›› 2017, Vol. 29 ›› Issue (9): 1524-1530.DOI: 10.3969/j.issn.1004-1524.2017.09.14

• Horticultural Science • Previous Articles     Next Articles

Genetic diversity and fingerprints of Gleditsia sinensis germplasm based on SRAP

ZHANG Anshi1, ZHANG Sumin2, FAN Dingchen3, LIU Ying1   

  1. 1. School of Science, Jiaozuo Teachers College, Jiaozuo 454000, China;
    2. Garden Conserving Institue of Jiaozuo, Jiaozuo 454000, China;
    3. Henan Academy of Forestry, Zhengzhou 450008, China
  • Received:2017-03-27 Online:2017-09-20 Published:2017-09-27

Abstract: Genetic diversity of 18 Gleditsia sinensis germplasms were analyzed by SRAP markers. The results showed that 17 primers pairs were screened from 64 primer pairs and 222 bands were obtained, including 213 polymorphic bands, with a polymorphism rate of 95.95%. The average polymorphism information content (PIC), observed number of alleles (Na), effective number of alleles (Ne), Nei's gene diversity (H) and Shannon's information index (I) were 0.861 1, 1.962 3, 1.415 3,0.257 2 and 0.406 6, and the genetic similarity coefficients (GS) among the tested samples ranged from 0.522 5 to 0.955 0. UPGMA analysis showed that 18 Gleditsia sinensis could clustered into 3 groups with the GS of 0.66. Gleditsia heterophylla formed the first group, Gleditsia melanacantha and Zaojia-T were classified into the second group, and the others were classified into the third group. A total of 18 Gleditsia sinensis germplasm DNA fingerprints were constructed from 9 polymorphic loci amplified by 4 primer pairs, and these materials could be distinguished and identified accurately. All these results would provide the important theoretical basis for the identification, conservation and breeding of new cultivar for Gleditsia sinensis germplasms.

Key words: Gleditsia sinensis, SRAP, DNA fingerprint, genetic diversity

CLC Number: