Study on biological characteristics of Lactobacillus from giant panda vagina

doi:10.3969/j.issn.1004-1524.2017.07.06

›› 2017, Vol. 29 ›› Issue (7): 1093-1102.DOI: 10.3969/j.issn.1004-1524.2017.07.06

• Animal Science • Previous Articles Next Articles

Study on biological characteristics of Lactobacillus from giant panda vagina

MA Xiaoping¹, YANG Tianyi¹, ZHANG Zhihe^{1, *}, YU Yan¹, WANG Chengdong³, GU Yu^{4, *}

1. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Chengdu Research Base of Giant Panda Breeding, Chengdu 610000, China;
3. China Conservation and Research Center for the Giant Panda, Ya??an 625000, China;
4. College of Life Science, Sichuan Agricultural University, Ya??an 625014, China

Received:2016-07-25 Online:2017-07-20 Published:2017-07-24

Abstract

Abstract: In the present study, 3 Lactobacillus strains of giant panda vagina (Lactobacillus salivarius GPV01, Lactobacillus plantarum GPV02 and Lactobacillus saerimneri GPV03) were used as experimental group, and 3 Lactobacillus strains of woman vagina (Lactobacillus acidophilus WAV01, Lactobacillus crispatus WAV02 and Lactobacillus plantarum WAV03) were used as control group to explore the difference of these strains in hydrogen peroxide production capacity, acid resistance, heat resistance, antibacterial activity, drug sensitivity and adhesiveness. It was shown that L. acidophilus WAV01, L. crispatus WAV02 and L. plantarum WAV03 possessed higher H₂O₂-producing ability and heat-resistance than L. salivarius GPV01, L. plantarum GPV02 and L. saerimneri GPV03 under the same culture condition. L. salivarius GPV01, L. plantarum GPV02 and L. plantarum WAV03 exhibited acid tolerance. D values of Lactobacillus suspensions were all between 0.2 to 0.55 for these strains when the pH was between 2 to 3 in the culture medium, and among these strains, L. plantarum GPV02 showed the highest D value (D=0.55) under the above condition. Six culture supernatants of the Lactobacillus strains had different inhibitory effects on Escherichia coli, Staphylococcus aureus and Hemolytic streptococcus. Six Lactobacillus strains showed weaker inhibition on three pathogenic bacteria than lactic acid (pH 3.5). Six Lactobacillus strains were highly sensitive to doxycycline, ampicillin, ceftriaxone, cefotaxime, amoxicillin, and were sensitive to tetracycline and rifampicin. Six Lactobacillus strains showed resistance to streptomycin, ofloxacin, gentamycin, selectrin, ciprofloxacin and sulfamethoxazole. Six Lactobacillus strains could stick on Hela, and the average adhesion number of GPV01, GPV03, WAV01 and WAV03 on each Hela cell was 18.8-32.8. WAV02 had the strongest adhesion ability (54.75±2.40)·cell^-1, while GPV02 had the worst adhesion ability (6.83±0.33)·cell^-1.

Key words: giant panda, Lactobacillus, biological characteristics

CLC Number:

MA Xiaoping, YANG Tianyi, ZHANG Zhihe, YU Yan, WANG Chengdong, GU Yu. Study on biological characteristics of Lactobacillus from giant panda vagina[J]. , 2017, 29(7): 1093-1102.

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