Association analysis between GnRH-1 gene polymorphism and egg quality in quail

doi:10.3969/j.issn.1004-1524.2023.03.09

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (3): 565-574.DOI: 10.3969/j.issn.1004-1524.2023.03.09

• Animal Science • Previous Articles Next Articles

Association analysis between GnRH-1 gene polymorphism and egg quality in quail

WANG Longwei¹(), BAI Junyan¹^,²^,^*(), JIA Xiaoping³, LEI Ying¹^,², CHEN Mengke¹, FAN Hongdeng¹, LU Xiaoning¹, HE Yuhan¹, ZENG Fanlin¹, ZHANG Rongkai¹

1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, Henan, China
2. Luoyang Key Laboratory of Animal Genetics and Breeding, Luoyang 471023, Henan, China
3. College of Agriculture/Tree Peony, Henan University of Science and Technology, Luoyang 471023, Henan, China

Received:2022-05-07 Online:2023-03-25 Published:2023-04-07

Abstract

Abstract:

To explore the correlation between GnRH-1 gene of gonadotropin releasing hormone (GnRH) gene subtype and quail egg quality, the single nucleotide polymorphism (SNP) mutation site of GnRH-1 gene in Chinese yellow quails, Beijing white quails and Korean quails was detected by PCR amplification and DNA sequencing, the polymorphism of GnRH-1 gene and its association with quality of quail eggs were analyzed in order to obtain the molecular markers related to the quality of quail eggs. The results showed that 14 SNP mutations of GnRH-1 gene were detected in Chinese yellow quails, Beijing white quails and Korean quails, which were C71T, C108T, C168T, C178T, A184G, C206T, A209C, C215T, A252G, A279T, C281T, C293G, C339T, C458T. Only two genotypes of A209C and C281T were detected in Chinese yellow quail and Beijing white quail, while other 12 SNP loci detected three genotypes in three quail varieties. Correlation analysis showed that there were significant (P<0.05) correlations between C71T, C215T, A209C and A279G loci of GnRH-1 gene and yolk height and yolk index, and between the A209C locus and the yolk width. There was a significant (P<0.05) correlation between A279G locus and eggshell thickness of quail. In conclusion, GnRH-1 gene could be used as a molecular marker or candidate gene for quail egg quality.

Key words: quail, GnRH-1gene, polymorphism, egg quality, yolk

CLC Number:

S839

WANG Longwei, BAI Junyan, JIA Xiaoping, LEI Ying, CHEN Mengke, FAN Hongdeng, LU Xiaoning, HE Yuhan, ZENG Fanlin, ZHANG Rongkai. Association analysis between GnRH-1 gene polymorphism and egg quality in quail[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 565-574.

