Association analysis between GnRH-1 gene polymorphism and egg quality in quail

doi:10.3969/j.issn.1004-1524.2023.03.09

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (3): 565-574.DOI: 10.3969/j.issn.1004-1524.2023.03.09

• Animal Science • Previous Articles Next Articles

Association analysis between GnRH-1 gene polymorphism and egg quality in quail

WANG Longwei¹(), BAI Junyan¹^,²^,^*(), JIA Xiaoping³, LEI Ying¹^,², CHEN Mengke¹, FAN Hongdeng¹, LU Xiaoning¹, HE Yuhan¹, ZENG Fanlin¹, ZHANG Rongkai¹

1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, Henan, China
2. Luoyang Key Laboratory of Animal Genetics and Breeding, Luoyang 471023, Henan, China
3. College of Agriculture/Tree Peony, Henan University of Science and Technology, Luoyang 471023, Henan, China

Received:2022-05-07 Online:2023-03-25 Published:2023-04-07

Abstract

Abstract:

To explore the correlation between GnRH-1 gene of gonadotropin releasing hormone (GnRH) gene subtype and quail egg quality, the single nucleotide polymorphism (SNP) mutation site of GnRH-1 gene in Chinese yellow quails, Beijing white quails and Korean quails was detected by PCR amplification and DNA sequencing, the polymorphism of GnRH-1 gene and its association with quality of quail eggs were analyzed in order to obtain the molecular markers related to the quality of quail eggs. The results showed that 14 SNP mutations of GnRH-1 gene were detected in Chinese yellow quails, Beijing white quails and Korean quails, which were C71T, C108T, C168T, C178T, A184G, C206T, A209C, C215T, A252G, A279T, C281T, C293G, C339T, C458T. Only two genotypes of A209C and C281T were detected in Chinese yellow quail and Beijing white quail, while other 12 SNP loci detected three genotypes in three quail varieties. Correlation analysis showed that there were significant (P<0.05) correlations between C71T, C215T, A209C and A279G loci of GnRH-1 gene and yolk height and yolk index, and between the A209C locus and the yolk width. There was a significant (P<0.05) correlation between A279G locus and eggshell thickness of quail. In conclusion, GnRH-1 gene could be used as a molecular marker or candidate gene for quail egg quality.

Key words: quail, GnRH-1gene, polymorphism, egg quality, yolk

CLC Number:

S839

WANG Longwei, BAI Junyan, JIA Xiaoping, LEI Ying, CHEN Mengke, FAN Hongdeng, LU Xiaoning, HE Yuhan, ZENG Fanlin, ZHANG Rongkai. Association analysis between GnRH-1 gene polymorphism and egg quality in quail[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 565-574.

Figures/Tables 5

References 23

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位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	CC	0.104	0.159	0.042	C215T	CC	0.354	0.659	0.250
	CT	0.417	0.318	0.313		CT	0.438	0.295	0.500
	TT	0.479	0.522	0.534		TT	0.208	0.046	0.250
C108T	CC	0.458	0.500	0.438	A252G	AA	0.104	0.205	0.125
	CT	0.417	0.319	0.417		AG	0.438	0.318	0.458
	TT	0.125	0.181	0.146		GG	0.458	0.477	0.417
C168T	CC	0.125	0.182	0.125	A279T	AA	0.085	0.100	0.109
	CT	0.417	0.341	0.479		AT	0.809	0.800	0.804
	TT	0.458	0.477	0.396		TT	0.106	0.100	0.087
C178T	CC	0.458	0.477	0.396	C281T	CC	0	0	0.063
	CT	0.417	0.341	0.438		CT	0.020	0.045	0.229
	TT	0.125	0.182	0.167		TT	0.980	0.955	0.708
A184G	AA	0.458	0.477	0.375	C293G	CC	0.104	0.159	0.042
	AG	0.417	0.341	0.458		CG	0.438	0.318	0.312
	GG	0.125	0.182	0.167		GG	0.458	0.523	0.646
C206T	CC	0.125	0.182	0.187	C339T	CC	0.833	0.795	0.688
	CT	0.438	0.364	0.542		CT	0.146	0.182	0.186
	TT	0.437	0.454	0.291		TT	0.021	0.021	0.125
A209C	AA	0	0	0.063	C458T	CC	0.563	0.341	0.604
	AC	0.020	0.045	0.313		CT	0.396	0.386	0.313
	CC	0.980	0.955	0.624		TT	0.041	0.273	0.083

位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	CC	0.104	0.159	0.042	C215T	CC	0.354	0.659	0.250
	CT	0.417	0.318	0.313		CT	0.438	0.295	0.500
	TT	0.479	0.522	0.534		TT	0.208	0.046	0.250
C108T	CC	0.458	0.500	0.438	A252G	AA	0.104	0.205	0.125
	CT	0.417	0.319	0.417		AG	0.438	0.318	0.458
	TT	0.125	0.181	0.146		GG	0.458	0.477	0.417
C168T	CC	0.125	0.182	0.125	A279T	AA	0.085	0.100	0.109
	CT	0.417	0.341	0.479		AT	0.809	0.800	0.804
	TT	0.458	0.477	0.396		TT	0.106	0.100	0.087
C178T	CC	0.458	0.477	0.396	C281T	CC	0	0	0.063
	CT	0.417	0.341	0.438		CT	0.020	0.045	0.229
	TT	0.125	0.182	0.167		TT	0.980	0.955	0.708
A184G	AA	0.458	0.477	0.375	C293G	CC	0.104	0.159	0.042
	AG	0.417	0.341	0.458		CG	0.438	0.318	0.312
	GG	0.125	0.182	0.167		GG	0.458	0.523	0.646
C206T	CC	0.125	0.182	0.187	C339T	CC	0.833	0.795	0.688
	CT	0.438	0.364	0.542		CT	0.146	0.182	0.186
	TT	0.437	0.454	0.291		TT	0.021	0.021	0.125
A209C	AA	0	0	0.063	C458T	CC	0.563	0.341	0.604
	AC	0.020	0.045	0.313		CT	0.396	0.386	0.313
	CC	0.980	0.955	0.624		TT	0.041	0.273	0.083

位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	C	0.315	0.318	0.199	C215T	C	0.573	0.807	0.500
	T	0.685	0.682	0.801		T	0.427	0.193	0.500
C108T	C	0.667	0.660	0.647	A252G	A	0.323	0.364	0.354
	T	0.333	0.340	0.353		G	0.677	0.636	0.646
C168T	C	0.333	0.353	0.365	A279T	A	0.489	0.500	0.511
	T	0.667	0.647	0.635		T	0.511	0.500	0.489
C178T	C	0.667	0.647	0.635	C281T	C	0.010	0.023	0.178
	T	0.333	0.353	0.365		T	0.990	0.977	0.922
A184G	A	0.667	0.647	0.604	C293G	C	0.323	0.318	0.198
	G	0.333	0.353	0.396		G	0.677	0.682	0.902
C206T	C	0.344	0.364	0.458	C339T	C	0.906	0.886	0.781
	T	0.656	0.636	0.542		T	0.094	0.114	0.219
A209C	A	0.010	0.023	0.220	C458T	C	0.761	0.534	0.761
	C	0.990	0.977	0.780		T	0.239	0.466	0.239

位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	C	0.315	0.318	0.199	C215T	C	0.573	0.807	0.500
	T	0.685	0.682	0.801		T	0.427	0.193	0.500
C108T	C	0.667	0.660	0.647	A252G	A	0.323	0.364	0.354
	T	0.333	0.340	0.353		G	0.677	0.636	0.646
C168T	C	0.333	0.353	0.365	A279T	A	0.489	0.500	0.511
	T	0.667	0.647	0.635		T	0.511	0.500	0.489
C178T	C	0.667	0.647	0.635	C281T	C	0.010	0.023	0.178
	T	0.333	0.353	0.365		T	0.990	0.977	0.922
A184G	A	0.667	0.647	0.604	C293G	C	0.323	0.318	0.198
	G	0.333	0.353	0.396		G	0.677	0.682	0.902
C206T	C	0.344	0.364	0.458	C339T	C	0.906	0.886	0.781
	T	0.656	0.636	0.542		T	0.094	0.114	0.219
A209C	A	0.010	0.023	0.220	C458T	C	0.761	0.534	0.761
	C	0.990	0.977	0.780		T	0.239	0.466	0.239

位点 Locus	基因型 Genotype	蛋质量 Egg weight/g	蛋形指数 Shape index	蛋黄高度 Yolk height/mm	蛋黄宽度 Yolk width/mm	蛋黄指数 Yolk index	蛋黄质量 Yolk weight/g	蛋壳厚度 Eggshell thickness/mm	蛋白高度 Protein height/mm
C71T	CC(n=14)	10.536 ±0.203 a	1.263 ±0.012 a	7.943 ±0.195 a	24.820 ±0.461 a	0.323 ±0.012 a	3.421 ±0.103 a	0.176 ±0.004 a	1.752 ±0.137 a
	CT(n=51)	10.402 ±0.130 a	1.278 ±0.007 a	7.380 ±0.129 b	25.276 ±0.256 a	0.295 ±0.007 b	3.424 ±0.064 a	0.168 ±0.003 a	1.659 ±0.065 a
	TT(n=78)	10.551 ±0.095 a	1.286 ±0.005 a	7.376 ±0.118 b	25.610 ±0.203 a	0.287 ±0.007 b	3.476 ±0.032 a	0.172 ±0.002 a	1.618 ±0.059 a
C108T	CC(n=66)	10.544 ±0.098 a	1.287 ±0.006 a	7.491 ±0.122 a	25.417 ±0.209 a	0.293 ±0.008 a	3.465 ±0.032 a	0.170 ±0.002 a	1.633 ±0.064 a
	CT(n=56)	10.461 ±0.132 a	1.278 ±0.007 a	7.339 ±0.127 a	25.363 ±0.258 a	0.292 ±0.007 a	3.434 ±0.060 a	0.170 ±0.003 a	1.681 ±0.063 a
	TT(n=21)	10.443 ±0.160 a	1.269 ±0.011 a	7.500 ±0.225 a	25.540 ±0.405 a	0.297 ±0.013 a	3.457 ±0.086 a	0.173 ±0.004 a	1.590 ±0.118 a
C168T	CC(n=20)	10.415 ±0.166 a	1.265 ±0.011 a	7.535 ±0.234 a	25.401 ±0.400 a	0.300 ±0.013 a	3.435 ±0.087 a	0.175 ±0.003 a	1.605 ±0.123 a
	CT(n=60)	10.443 ±0.126 a	1.278 ±0.006 a	7.308 ±0.121 a	25.444 ±0.246 a	0.290 ±0.006 a	3.442 ±0.057 a	0.170 ±0.002 a	1.659 ±0.061 a
	TT(n=63)	10.573 ±0.100 a	1.288 ±0.006 a	7.519 ±0.126 a	25.389 ±0.219 a	0.294 ±0.008 a	3.467 ±0.033 a	0.171 ±0.002 a	1.646 ±0.065 a
C178T	CC(n=63)	10.573 ±0.100 a	1.288 ±0.006 a	7.519 ±0.126 a	25.389 ±0.219 a	0.294 ±0.008 a	3.467 ±0.033 a	0.171 ±0.002 a	1.646 ±0.065 a
	CT(n=58)	10.419 ±0.129 a	1.277 ±0.007 a	7.326 ±0.125 a	25.348 ±0.245 a	0.292 ±0.007 a	3.421 ±0.057 a	0.170 ±0.002 a	1.678 ±0.061 a
	TT(n=22)	10.482 ±0.158 a	1.269 ±0.011 a	7.468 ±0.217 a	25.656 ±0.404 a	0.295 ±0.012 a	3.491 ±0.089 a	0.173 ±0.003 a	1.559 ±0.116 a
A184G	AA(n=62)	10.589 ±0.100 a	1.288 ±0.006 a	7.526 ±0.128 a	25.413 ±0.221 a	0.294 ±0.008 a	3.474 ±0.033 a	0.171 ±0.002 a	1.646 ±0.066 a
	AG(n=59)	10.405 ±0.127 a	1.277 ±0.006 a	7.322 ±0.122 a	25.323 ±0.242 a	0.292 ±0.006 a	3.414 ±0.056 a	0.170 ±0.002 a	1.677 ±0.060 a
	GG(n=22)	10.482 ±0.158 a	1.269 ±0.011 a	7.468 ±0.217 a	25.656 ±0.404 a	0.295 ±0.012 a	3.491 ±0.089 a	0.173 ±0.003 a	1.559 ±0.116 a
C206T	CC(n=22)	10.482 ±0.158 a	1.269 ±0.011 a	7.468 ±0.217 a	25.656 ±0.404 a	0.295 ±0.012 a	3.491 ±0.089 a	0.173 ±0.003 a	1.559 ±0.116 a
	CT(n=65)	10.403 ±0.121 a	1.277 ±0.006 a	7.280 ±0.116 a	25.385 ±0.238 a	0.290 ±0.006 a	3.426 ±0.052 a	0.170 ±0.002 a	1.669 ±0.056 a
	TT(n=56)	10.611 ±0.103 a	1.290 ±0.006 a	7.596 ±0.134 a	25.352 ±0.221 a	0.296 ±0.009 a	3.466 ±0.034 a	0.171 ±0.003 a	1.652 ±0.072 a
A209C	AA(n=3)	10.267 ±0.504 a	1.272 ±0.046 a	6.400 ±0.400 b	28.080 ±0.210 a	0.228 ±0.013 b	3.533 ±0.233 a	0.171 ±0.015 a	1.287 ±0.375 a
	AC(n=18)	10.717 ±0.234 a	1.290 ±0.009 a	6.700 ±0.245b	26.278 ±0.414b	0.257 ±0.012 ab	3.606 ±0.098 a	0.176 ±0.002 a	1.467 ±0.113 a
	CC(n=122)	10.470 ±0.077 a	1.280 ±0.004 a	7.566 ±0.083 a	25.220 ±0.158b	0.300 ±0.005 a	3.427 ±0.032 a	0.170 ±0.002 a	1.681 ±0.045 a
位点 Locus	基因型 Genotype	蛋质量 Egg weight/g	蛋形指数 Shape index	蛋黄高度 Yolk height/mm	蛋黄宽度 Yolk width/mm	蛋黄指数 Yolk index	蛋黄质量 Yolk weight/g	蛋壳厚度 Eggshell thickness/mm	蛋白高度 Protein height/mm
C215T	CC(n=60)	10.432 ±0.108 a	1.272 ±0.005 a	7.755 ±0.104 a	25.045 ±0.228 a	0.312 ±0.006 a	3.420 ±0.044 a	0.170 ±0.002 a	1.753 ±0.058 a
	CT(n=59)	10.553 ±0.122 a	1.293 ±0.007 a	7.239 ±0.119 b	25.660 ±0.231 a	0.284 ±0.006 b	3.493 ±0.053 a	0.173 ±0.003 a	1.577 ±0.069 a
	TT(n=24)	10.521 ±0.154 a	1.274 ±0.012 a	7.104 ±0.265 b	25.730 ±0.385 a	0.266 ±0.017 b	3.429 ±0.063 a	0.168 ±0.004 a	1.546 ±0.105 a
A252G	AA(n=20)	10.430 ±0.164 a	1.270 ±0.011 a	7.555 ±0.236 a	25.252 ±0.410 a	0.303 ±0.013 a	3.430 ±0.088 a	0.175 ±0.003 a	1.647 ±0.128 a
	AG(n=59)	10.459 ±0.126 a	1.279 ±0.007 a	7.319 ±0.122 a	25.494 ±0.245 a	0.290 ±0.006 a	3.444 ±0.058 a	0.169 ±0.002 a	1.648 ±0.061 a
	GG(n=64)	10.552 ±0.101 a	1.286 ±0.006 a	7.500 ±0.125 a	25.391 ±0.218 a	0.293 ±0.008 a	3.466 ±0.033 a	0.171 ±0.002 a	1.643 ±0.064 a
A279T	AA(n=14)	10.493 ±0.217 a	1.281 ±0.010 a	7.579 ±0.188 a	25.057 ±0.488 a	0.305 ±0.012 b	3.357 ±0.050 a	0.169 ±0.004 ab	1.773 ±0.127 a
