关键词: 土党参, 愈伤组织, 丛生芽, 生根率, 成活率

Abstract: In order to estabilished an efficient plant regeneration system via callus from stem segments with bud of Campanumoea javanica B1. The aseptic stems were used as explants and then cultured on MS medium supplemented with different plant growth regulators (PGRs) to establish an effective protocol for callus induction, shoots induction and development, and roots induction. The results showed that two types of callus could be obtained under different treatments, of which type Ⅰ was yellow and compact while type Ⅱ was light yellow, fragile and mixed with a small number of white globular embryos. The type of callus could be regulated by cytokinin. Type I callus was induced on MS medium supplemented with 1.0 mg·L^-1 2, 4-D+1.0 mg·L^-1 6-BA + 0.5 mg·L^-1 NAA, while type Ⅱ callus was induced on MS medium supplemented with 1.0 mg·L^-1 2, 4-D. The compact callus could differentiated into adventitious buds on the medium supplementing cytokinin (6-BA, KT, TDZ). The adventitious buds further developed and proliferated after 30 d culture on MS medium containing 0.5 mg·L^-1 6-BA+0.5 mg·L^-1 GA₃+0.1 mg·L^-1 NAA. Excised shoots were treated with 300 mg·L^-1 IBA for 15 min and then cultured on MS basal medium for 30 d, and the rooting rate reached 100%. When transplanted into a mixture of peat soil, vermiculite and perlite (volume ratios was 1∶1∶1), the survival rate of regenerated plantlets could reached 73.3% after 30 d. The experiment developed an effective protocol for C. javanica shoot organogenesis via callus, which might laid the foundation of researches on polyploidy, transgenic and cell mutation breeding.

Key words: Campanumoea javanica B1., callus, excised shoots, rooting rate, survival rate