长时间镁胁迫对芹菜叶绿素荧光特性与抗氧化能力的影响

doi:10.3969/j.issn.1004-1524.20230022

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (2): 295-307.DOI: 10.3969/j.issn.1004-1524.20230022

长时间镁胁迫对芹菜叶绿素荧光特性与抗氧化能力的影响

虎丽霞(), 张婧, 高彦强, 毛尔晔, 韩康宁, 杨滟, 颉建明^*()

甘肃农业大学园艺学院,甘肃兰州 730070

收稿日期:2022-01-11 出版日期:2024-02-25 发布日期:2024-03-05
作者简介:虎丽霞(1996—),女,甘肃岷县人,硕士研究生,研究方向为设施蔬菜栽培生理与生长调控。E-mail:2655853640@qq.com
通讯作者: *颉建明,E-mail:xiejianming@gsau.edu.cn
基金资助:
国家重点研发计划(2016YFD0201005);甘肃省瓜菜产业技术体系专项资金(GARS-GC-1);甘肃省科技计划(21JR7RA821)

Effects of long-term magnesium stress on chlorophyll fluorescence characteristics and antioxidant properties of celery

HU Lixia(), ZHANG Jing, GAO Yanqiang, MAO Erye, HAN Kangning, YANG Yan, XIE Jianming^*()

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China

Received:2022-01-11 Online:2024-02-25 Published:2024-03-05

摘要/Abstract

摘要：

为研究长时间镁缺乏和镁过量胁迫对芹菜叶绿素荧光特性和抗氧化系统的影响,以美国西芹为材料,采用水培方式,以日本山崎全营养液为对照(CK,1.0 mmol·L^-1 Mg²⁺),设镁缺乏(0、0.5 mmol·L^-1)和镁过量(2.5 mmol·L^-1)处理,处理65 d后测定芹菜的叶绿素含量、叶绿素荧光参数和抗氧化相关指标。结果表明:镁缺乏和镁过量胁迫均导致芹菜叶片的叶绿素含量、PSⅡ最大光化学效率(F_v/F_m)、光化学猝灭系数(qP)、光化学猝灭(qL)、光合电子传递速率(ETR)、单位反应中心吸收光能(ABS/RC)、单位面积吸收的光能(ABS/CSm)显著降低,J点相对可变荧光(V_j)、单位反应中心耗散能量(DIo/RC)、单位面积热耗散能量(DIo/CSm)均显著升高,OJIP曲线发生明显变形;镁过量胁迫导致以吸收光能为基础的性能指数(PI abs)、从Q_A到Q_B的电子传输通量的量子产率(φE_o)均显著降低,非光化学猝灭系数(qN)显著升高。镁过量胁迫时芹菜叶片的苯丙氨酸解氨酶(PAL)、肉桂酸羟化酶(C4H)和4-香豆酰-辅酶A连接酶(4CL)的活性均显著增强,类黄酮含量显著升高。镁缺乏和镁过量胁迫时芹菜叶片和叶柄的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性均显著降低,丙二醛(MDA)、脯氨酸(Pro)和过氧化氢(H₂O₂)含量均显著升高。镁缺乏胁迫时芹菜叶片的过氧化物酶(POD)活性显著增强,超氧阴离子含量显著降低。镁过量胁迫对芹菜叶绿素荧光特性和抗氧化能力的影响显著大于镁缺乏胁迫。

关键词: 芹菜, 镁, 叶绿素荧光, 酚类代谢, 抗氧化酶

Abstract:

In order to study the effects of long-term magnesium deficiency and magnesium excess stress on chlorophyll fluorescence characteristics and antioxidant system of celery, American celery was used as the material, and the Yamazaki total nutrient solution was used as the control (CK, 1.0 mmol·L^-1 Mg²⁺), magnesium deficiency (0, 0.5 mmol·L^-1) and magnesium excess (2.5 mmol·L^-1) treatments were set. After 65 days of treatment, the chlorophyll content, chlorophyll fluorescence parameters and antioxidant related indicators of celery were determined. The results showed that the chlorophyll content, the maximum photochemical efficiency (F_v/F_m) of PSⅡ, the photochemical quenching coefficient (qP), the photochemical quenching (qL), the photosynthetic electron transfer rate (ETR), the light energy absorbed per unit reaction center (ABS/RC) and the light energy absorbed per unit area (ABS/CSm) of celery leaves were significantly reduced by magnesium deficiency and magnesium excess stress, while the relative variable fluorescence (V_j) of J point, the dissipated energy per unit reaction center (DIo/RC), the thermal dissipation energy per unit area (DIo/CSm) were significantly increased, and the OJIP curve was significantly deformed. Excessive magnesium leads to the performance index (PI abs) based on the absorption of light energy, and the quantum yield of the electron transport flux from Q_A to Q_B (φEo) significantly decreased, and non-photochemical quenching (qN) significantly increased. The activities of phenylalanine ammonia-lyase (PAL), cinnamate hydroxylase (C4H), 4-coumaryl coenzyme A ligase (4CL) and the content of flavonoids in celery leaves were significantly increased under magnesium excess stress. The activities of superoxide dismutase (SOD), catalase (CAT) and ascorbic acid peroxidase (APX) in celery leaves and petioles decreased significantly under deficiency and excess stress of magnesium, while the contents of malondialdehyde (MDA), proline (Pro) and hydrogen peroxide (H₂O₂) increased significantly. The peroxidase (POD) activity of celery leaves was significantly increased and the content of superoxide anion was significantly decreased under magnesium deficiency stress. The effect of magnesium excess stress on chlorophyll fluorescence characteristics and antioxidant capacity of celery was significantly greater than that of magnesium deficiency stress.

Key words: celery, magnesium, chlorophyll fluorescence, phenolic metabolism, antioxidase

中图分类号:

S636.3

虎丽霞, 张婧, 高彦强, 毛尔晔, 韩康宁, 杨滟, 颉建明. 长时间镁胁迫对芹菜叶绿素荧光特性与抗氧化能力的影响[J]. 浙江农业学报, 2024, 36(2): 295-307.

HU Lixia, ZHANG Jing, GAO Yanqiang, MAO Erye, HAN Kangning, YANG Yan, XIE Jianming. Effects of long-term magnesium stress on chlorophyll fluorescence characteristics and antioxidant properties of celery[J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 295-307.

图/表 12

图1 镁胁迫处理65 d的芹菜叶片生长形态

Fig.1 Growth morphology of celery leaves treated under magnesium stress for 65 days

图2 镁胁迫对芹菜叶片叶绿素含量的影响 Chla、Chlb、Chl(a+b)分别为叶绿素a、叶绿素b和叶绿素a+b。柱状图上无相同小写字母的表示各处理间差异显著(P<0.05)。

Fig.2 Effects of magnesium stress on chlorophyll content of celery leaves Chla, Chlb and Chl (a+b) are chlorophyll a, chlorophyll b and chlorophyll (a+b), respectively. Different lowercase letters above the bars represent significant differences (P<0.05) among treatments.

表1 镁胁迫下芹菜叶片的叶绿素荧光参数

Table 1 Chlorophyll fluorescence parameters of celery leaves under magnesium stress

MgSO₄·7H₂O浓度 MgSO₄·7H₂O concentration/(mmol·L^-1)	F_v/F_m	Y(Ⅱ)	Y(NPQ)	NPQ	qN	qP	qL	ETR
0	0.719 ±0.001 c	0.578 ±0.003 a	0.144 ±0.002 a	0.129 ±0.002 a	0.451 ±0.005 ab	0.850 ±0.014 b	0.638 ±0.024 c	17.467 ±0.533 c
0.5	0.727 ±0.001 b	0.582 ±0.002 a	0.138 ±0.005 a	0.124 ±0.005 a	0.437 ±0.012 ab	0.869 ±0.012 b	0.694 ±0.020 bc	21.667 ±0.167 b
1.0 (CK)	0.752 ±0.001 a	0.590 ±0.005 a	0.115 ±0.010 a	0.095 ±0.009 a	0.356 ±0.023 b	0.920 ±0.005 a	0.811 ±0.009 a	24.600 ±0.208 a
2.5	0.729 ±0.003 b	0.485 ±0.017 b	0.190 ±0.035 a	0.176 ±0.036 a	0.523 ±0.067 a	0.872 ±0.010 b	0.726 ±0.012 b	18.267 ±0.775 c

图3 镁胁迫下芹菜叶片的叶绿素荧光动力学曲线

Fig.3 Chlorophyll fluorescence kinetics curve of celery leaves under magnesium stress

表2 镁胁迫下芹菜叶片的JIP-test参数

Table 2 JIP-test parameters of celery leaves under magnesium stress

MgSO₄·7H₂O浓度 MgSO₄·7H₂O concentration/(mmol·L^-1)	V_i	V_j	S_m	PI abs	φP_o	φE_o	φR_o	dV/dto
0	0.903 ±0.087 a	0.579 ±0.004 a	17.221 ±0.920 a	1.783 ±0.082 ab	0.851 ±0.004 a	0.358 ±0.004 a	0.082 ±0.007 b	1.144 ±0.017 a
0.5	0.875 ±0.005 ab	0.582 ±0.009 a	18.144 ±0.608 a	1.900 ±0.055 a	0.840 ±0.003 a	0.363 ±0.015 a	0.105 ±0.004 ab	1.140 ±0.065 a
1.0 (CK)	0.865 ±0.008 b	0.543 ±0.006 b	18.706 ±0.562 a	1.980 ±0.107 a	0.843 ±0.002 a	0.385 ±0.005 a	0.113 ±0.006 a	1.046 ±0.035 a
2.5	0.894 ±0.006 ab	0.606 ±0.007 a	15.936 ±0.529 a	1.480 ±0.079 b	0.844 ±0.004 a	0.320 ±0.007 b	0.090 ±0.005 ab	1.223 ±0.040 a

表3 镁胁迫下芹菜叶片PSⅡ的单个反应活性中心能量分配和单位截面积能量分配

Table 3 Energy allocation of single reactive active center and energy allocation per unit cross-sectional area of PSⅡ in celery leaves under magnesium stress

MgSO₄·7H₂O浓度 MgSO₄·7H₂O concentration/(mmol·L^-1)	ABS/RC	DIo/RC	TRo/RC	ETo/RC	ABS/CSm	DIo/CSm	TRo/CSm	ETo/CSm
0	2.307 ±0.014 b	0.379 ±0.004 a	1.965 ±0.010 ab	0.838 ±0.011 a	3 144 ±45.764 b	527 ±4.410 a	2 664 ±45.622 a	1 145 ±29.008 a
0.5	2.293 ±0.032 b	0.374 ±0.001 a	1.919 ±0.031 b	0.864 ±0.019 a	3 183 ±38.886 b	492 ±6.557 b	2 687 ±49.319 a	1 184 ±64.506 a
1.0 (CK)	2.395 ±0.007 a	0.343 ±0.005 b	2.041 ±0.034 a	0.882 ±0.013 a	3 373 ±66.419 a	472 ±3.512 b	2 784 ±68.280 a	1 254 ±25.829 a
2.5	2.308 ±0.026 b	0.381 ±0.004 a	1.980 ±0.027 ab	0.829 ±0.054 a	3 138 ±71.445 b	542 ±10.333 a	2 657 ±66.494 a	1 113 ±93.512 a

图4 镁胁迫对芹菜总酚和类黄酮含量的影响数据以鲜重计。柱状图上无相同小写字母的表示各处理间差异显著(P<0.05)。下同。

Fig.4 Effects of magnesium stress on the contents of total phenols and flavonoids in celery Data was detected based on fresh weight. Different lowercase letters above the bars represent significant differences (P<0.05) among treatments. The same as below.

图5 镁胁迫下芹菜中酚类代谢关键酶的活性

Fig.5 Key enzymes activities of phenolic metabolism in celery under magnesium stress

图6 镁胁迫下芹菜的丙二醛与脯氨酸含量

Fig.6 Contents of malondialdehyde and proline in celery under magnesium stress

图7 镁胁迫下芹菜的超氧阴离子含量

Fig.7 Superoxide anion content in celery under magnesium stress

图8 镁胁迫下芹菜的过氧化氢含量

Fig.8 Hydrogen peroxide content in celery under magnesium stress

图9 镁胁迫下芹菜的抗氧化酶活性

Fig.9 Antioxidant enzyme activities of celery under magnesium stress

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长时间镁胁迫对芹菜叶绿素荧光特性与抗氧化能力的影响

Effects of long-term magnesium stress on chlorophyll fluorescence characteristics and antioxidant properties of celery

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