Figures/Tables 5

References 23

[1]	胡将智. 促性腺激素释放激素的研究进展[J]. 黑龙江科技信息, 2013(10): 236.
	HU J Z. Research progress of gonadotropin-releasing hormone[J]. Heilongjiang Science and Technology Information, 2013(10): 236. (in Chinese)
[2]	曹静, 陈耀星, 王子旭, 等. 促性腺激素释放激素的研究进展[J]. 中国畜牧兽医, 2007, 34(5): 48-51.
	CAO J, CHEN Y X, WANG Z X, et al. Progress on gonadotropin-releasing hormone[J]. China Animal Husbandry & Veterinary Medicine, 2007, 34(5): 48-51. (in Chinese with English abstract)
[3]	KING J A, MILLAR R P. Structure of chicken hypothalamic luteinizing hormone-releasing hormone Ⅱ: isolation and characterization[J]. Journal of Biological Chemistry, 1982, 257(18): 10729-10732. DOI URL
[4]	SHERWOOD N, EIDEN L, BROWNSTEIN M, et al. Characterization of a teleost gonadotropin-releasing hormone[J]. Proceedings of the National Academy of Sciences of the United States of America, 1983, 80(9): 2794-2798. PMID
[5]	SHAO Y T, TSENG Y C, CHANG C H, et al. GnRH mRNA levels in male three-spined sticklebacks, Gasterosteus aculeatus, under different reproductive conditions[J]. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2015, 180: 6-17. DOI URL
[6]	徐元青, 王建林, 邵宝平. 促性腺激素释放激素(GnRH)结构及调控的研究进展[J]. 畜牧与饲料科学, 2014, 35(12): 31-34.
	XU Y Q, WANG J L, SHAO B P. Research progress on structure and regulation of gonadotropin-releasing hormone(GnRH)[J]. Animal Husbandry and Feed Science, 2014, 35(12): 31-34. (in Chinese with English abstract)
[7]	SCHALLY A V, NAIR R M, REDDING T W, et al. Isolation of the luteinizing hormone and follicle-stimulating hormone-releasing hormone from porcine hypothalami[J]. The Journal of Biological Chemistry, 1971, 246(23): 7230-7236. DOI URL
[8]	张蕊, 常玲玲, 穆春宇, 等. 鸽GnIH和GnRH基因克隆及其在不同繁殖阶段下丘脑中的表达[J]. 中国畜牧兽医, 2018, 45(9): 2478-2485.
	ZHANG R, CHANG L L, MU C Y, et al. Cloning and expression of GnIH and GnRH genes in hypothalamus of pigeons at different breeding stages[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(9): 2478-2485. (in Chinese with English abstract)
[9]	张泽林. GnRH和LH在妊娠期母猪黄体的表达及作用研究[D]. 杨凌: 西北农林科技大学, 2019.
	ZHANG Z L. Expression and effects of GnRH and LH on corpus luteum of pregnant sows[D]. Yangling: Northwest A & F University, 2019. (in Chinese with English abstract)
[10]	HAMEED N, KHAN M I U R, AHMAD W, et al. Follicular dynamics, estrous response and pregnancy rate following GnRH and progesterone priming with or without eCG during non-breeding season in anestrous Beetal goats[J]. Small Ruminant Research, 2020, 182: 73-77. DOI URL
[11]	李志惠, 李德. 榕江小香羊GnRH基因多态性及其与繁殖性状关联性分析[J]. 湖北农业科学, 2016, 55(8): 2058-2061.
	LI Z H, LI D. Association analysis on polymorphism of GnRH gene and reproduction traits in Rongjiang small Xiang goat[J]. Hubei Agricultural Sciences, 2016, 55(8): 2058-2061. (in Chinese with English abstract)
[12]	黄种彬, 钟志新, 刘丽平, 等. 连城白鸭GnRH基因与产蛋性能的关联分析[J]. 中国家禽, 2011, 33(5): 31-33.
	HUANG Z B, ZHONG Z X, LIU L P, et al. Correlation analysis on single nucleotide polymorphism of GnRH gene and laying performance in Liancheng duck[J]. China Poultry, 2011, 33(5): 31-33. (in Chinese with English abstract)
[13]	杨涛. 鹅GnRH基因5'端调控区和外显子1 SNPs检测及与产蛋量的关系[D]. 合肥: 安徽农业大学, 2007.
	YANG T. SNPs in geese GnRH gene 5'-flank region and exon 1 and association with egg production[D]. Hefei: Anhui Agricultural University, 2007. (in Chinese with English abstract)
[14]	SOWER S A, FREAMAT M, KAVANAUGH S I. The origins of the vertebrate hypothalamic-pituitary-gonadal (HPG) and hypothalamic-pituitary-thyroid (HPT) endocrine systems: new insights from lampreys[J]. General and Comparative Endocrinology, 2009, 161(1): 20-29. DOI PMID
[15]	蒲跃进. 蛋用鹌鹑产蛋相关基因克隆、表达及其与性状的关联性研究[D]. 武汉: 华中农业大学, 2016.
	PU Y J. Molecular cloning and expression of genes related with laying quail reproduction and their associations with egg performances[D]. Wuhan: Huazhong Agricultural University, 2016. (in Chinese with English abstract)
[16]	NAKAYAMA Y, WONDISFORD F E, LASH R W, et al. Analysis of gonadotropin-releasing hormone gene structure in families with familial central precocious puberty and idiopathic hypogonadotropic hypogonadism[J]. The Journal of Clinical Endocrinology & Metabolism, 1990, 70(5): 1233-1238. DOI URL
[17]	MASON A J, PITTS S L, NIKOLICS K, et al. The hypogonadal mouse: reproductive functions restored by gene therapy[J]. Science, 1986, 234(4782): 1372-1378. PMID
[18]	颜泉梅, 陈秋菊, 崔易虹, 等. 西农莎能奶山羊和布尔山羊GnRH基因多态性与产羔数的相关分析[J]. 中国兽医学报, 2011, 31(11): 1667-1671.
	YAN Q M, CHEN Q J, CUI Y H, et al. Polymorphism of GnRH gene and its relationship with little size and growth weight of xinong shaaneng dairy goat and Boer goat[J]. Chinese Journal of Veterinary Science, 2011, 31(11): 1667-1671. (in Chinese with English abstract)
[19]	WU X, LI H F, YAN M J, et al. Associations of gonadotropin-releasing hormone receptor (GnRHR) and neuropeptide Y (NPY) genes’ polymorphisms with egg-laying traits in Wenchang chicken[J]. Agricultural Sciences in China, 2007, 6(4): 499-504. DOI URL
[20]	吴旭, 严美姣, 刘丽平, 等. 促性腺激素释放激素基因(GnRH)和生长激素基因(GH)对番鸭产蛋性能的遗传效应分析[J]. 农业生物技术学报, 2012, 20(3): 289-295.
	WU X, YAN M J, LIU L P, et al. Genetic effects of gonadotropin-releasing hormone(GnRH) and growth hormone (GH) genes on the egg performance in Muscovy duck(Cairina moschata)[J]. Journal of Agricultural Biotechnology, 2012, 20(3): 289-295. (in Chinese with English abstract)
[21]	彭亮. 番鸭GnRHR基因全长cDNA克隆、时空表达及对产蛋性能的关联研究[D]. 福州: 福建农林大学, 2013.
	PENG L. Full-length CDNA cloning of gonadotropin-releasing hormone receptor (GnRHR) gene, temporal and spatial expression and association studying on laying performance in Muscovy duck[D]. Fuzhou: Fujian Agriculture and Forestry University, 2013. (in Chinese with English abstract)
[22]	HU Y D, HUANG Q K, ZHU Q, et al. Identification and association of single-nucleotide polymorphisms in gonadotropin-inhibitory hormone (GnIH) gene with egg production traits in Erlang mountainous chickens[J]. Genetics and Molecular Research: GMR, 2015, 14(1): 294-303. DOI PMID
[23]	康乐. GnRH-Ⅰ基因多态性及其对鸡繁殖性能的影响[D]. 广州: 华南农业大学, 2007.
	KANG L. GnRH-Ⅰ gene polymorphism and its effect on chicken reproductive performance[D]. Guangzhou: South China Agricultural University, 2007. (in Chinese with English abstract)

位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	CC	0.104	0.159	0.042	C215T	CC	0.354	0.659	0.250
	CT	0.417	0.318	0.313		CT	0.438	0.295	0.500
	TT	0.479	0.522	0.534		TT	0.208	0.046	0.250
C108T	CC	0.458	0.500	0.438	A252G	AA	0.104	0.205	0.125
	CT	0.417	0.319	0.417		AG	0.438	0.318	0.458
	TT	0.125	0.181	0.146		GG	0.458	0.477	0.417
C168T	CC	0.125	0.182	0.125	A279T	AA	0.085	0.100	0.109
	CT	0.417	0.341	0.479		AT	0.809	0.800	0.804
	TT	0.458	0.477	0.396		TT	0.106	0.100	0.087
C178T	CC	0.458	0.477	0.396	C281T	CC	0	0	0.063
	CT	0.417	0.341	0.438		CT	0.020	0.045	0.229
	TT	0.125	0.182	0.167		TT	0.980	0.955	0.708
A184G	AA	0.458	0.477	0.375	C293G	CC	0.104	0.159	0.042
	AG	0.417	0.341	0.458		CG	0.438	0.318	0.312
	GG	0.125	0.182	0.167		GG	0.458	0.523	0.646
C206T	CC	0.125	0.182	0.187	C339T	CC	0.833	0.795	0.688
	CT	0.438	0.364	0.542		CT	0.146	0.182	0.186
	TT	0.437	0.454	0.291		TT	0.021	0.021	0.125
A209C	AA	0	0	0.063	C458T	CC	0.563	0.341	0.604
	AC	0.020	0.045	0.313		CT	0.396	0.386	0.313
	CC	0.980	0.955	0.624		TT	0.041	0.273	0.083