	AT(n=115)	10.469 ±0.224 a	1.274 ±0.012 a	7.769 ±0.217 a	25.049 ±0.529 a	0.313 ±0.012 ab	3.425 ±0.112 a	0.164 ±0.005 b	1.801 ±0.110 a
	TT(n=14)	10.536 ±0.203 a	1.263 ±0.012 a	7.943 ±0.195 a	24.820 ±0.461 a	0.323 ±0.012 a	3.421 ±0.103 a	0.176 ±0.004 a	1.752 ±0.137 a
C281T	CC(n=3)	10.267 ±0.504 a	1.272 ±0.046 a	6.400 ±0.400 a	28.080 ±0.210 a	0.228 ±0.013 a	3.533 ±0.233 a	0.171 ±0.015 a	1.287 ±0.375 a
	CT(n=14)	10.557 ±0.289 a	1.285 ±0.012 a	6.714 ±0.295 a	26.146 ±0.456 a	0.259 ±0.014 a	3.557 ±0.107 a	0.176 ±0.003 a	1.520 ±0.136 a
	TT(n=126)	10.495 ±0.075 a	1.281 ±0.004 a	7.537 ±0.083 a	25.269 ±0.158 a	0.298 ±0.005 a	3.438 ±0.032 a	0.170 ±0.002 a	1.668 ±0.044 a
C293G	CC(n=14)	10.536 ±0.203 a	1.263 ±0.012 a	7.943 ±0.195 a	24.820 ±0.461 a	0.323 ±0.012 a	3.421 ±0.103 a	0.176 ±0.004 a	1.752 ±0.137 a
	CG(n=52)	10.404 ±0.128 a	1.277 ±0.007 a	7.388 ±0.126 a	25.329 ±0.256 a	0.294 ±0.007 a	3.433 ±0.064 a	0.168 ±0.003 a	1.650 ±0.064 a
	GG(n=77)	10.552 ±0.097 a	1.286 ±0.005 a	7.370 ±0.119 a	25.579 ±0.203 a	0.287 ±0.007 a	3.470 ±0.032 a	0.172 ±0.002 a	1.623 ±0.060 a
C339T	CC(n=110)	10.509 ±0.079 a	1.280 ±0.005 a	7.499 ±0.090 a	25.300 ±0.173 a	0.296 ±0.006 a	3.439 ±0.035 a	0.170 ±0.002 a	1.659 ±0.047 a
	CT(n=25)	10.324 ±0.187 a	1.281 ±0.010 a	7.220 ±0.219 a	25.446 ±0.324 a	0.286 ±0.011 a	3.448 ±0.066 a	0.172 ±0.002 a	1.591 ±0.102 a
	TT(n=8)	10.863 ±0.397 a	1.293 ±0.017 a	7.188 ±0.371 a	26.869 ±0.636 a	0.269 ±0.019 a	3.638 ±0.116 a	0.173 ±0.006 a	1.636 ±0.217 a
C458T	CC(n=72)	10.488 ±0.101 a	1.276 ±0.006 a	7.269 ±0.125 a	25.472 ±0.217 a	0.284 ±0.008 a	3.443 ±0.049 a	0.171 ±0.002 a	1.581 ±0.060 a
	CT(n=52)	10.462 ±0.124 a	1.290 ±0.006 a	7.504 ±0.124 a	25.458 ±0.252 a	0.297 ±0.007 a	3.481 ±0.045 a	0.170 ±0.003 a	1.669 ±0.068 a
	TT(n=19)	10.626 ±0.197 a	1.274 ±0.008 a	7.858 ±0.175 a	25.072 ±0.384 a	0.316 ±0.010 a	3.405 ±0.049 a	0.173 ±0.004 a	1.825 ±0.111 a

Association analysis between GnRH-1 gene polymorphism and egg quality in quail

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