Genetic and QTL analysis of seed traits in melon (Cucumis melon L.)

doi:10.3969/j.issn.1004-1524.2018.09.08

›› 2018, Vol. 30 ›› Issue (9): 1496-1503.DOI: 10.3969/j.issn.1004-1524.2018.09.08

• Horticultural Science • Previous Articles Next Articles

Genetic and QTL analysis of seed traits in melon (Cucumis melon L.)

ZHANG Kexin¹, DAI Dongyang^{1, 2}, WANG Haonan¹, YU Mingyue¹, SHENG Yunyan^{1, *}

1. Agronomy College, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
2. Agricultural College, Shihezi University, Shihezi 832003, China

Received:2017-09-19 Online:2018-09-25 Published:2018-10-15
Contact: 盛云燕,E-mail: shengyunyan12345@163.com
Supported by:
国家自然科学基金(31401892,31772330); 黑龙江省自然科学基金(C2017054); 黑龙江省博士后科研启动项目; 黑龙江八一农垦大学大学生创新创业项目(XC2016001)

Abstract

Abstract: Crossing of F_2∶3 was made from cantaloupe ms5 and muskmelon HM1-1 as parental to study the genetic inheritance of seed related traits by Major Gene and Poly-gene Genetic Model, and genetic map and QTL analysis were conducted by F_2∶3 families. The results indicated that seed coat color (SCC) was controlled by a single dominance gene, and white dominated to yellow. 100 seed weight (SW) and seed diameter (SD) were quantitative traits fit for the A-1 model, which was under one additive-dominance single major gene control. Seed length (SL) fitted the B-1 model, which was controlled by two additive-dominance major genes. By using F₂ generation, a genetic map containing 153 CAPS (cleaved amplified polymorphism suquences) markers was constructed, which covered 1 104.2 cM in melon genome, and the average genetic distance was 7.2 cM. The SCC gene was mapped on the 5 linkage group, and the interval makers were HD0520 and HD0519 with the distance of 13.3 cM and 7.0 cM, respectively. SW was mapped on the linkage group 6 and 11, with sw6.1 linked to E0615 and E0618, and sw11.1 linked to E1113 and P1117. SL located on the linkage group 7 and 11, with sl7.1 linked to E0716 and HD0713, and sl11.1 and sl11.2 linked to makers E1110 and E1112, XB1114 and E1113, respectively. SD was linked to makers XB0207 and E0219 on the linkage group 2. The results would provide theoretical basis for gene fine mapping on seed related traits and gene clone.

Key words: melon, seed traits, genetic inheritance, QTL mapping

CLC Number:

S652

ZHANG Kexin, DAI Dongyang, WANG Haonan, YU Mingyue, SHENG Yunyan. Genetic and QTL analysis of seed traits in melon (Cucumis melon L.)[J]. , 2018, 30(9): 1496-1503.

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Genetic and QTL analysis of seed traits in melon (Cucumis melon L.)

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