位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	CC	0.104	0.159	0.042	C215T	CC	0.354	0.659	0.250
	CT	0.417	0.318	0.313		CT	0.438	0.295	0.500
	TT	0.479	0.522	0.534		TT	0.208	0.046	0.250
C108T	CC	0.458	0.500	0.438	A252G	AA	0.104	0.205	0.125
	CT	0.417	0.319	0.417		AG	0.438	0.318	0.458
	TT	0.125	0.181	0.146		GG	0.458	0.477	0.417
C168T	CC	0.125	0.182	0.125	A279T	AA	0.085	0.100	0.109
	CT	0.417	0.341	0.479		AT	0.809	0.800	0.804
	TT	0.458	0.477	0.396		TT	0.106	0.100	0.087
C178T	CC	0.458	0.477	0.396	C281T	CC	0	0	0.063
	CT	0.417	0.341	0.438		CT	0.020	0.045	0.229
	TT	0.125	0.182	0.167		TT	0.980	0.955	0.708
A184G	AA	0.458	0.477	0.375	C293G	CC	0.104	0.159	0.042
	AG	0.417	0.341	0.458		CG	0.438	0.318	0.312
	GG	0.125	0.182	0.167		GG	0.458	0.523	0.646
C206T	CC	0.125	0.182	0.187	C339T	CC	0.833	0.795	0.688
	CT	0.438	0.364	0.542		CT	0.146	0.182	0.186
	TT	0.437	0.454	0.291		TT	0.021	0.021	0.125
A209C	AA	0	0	0.063	C458T	CC	0.563	0.341	0.604
	AC	0.020	0.045	0.313		CT	0.396	0.386	0.313
	CC	0.980	0.955	0.624		TT	0.041	0.273	0.083

位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	C	0.315	0.318	0.199	C215T	C	0.573	0.807	0.500
	T	0.685	0.682	0.801		T	0.427	0.193	0.500
C108T	C	0.667	0.660	0.647	A252G	A	0.323	0.364	0.354
	T	0.333	0.340	0.353		G	0.677	0.636	0.646
C168T	C	0.333	0.353	0.365	A279T	A	0.489	0.500	0.511
	T	0.667	0.647	0.635		T	0.511	0.500	0.489
C178T	C	0.667	0.647	0.635	C281T	C	0.010	0.023	0.178
	T	0.333	0.353	0.365		T	0.990	0.977	0.922
A184G	A	0.667	0.647	0.604	C293G	C	0.323	0.318	0.198
	G	0.333	0.353	0.396		G	0.677	0.682	0.902
C206T	C	0.344	0.364	0.458	C339T	C	0.906	0.886	0.781
	T	0.656	0.636	0.542		T	0.094	0.114	0.219
A209C	A	0.010	0.023	0.220	C458T	C	0.761	0.534	0.761
	C	0.990	0.977	0.780		T	0.239	0.466	0.239

位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	C	0.315	0.318	0.199	C215T	C	0.573	0.807	0.500
	T	0.685	0.682	0.801		T	0.427	0.193	0.500
C108T	C	0.667	0.660	0.647	A252G	A	0.323	0.364	0.354
	T	0.333	0.340	0.353		G	0.677	0.636	0.646
C168T	C	0.333	0.353	0.365	A279T	A	0.489	0.500	0.511
	T	0.667	0.647	0.635		T	0.511	0.500	0.489
C178T	C	0.667	0.647	0.635	C281T	C	0.010	0.023	0.178
	T	0.333	0.353	0.365		T	0.990	0.977	0.922
A184G	A	0.667	0.647	0.604	C293G	C	0.323	0.318	0.198
	G	0.333	0.353	0.396		G	0.677	0.682	0.902
C206T	C	0.344	0.364	0.458	C339T	C	0.906	0.886	0.781
	T	0.656	0.636	0.542		T	0.094	0.114	0.219
A209C	A	0.010	0.023	0.220	C458T	C	0.761	0.534	0.761
	C	0.990	0.977	0.780		T	0.239	0.466	0.239

