浙江农业学报 ›› 2017, Vol. 29 ›› Issue (8): 1290-1296.DOI: 10.3969/j.issn.1004-1524.2017.08.09

• 动物科学 • 上一篇    下一篇

静原鸡ELOVL2和ELOVL5基因表达的组织特异性研究

母童, 张娟*, 赵平, 顾亚玲, 刘丽元, 杨彦军, 安克龙, 王有   

  1. 宁夏大学 农学院,宁夏 银川 750021
  • 收稿日期:2017-01-12 出版日期:2017-08-20 发布日期:2017-09-06
  • 通讯作者: 张娟,E-mail: zhangjkathy@126.com
  • 作者简介:母童(1995-),男,宁夏银川人,硕士研究生,主要从事动物遗传育种与繁殖方面的研究。E-mail: 1227415848@qq.com
  • 基金资助:
    宁夏自然科学基金资助项目(NZ15011)

Tissue-specific expression analysis of ELOVL2 and ELOVL5 genes in Jingyuan chicken

MU Tong, ZHANG Juan*, ZHAO Ping, GU Yaling, LIU Liyuan, YANG Yanjun, AN Kelong, WANG You   

  1. College of Agriculture, Ningxia University, Yinchuan 750021, China
  • Received:2017-01-12 Online:2017-08-20 Published:2017-09-06

摘要: 采用实时荧光定量PCR(qRT-PCR)技术对宁夏地方优良品种静原鸡的肝脏、肌肉、心脏、睾丸、卵巢、腹脂6个组织中ELOVL2和ELOVL5基因的表达量进行了测定,并通过SAS8.2软件进行分析统计。结果表明,ELOVL2和ELOVL5基因在静原鸡6个不同组织中均有表达。其中ELOVL2基因在肝脏中的相对表达量最高,为71.52±0.41,显著高于其他组织(P<0.05);其次是卵巢、睾丸、腹脂、心脏及肌肉,在另外5个组织中表达量虽有差异,但均差异不显著(P>0.05)。ELOVL5基因在静原鸡不同组织中的相对表达量依次为:肝脏>腹脂>卵巢>睾丸>心脏>肌肉,同样在肝脏中的表达量最高,为110.94±0.02,显著高于其他组织(P<0.05),在腹脂中的表达量显著高于卵巢、睾丸、心脏和肌肉(P<0.05)。另外在心脏和肌肉中的表达量均差异不显著(P>0.05)。对ELOVL2和ELOVL5基因分别在不同组织中的表达差异进行分析比较,发现两个基因在腹脂中的表达量差异显著(P<0.05),ELOVL5基因在腹脂中的表达量较高。研究结果说明,ELOVL2和ELOVL5基因在静原鸡6种组织中的表达存在差异,并以肝脏组织最为明显,这为地方品种的开发与利用、种质资源遗传评定及地方品种分子育种技术平台的构建奠定了一定的理论基础。

关键词: 静原鸡, ELOVL2, ELOVL5, 相对表达量

Abstract: By using real-time quantitative PCR (qRT-PCR) expression of ELOVL2 and ELOVL5 in six different tissues (liver, muscle, heart, testis, ovary, abdominal fat) of Ningxia local varieties of Jingyuan chicken were determined, and the statistical analysis was carried out by SAS 8.2 software. The results showed that the expression of ELOVL2 and ELOVL5 genes were observed in six different tissues from Jingyuan chicken. The relative expression of ELOVL2 gene in liver was the highest, 71.52±0.41, significantly higher than that in other tissues (P<0.05), followed by the ovaries, testicles, abdominal fat, heart and muscle. There was a difference between the latter five tissues, but the difference was not significant (P>0.05). The relative expression of ELOVL5 gene in different tissues of Jingyuan chicken was as followed: liver>abdominal fat>ovary>testicle>heart>muscle. The highest expression level in liver was 110.94±0.02, which was significantly higher than that in other tissues (P<0.05), and the expression in abdominal fat was significantly higher than that in ovary, testis, heart and muscle (P<0.05). In addition, there was no significant difference in the expression in heart and muscle (P>0.05). The expression of ELOVL2 and ELOVL5 genes in different tissues was analyzed and compared. The results showed that the expression of two genes in abdominal fat was significantly different (P<0.05), and the expression of ELOVL5 gene in abdominal fat was higher. The research results showed that there were differences in the expression of ELOVL2 and ELOVL5 genes in six tissues from Jingyuan chicken, and liver tissue was the most obvious, which lays the foundation for the development of local technology platform to build local varieties of molecular breeding and genetic evaluation of germplasm resources.

Key words: Jingyuan chicken, ELOVL2, ELOVL5, relative expression

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