浙江农业学报 ›› 2025, Vol. 37 ›› Issue (4): 767-778.DOI: 10.3969/j.issn.1004-1524.20240173

• 作物科学 • 上一篇    下一篇

甘薯对低温胁迫的生理响应及耐寒性评价

刘思彤1,#(), 侯宇2,#(), 潘家荃1, 周桦楠1, 崔亮1, 万博1, 于涛1,*()   

  1. 1.辽宁省农业科学院 作物研究所,辽宁 沈阳 110161
    2.辽宁省农业发展服务中心,辽宁 沈阳 110001
  • 收稿日期:2024-02-26 出版日期:2025-04-25 发布日期:2025-05-09
  • 作者简介:刘思彤(1995—),女,辽宁北镇人,满族,硕士,从事薯类育种与栽培研究。E-mail:1519150658@qq.com
    侯宇(1979—),男,辽宁沈阳人,学士,工程师,从事薯类育种与栽培研究。E-mail:13840453327@163.com第一联系人:#为共同第一作者
  • 通讯作者: *于涛,E-mail: taoyu@cau.edu.cn
  • 基金资助:
    沈阳市种业创新专项(22-318-2-18);辽宁省农业科学院院长基金项目(2024QN1806);辽宁省农业科学院院长基金项目(2023QN2423);甘薯种质创新应用合作协议(2022210101001261)

Physiological response of sweetpotato to the low temperature and evaluation of cold tolerance

LIU Sitong1(), HOU Yu2(), PAN Jiaquan1, ZHOU Huanan1, CUI Liang1, WAN Bo1, YU Tao1,*()   

  1. 1. Crop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China
    2. Liaoning Provincial Agricultural Development Service Center, Shenyang 110001, China
  • Received:2024-02-26 Online:2025-04-25 Published:2025-05-09

摘要: 本研究对84个甘薯品种进行耐寒性鉴定,测定分析4 ℃低温胁迫下薯苗存活率、相对电导率、抗氧化酶活性和渗透调节物质含量,利用主成分分析、聚类分析等统计学方法进行综合评价。结果表明,4 ℃低温胁迫时,不同地区选育的甘薯品种间耐寒性存在显著差异,耐寒性强的品种薯苗存活率显著高于耐寒性弱的品种,其中,辽寒薯21薯苗存活率最高,为81.1%,其次为徐薯33,薯苗存活率72.5%;辽寒薯21和徐薯33抗氧化酶活性和渗透调节物质含量在胁迫前后(0、48 h)均高于苏薯28和万薯7号;相关性分析和主成分分析表明,SOD活性、POD活性、CAT活性和脯氨酸含量与薯苗存活率呈显著正相关关系。综合分析认为,辽寒薯21和徐薯33耐寒性最强,苏薯28和万薯7号耐寒性最弱。

关键词: 甘薯, 耐寒性, 生理特征, 主成分分析, 聚类分析

Abstract:

Eighty-four sweetpotato varieties were identified for cold tolerance, and the survival rate, relative conductivity, antioxidant enzyme activity and osmoregulatory substance content under low-temperature stress at 4 ℃ were determined and analyzed, and comprehensive evaluation was carried out using statistical methods such as principal component analysis and cluster analysis. The results showed that there were significant differences in cold tolerance among sweetpotato varieties selected from different regions during low-temperature stress at 4 ℃, the survival rate of sweetpotato seedlings of the cold-tolerant varieties was significantly higher than that of the weakly cold-tolerant varieties after 4 ℃ low-temperature stress, the survival rate of sweetpotato seedlings of Liaohanshu 21 was the highest(81.1%), followed by Xushu 33(72.5%). The antioxidant enzyme activities and osmolyte contents of Liaohanshu 21 and Xushu 33 were higher than Sushu 28 and Wanshu No.7 before and after stress (0 h and 48 h). Correlation analysis and principal component analysis showed that SOD activity, POD activity, CAT activity and proline content were significantly positively correlated with the survival rate of potato seedlings, and the comprehensive analysis showed that Liaohanshu 21 and Xushu 33 were the most cold-tolerant, while Sushu 28 and Wanshu No.7 were the least cold-tolerant.

Key words: sweetpotato, cold tolerance, physiological characteristics, principal component analysis, cluster analysis

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