位点 Locus	基因型 Genotype	蛋质量 Egg weight/g	蛋形指数 Shape index	蛋黄高度 Yolk height/mm	蛋黄宽度 Yolk width/mm	蛋黄指数 Yolk index	蛋黄质量 Yolk weight/g	蛋壳厚度 Eggshell thickness/mm	蛋白高度 Protein height/mm
C71T	CC(n=14)	10.536 ±0.203 a	1.263 ±0.012 a	7.943 ±0.195 a	24.820 ±0.461 a	0.323 ±0.012 a	3.421 ±0.103 a	0.176 ±0.004 a	1.752 ±0.137 a
	CT(n=51)	10.402 ±0.130 a	1.278 ±0.007 a	7.380 ±0.129 b	25.276 ±0.256 a	0.295 ±0.007 b	3.424 ±0.064 a	0.168 ±0.003 a	1.659 ±0.065 a
	TT(n=78)	10.551 ±0.095 a	1.286 ±0.005 a	7.376 ±0.118 b	25.610 ±0.203 a	0.287 ±0.007 b	3.476 ±0.032 a	0.172 ±0.002 a	1.618 ±0.059 a
C108T	CC(n=66)	10.544 ±0.098 a	1.287 ±0.006 a	7.491 ±0.122 a	25.417 ±0.209 a	0.293 ±0.008 a	3.465 ±0.032 a	0.170 ±0.002 a	1.633 ±0.064 a
	CT(n=56)	10.461 ±0.132 a	1.278 ±0.007 a	7.339 ±0.127 a	25.363 ±0.258 a	0.292 ±0.007 a	3.434 ±0.060 a	0.170 ±0.003 a	1.681 ±0.063 a
	TT(n=21)	10.443 ±0.160 a	1.269 ±0.011 a	7.500 ±0.225 a	25.540 ±0.405 a	0.297 ±0.013 a	3.457 ±0.086 a	0.173 ±0.004 a	1.590 ±0.118 a
C168T	CC(n=20)	10.415 ±0.166 a	1.265 ±0.011 a	7.535 ±0.234 a	25.401 ±0.400 a	0.300 ±0.013 a	3.435 ±0.087 a	0.175 ±0.003 a	1.605 ±0.123 a
	CT(n=60)	10.443 ±0.126 a	1.278 ±0.006 a	7.308 ±0.121 a	25.444 ±0.246 a	0.290 ±0.006 a	3.442 ±0.057 a	0.170 ±0.002 a	1.659 ±0.061 a
	TT(n=63)	10.573 ±0.100 a	1.288 ±0.006 a	7.519 ±0.126 a	25.389 ±0.219 a	0.294 ±0.008 a	3.467 ±0.033 a	0.171 ±0.002 a	1.646 ±0.065 a
C178T	CC(n=63)	10.573 ±0.100 a	1.288 ±0.006 a	7.519 ±0.126 a	25.389 ±0.219 a	0.294 ±0.008 a	3.467 ±0.033 a	0.171 ±0.002 a	1.646 ±0.065 a
	CT(n=58)	10.419 ±0.129 a	1.277 ±0.007 a	7.326 ±0.125 a	25.348 ±0.245 a	0.292 ±0.007 a	3.421 ±0.057 a	0.170 ±0.002 a	1.678 ±0.061 a
	TT(n=22)	10.482 ±0.158 a	1.269 ±0.011 a	7.468 ±0.217 a	25.656 ±0.404 a	0.295 ±0.012 a	3.491 ±0.089 a	0.173 ±0.003 a	1.559 ±0.116 a
A184G	AA(n=62)	10.589 ±0.100 a	1.288 ±0.006 a	7.526 ±0.128 a	25.413 ±0.221 a	0.294 ±0.008 a	3.474 ±0.033 a	0.171 ±0.002 a	1.646 ±0.066 a
	AG(n=59)	10.405 ±0.127 a	1.277 ±0.006 a	7.322 ±0.122 a	25.323 ±0.242 a	0.292 ±0.006 a	3.414 ±0.056 a	0.170 ±0.002 a	1.677 ±0.060 a
	GG(n=22)	10.482 ±0.158 a	1.269 ±0.011 a	7.468 ±0.217 a	25.656 ±0.404 a	0.295 ±0.012 a	3.491 ±0.089 a	0.173 ±0.003 a	1.559 ±0.116 a
C206T	CC(n=22)	10.482 ±0.158 a	1.269 ±0.011 a	7.468 ±0.217 a	25.656 ±0.404 a	0.295 ±0.012 a	3.491 ±0.089 a	0.173 ±0.003 a	1.559 ±0.116 a
	CT(n=65)	10.403 ±0.121 a	1.277 ±0.006 a	7.280 ±0.116 a	25.385 ±0.238 a	0.290 ±0.006 a	3.426 ±0.052 a	0.170 ±0.002 a	1.669 ±0.056 a
	TT(n=56)	10.611 ±0.103 a	1.290 ±0.006 a	7.596 ±0.134 a	25.352 ±0.221 a	0.296 ±0.009 a	3.466 ±0.034 a	0.171 ±0.003 a	1.652 ±0.072 a
A209C	AA(n=3)	10.267 ±0.504 a	1.272 ±0.046 a	6.400 ±0.400 b	28.080 ±0.210 a	0.228 ±0.013 b	3.533 ±0.233 a	0.171 ±0.015 a	1.287 ±0.375 a
	AC(n=18)	10.717 ±0.234 a	1.290 ±0.009 a	6.700 ±0.245b	26.278 ±0.414b	0.257 ±0.012 ab	3.606 ±0.098 a	0.176 ±0.002 a	1.467 ±0.113 a
	CC(n=122)	10.470 ±0.077 a	1.280 ±0.004 a	7.566 ±0.083 a	25.220 ±0.158b	0.300 ±0.005 a	3.427 ±0.032 a	0.170 ±0.002 a	1.681 ±0.045 a
