›› 2019, Vol. 31 ›› Issue (5): 823-828.DOI: 10.3969/j.issn.1004-1524.2019.05.19

• Food Science • Previous Articles     Next Articles

Detection of Vibrio parahaemolyticus in seafood products by real-time quantitative PCR assay

CHEN Lin1,2, ZHOU Qingqing1, GU Qing1,*, LI Ping1   

  1. 1. Key Laboratory for Food Microbial Technology of Zhejiang Province, Zhejiang Gongshang University, Hangzhou 310018, China;
    2. Hangzhou Wahaha Group Co., Ltd., Hangzhou 310018, China
  • Received:2018-12-07 Online:2019-05-25 Published:2019-05-23

Abstract: Vibrio parahaemolyticus is a food-borne pathogen, which can contaminate food and cause serious and acute gastroenteritis to human beings. In this study, a real-time polymerase chain reaction (PCR) assay was developed for the quantitative detection of Vibrio parahaemolyticus in aquatic products. Randomly amplified polymorphic DNA (RAPD) method was introduced to characterize species-specific DNA fragment of V. parahaemolyticus, and specific primers were designed. The proposed method was used to detect V. parahaemolyticus in 20 aquatic products on sale. It was shown that 6 samples out of 20 were carrying V. parahaemolyticus. The minimum detection sensitivity was 50 fg DNA. Compared with the methods recommended in national standard, the proposed method was swifter and more convenient to detect V. parahaemolyticus in auqatic products.

Key words: Vibrio parahaemolyticus, randomly amplified polymorphic DNA, real-time polymerase chain reaction

CLC Number: