Identification of pathotype of Verticillium dahlida isolates on eggplant and their biological characteristics in Shaoxing City, Zhejiang Province

doi:10.3969/j.issn.1004-1524.2019.05.14

›› 2019, Vol. 31 ›› Issue (5): 784-789.DOI: 10.3969/j.issn.1004-1524.2019.05.14

• Plant Protection • Previous Articles Next Articles

Identification of pathotype of Verticillium dahlida isolates on eggplant and their biological characteristics in Shaoxing City, Zhejiang Province

LI Xuqing¹, ZHANG Jingze², ZHANG Ya¹, WU Genliang¹

1. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China;
2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

Received:2018-10-11 Online:2019-05-25 Published:2019-05-23

Abstract

Abstract: Based on the method of polymerase chain reaction (PCR) amplification using the specific primers of defoliation and nondefoliation pathotypes of Verticillium dahliae, the pathotype of the isolates causing the verticillium wilt disease of eggplant in Shaoxing City of Zhejiang Province were identified. And the biological characteristics of V. dahliae were also studied. Results of PCR amplification showed that the isolates (VD-sy1, VD-sy2, VD-sy3, VD-sy4 and VD-sy5) obtained all belonged to the defoliating pathotype. The results of biological characteristics showed that VD-sy1 was able to grow at pH between 4.0 to 9.0, and 25 ℃ under 24 h dark condition was beneficial to colony growth; sucrose and yeast extract were the best carbon and nitrogen source for mycelia with linear growth rates of 4.25 and 4.06 mm·d^-1, respectively, while galactose and ammonium chloride showed the least liner growth rates of 2.24 and 2.42 mm·d^-1, respectively.

Key words: Verticillium dahliae, pathotype, specific primer amplification, biological characteristics

CLC Number:

S432.4⁺4

LI Xuqing, ZHANG Jingze, ZHANG Ya, WU Genliang. Identification of pathotype of Verticillium dahlida isolates on eggplant and their biological characteristics in Shaoxing City, Zhejiang Province[J]. , 2019, 31(5): 784-789.

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Identification of pathotype of Verticillium dahlida isolates on eggplant and their biological characteristics in Shaoxing City, Zhejiang Province

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