, 张华²

1.潍坊科技学院农学院,山东省设施园艺生物工程研究中心,山东寿光 262700
2.山东农业大学园艺科学与工程学院,山东泰安 271018

收稿日期:2022-04-29 出版日期:2023-03-25 发布日期:2023-04-07
作者简介:李春雷(1979—),男,山东潍坊人,博士,教授,主要从事茶树栽培生理与生化方面的研究。E-mail: lcl432@163.com
基金资助:
国家自然科学基金(31400585);潍坊市科技发展计划(2021GX049)

Aluminum subcellular distribution and its combining characreristics with cell wall in tea leaves

LI Chunlei¹(), XU Hongmei¹, LIU Jie¹, ZHANG Rujun¹, MA Xingyun¹, ZHANG Hua²

1. Shandong Facility Horticulture Bioengineering Research Center, College of Agronomy, Weifang University of Science and Technology, Shouguang 262700, Shandong, China
2. College of Horticultural Science and Engineering, Shandong Agricultural University,Tai’an 271018, Shandong, China

Received:2022-04-29 Online:2023-03-25 Published:2023-04-07

摘要/Abstract

摘要：

茶树是一种非常典型的聚铝植物,主要富集在叶片中。为了解析茶树叶片聚铝的特性,以高铝品种农抗早和低铝品种平阳特早的成熟叶片为材料,分析了茶树叶片中铝的亚细胞分布及与细胞壁可能的结合方式。结果表明,两个品种(农抗早、平阳特早)叶片中的铝主要分布在细胞壁中(46.2%~54.9%),其次是细胞核和叶绿体(24.2%~31.2%)。在细胞壁组分中,果胶是细胞壁富集铝的主要组分,占细胞壁中铝含量的60.1%~79.0%。细胞壁经甲基改性处理和酯化改性处理均显著降低了铝含量,而纤维素酶和果胶酶酶解处理对细胞壁中铝含量影响较小,说明细胞壁中的羧基和氨基在结合铝上起着关键性作用,而糖分子链长短对铝的结合作用不明显。因此,茶树叶片细胞壁是铝聚集的主要部位,阻挡了铝进一步进入细胞内部,降低了铝的毒害作用;铝很可能以氢键的形式与细胞壁中羧基和氨基结合而被固定在细胞壁中。

关键词: 茶树, 铝, 细胞壁, 亚细胞, 结合特性

Abstract:

Tea tree (Camellia sinensis) is a very typical aluminium (Al) concentrating plant, which is mainly enriched in the leaves. In order to analyze the characteristics of Al content in tea leaves, this paper explored the accumulation characteristics of Al in tea leaves using mature leaves of the high-Al variety Nongkangzao and the low-Al variety Pingyangtezao as materials, and analyzed the subcellular distribution of Al in tea leaves and the possible binding mode with the cell wall. The results showed that Al in the leaves of the two varieties (Nongkangzao and Pingyangtezao) was mainly distributed in the cell wall (46.2%-54.9%), and to a less extent in the nucleus and chloroplasts (24.2%-31.2%). In the cell wall fractions, pectin was the main Al-enriched component of the cell wall, accounting for 60.1%-79.0% of the Al content in the cell wall. Both methyl-modified and esterified treatments significantly reduced the Al content of the cell wall, while the cellulase and pectinase enzymatic treatments had less effect on the Al content of the cell wall, suggesting that the -COOH and -NH₂ in the cell wall play a key role in binding Al, while the length of the sugar molecule chains had no significant effect on the Al binding. Therefore, the cell wall of tea leaves is the main location where Al accumulates, preventing it from entering further into the cell interior and reducing its toxic effects; Al is most probably bound to the carboxyl and amino groups in the cell wall in the form of a hydrogen bond and is immobilised in the cell wall.

Key words: Camellia sinensis, aluminum, cell wall, subcellular distribution, combining characteristics

中图分类号:

S571.1

李春雷, 徐红梅, 刘杰, 张汝君, 马兴云, 张华. 铝在茶树叶片亚细胞中的分布及其与细胞壁的结合研究[J]. 浙江农业学报, 2023, 35(3): 509-514.

LI Chunlei, XU Hongmei, LIU Jie, ZHANG Rujun, MA Xingyun, ZHANG Hua. Aluminum subcellular distribution and its combining characreristics with cell wall in tea leaves[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 509-514.

图/表 7

图1 铝在茶树叶片亚细胞中的分布图中同一品种没有相同小写字母表示差异显著(P<0.05),下同。

Fig.1 Subcellular distribution of Al in tea plant leaves Different lowercase letters in the same cultivar indicate a significant difference at P<0.05. The same as below.

图2 平阳特早茶树叶片中铝亚细胞分布的相对比例

Fig.2 Relative proportion of Al subcellular distribution in tea plant leaves of Pingyangtezao

图3 农抗早茶树叶片中铝亚细胞分布的相对比例

Fig.3 Relative proportion of Al subcellular distribution in tea plant leaves of Nongkangzao

图4 铝在茶树叶片细胞壁各组分的分布

Fig.4 Al distribution in cell wall fractions in tea plant leaves

图5 平阳特早茶树叶片细胞壁各组分中铝分布的相对比例

Fig.5 Relative Al percentages in cell wall fractions of Pingyangtezao

图6 农抗早茶树叶片细胞壁各组分中铝分布的相对比例

Fig.6 Relative percentages of Al in cell wall fractions of Nongkangzao

图7 细胞壁改性后铝含量变化图中同一品种同一处理不同小写字母表示差异显著(P<0.05)。

Fig.7 The Al content of cell wall with methlation modification Different lowercase letters between the same cultivar and the same treatment indicate a significant difference at P<0.05.

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铝在茶树叶片亚细胞中的分布及其与细胞壁的结合研究

Aluminum subcellular distribution and its combining characreristics with cell wall in tea leaves

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