位点 Locus	基因型 Genotype	蛋质量 Egg weight/g	蛋形指数 Shape index	蛋黄高度 Yolk height/mm	蛋黄宽度 Yolk width/mm	蛋黄指数 Yolk index	蛋黄质量 Yolk weight/g	蛋壳厚度 Eggshell thickness/mm	蛋白高度 Protein height/mm
C215T	CC(n=60)	10.432 ±0.108 a	1.272 ±0.005 a	7.755 ±0.104 a	25.045 ±0.228 a	0.312 ±0.006 a	3.420 ±0.044 a	0.170 ±0.002 a	1.753 ±0.058 a
	CT(n=59)	10.553 ±0.122 a	1.293 ±0.007 a	7.239 ±0.119 b	25.660 ±0.231 a	0.284 ±0.006 b	3.493 ±0.053 a	0.173 ±0.003 a	1.577 ±0.069 a
	TT(n=24)	10.521 ±0.154 a	1.274 ±0.012 a	7.104 ±0.265 b	25.730 ±0.385 a	0.266 ±0.017 b	3.429 ±0.063 a	0.168 ±0.004 a	1.546 ±0.105 a
A252G	AA(n=20)	10.430 ±0.164 a	1.270 ±0.011 a	7.555 ±0.236 a	25.252 ±0.410 a	0.303 ±0.013 a	3.430 ±0.088 a	0.175 ±0.003 a	1.647 ±0.128 a
	AG(n=59)	10.459 ±0.126 a	1.279 ±0.007 a	7.319 ±0.122 a	25.494 ±0.245 a	0.290 ±0.006 a	3.444 ±0.058 a	0.169 ±0.002 a	1.648 ±0.061 a
	GG(n=64)	10.552 ±0.101 a	1.286 ±0.006 a	7.500 ±0.125 a	25.391 ±0.218 a	0.293 ±0.008 a	3.466 ±0.033 a	0.171 ±0.002 a	1.643 ±0.064 a
A279T	AA(n=14)	10.493 ±0.217 a	1.281 ±0.010 a	7.579 ±0.188 a	25.057 ±0.488 a	0.305 ±0.012 b	3.357 ±0.050 a	0.169 ±0.004 ab	1.773 ±0.127 a
	AT(n=115)	10.469 ±0.224 a	1.274 ±0.012 a	7.769 ±0.217 a	25.049 ±0.529 a	0.313 ±0.012 ab	3.425 ±0.112 a	0.164 ±0.005 b	1.801 ±0.110 a
	TT(n=14)	10.536 ±0.203 a	1.263 ±0.012 a	7.943 ±0.195 a	24.820 ±0.461 a	0.323 ±0.012 a	3.421 ±0.103 a	0.176 ±0.004 a	1.752 ±0.137 a
C281T	CC(n=3)	10.267 ±0.504 a	1.272 ±0.046 a	6.400 ±0.400 a	28.080 ±0.210 a	0.228 ±0.013 a	3.533 ±0.233 a	0.171 ±0.015 a	1.287 ±0.375 a
	CT(n=14)	10.557 ±0.289 a	1.285 ±0.012 a	6.714 ±0.295 a	26.146 ±0.456 a	0.259 ±0.014 a	3.557 ±0.107 a	0.176 ±0.003 a	1.520 ±0.136 a
	TT(n=126)	10.495 ±0.075 a	1.281 ±0.004 a	7.537 ±0.083 a	25.269 ±0.158 a	0.298 ±0.005 a	3.438 ±0.032 a	0.170 ±0.002 a	1.668 ±0.044 a
C293G	CC(n=14)	10.536 ±0.203 a	1.263 ±0.012 a	7.943 ±0.195 a	24.820 ±0.461 a	0.323 ±0.012 a	3.421 ±0.103 a	0.176 ±0.004 a	1.752 ±0.137 a
	CG(n=52)	10.404 ±0.128 a	1.277 ±0.007 a	7.388 ±0.126 a	25.329 ±0.256 a	0.294 ±0.007 a	3.433 ±0.064 a	0.168 ±0.003 a	1.650 ±0.064 a
	GG(n=77)	10.552 ±0.097 a	1.286 ±0.005 a	7.370 ±0.119 a	25.579 ±0.203 a	0.287 ±0.007 a	3.470 ±0.032 a	0.172 ±0.002 a	1.623 ±0.060 a
C339T	CC(n=110)	10.509 ±0.079 a	1.280 ±0.005 a	7.499 ±0.090 a	25.300 ±0.173 a	0.296 ±0.006 a	3.439 ±0.035 a	0.170 ±0.002 a	1.659 ±0.047 a
	CT(n=25)	10.324 ±0.187 a	1.281 ±0.010 a	7.220 ±0.219 a	25.446 ±0.324 a	0.286 ±0.011 a	3.448 ±0.066 a	0.172 ±0.002 a	1.591 ±0.102 a
	TT(n=8)	10.863 ±0.397 a	1.293 ±0.017 a	7.188 ±0.371 a	26.869 ±0.636 a	0.269 ±0.019 a	3.638 ±0.116 a	0.173 ±0.006 a	1.636 ±0.217 a
C458T	CC(n=72)	10.488 ±0.101 a	1.276 ±0.006 a	7.269 ±0.125 a	25.472 ±0.217 a	0.284 ±0.008 a	3.443 ±0.049 a	0.171 ±0.002 a	1.581 ±0.060 a
	CT(n=52)	10.462 ±0.124 a	1.290 ±0.006 a	7.504 ±0.124 a	25.458 ±0.252 a	0.297 ±0.007 a	3.481 ±0.045 a	0.170 ±0.003 a	1.669 ±0.068 a
	TT(n=19)	10.626 ±0.197 a	1.274 ±0.008 a	7.858 ±0.175 a	25.072 ±0.384 a	0.316 ±0.010 a	3.405 ±0.049 a	0.173 ±0.004 a	1.825 ±0.111 a

Association analysis between GnRH-1 gene polymorphism and egg quality in quail

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