Comprehensive evaluation of salt tolerance of 22 alfalfa germplasms at germination stage

doi:10.3969/j.issn.1004-1524.2019.05.10

›› 2019, Vol. 31 ›› Issue (5): 746-755.DOI: 10.3969/j.issn.1004-1524.2019.05.10

• Horticultural Science • Previous Articles Next Articles

Comprehensive evaluation of salt tolerance of 22 alfalfa germplasms at germination stage

LI Mingyu¹, WANG Yan¹, LIANG Danni², YAO Yani³, LAN Jian^1,4,*

1. School of Agriculture, Ningxia University, Yinchuan 750021, China;
2. College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020;
3. Rural Practical Technology Training Center of Yuanzhou District, Guyuan 756000;
4. Modern Grassland Engineering Technology Research Center, Ningxia University, Yinchuan 750021, China

Received:2018-10-22 Online:2019-05-25 Published:2019-05-23

Abstract

Abstract: In order to screen salt-tolerance alfalfa germplasms and determine the appropriate concentration for salt-tolerance identification, the relative germination rate, relative germination energy, germination index, radicle dry weight and plumule dry weight were measured with conventional paper germination trial under different NaCl stress (0, 0.4%, 0.8%, 1.2%, 1.6%). Meanwhile, cluster analysis and comprehensive evaluation of salt-tolerance of 22 alfalfa germplasms were made. The results showed that relative germination rate, relative germination energy, germination index, radicle dry weight and plumule dry weight of alfalfa germplasms were all significantly lower than those of the control when the salt concentration increased. The semi-lethal salt concentration was 0.85% (variation coefficient was 26.38%), and 1.2% NaCl was the appropriate concentration to identify the salt tolerance of alfalfa. The salt-tolerant alfalfa germplasms were Herd King, Affinity, German Derby and Golden Empress, and the salt-sensitive alfalfa germplasms were Aohan, A/S Complex, Vernal and Ramble11. These results could provide references for the breeding of new salt-tolerant varieties.

Key words: alfalfa, salt tolerance, seed germination stage, cluster analysis

CLC Number:

LI Mingyu, WANG Yan, LIANG Danni, YAO Yani, LAN Jian. Comprehensive evaluation of salt tolerance of 22 alfalfa germplasms at germination stage[J]. , 2019, 31(5): 746-755.

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Comprehensive evaluation of salt tolerance of 22 alfalfa germplasms at germination stage

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