›› 2019, Vol. 31 ›› Issue (5): 716-721.DOI: 10.3969/j.issn.1004-1524.2019.05.06

• Animal Science • Previous Articles     Next Articles

Proliferative characteristics of novel duck reovirus strain JDM10 in DF-1 cells

YUN Tao, HUA Jionggang, YE Weicheng, NI Zheng, CHEN Liu, ZHANG Cun*   

  1. Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agriculture Sciences, Hangzhou 310021, China
  • Received:2019-01-10 Online:2019-05-25 Published:2019-05-23

Abstract: To explore the proliferative charactistics of novel duck reovirus (NDRV) in chicken embryo fibroblast cells (DF-1). NDRV JDM10 strain was inoculated into DF-1 cells, and after continuous passage. The pathological effect (CPE) of virus on cells, half of tissue infection (TCID50), RT-PCR, indirect immunofluorescence (IFA) and western-blot immunology were studied. The results showed that NDRV JDM10 could effectivly proliferate in the DF-1 cells and produce typical CPE such as cell shedding, cell body enlargement and cell disruption. The σC gene (1 001 bp) was successfully amplified from the infected cells by RT-PCR. NDRV σC was expressed well in DF-1 cells and could specifically reacted with anti-NDRV σC monoclonal antibody. The one-step growth curve showed that NDRV JDM10 strains experienced three periods of incubation, rapid growth and stable phase after infection with DF-1 cells, and the virus titer reached peak at 72 h, and was TCID50 of 1×10-7.90·(0.1 mL)-1. These results established foundation for further study of the pathogenesis of NDRV and the development of cell vaccines.

Key words: novel duck reovirus (NDRV), DF-1 cells, proliferative characteristics

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