浙江农业学报
     Home  |  About Journal  |  Editorial Board  |  Instruction  |  Subscribe  |  Advertisement  |  Messages Board  |  Contact Us  |  中文
浙江农业学报
 
 
 
 
Forthcoming Articles
Current Issue
Archive
Advanced Search
Read Articles
Download Articles
Email Alert
2017 Vol. 29, No. 8
Published: 2017-08-20

Crop Science
Animal Science
Horticultural Science
Environmental Science
Agricultural Economy and Development
Biosystems Engineering
Reviews
 
 
       Crop Science
1221 Improving rice quality of ZH7954 by molecular marker-assisted selection
NIU Kaimeng, XU Junbo, ZHONG Liang, WANG Hua, ZHU Ying
DOI: 10.3969/j.issn.1004-1524.2017.08.01
Zhehui7954 (ZH7954) is an elite restorer line with high combining ability for three-line indica hybrid rice production. However, the amylose content of ZH7954 was high (25%), which resulted in poor eating and cooking quality. Improvement of rice quality of ZH7954 was hard to achieve by using traditional breeding strategies. In this study, a codominant molecular marker named PCR-AccI was developed based on the G/T SNP at the first intron splicing site of rice Wx gene which mainly determines the amylose biosynthesis in rice endosperm. Here, an elite parental line 93-11 with intermediate AC (17%) and T-type Wx(called Wxb) were used as donors. Two lines L2 and L9 were obtained with intermediate AC but similar phenotype to ZH7954 by backcross and molecular marker-assisted selection. Both of them had better eating and cooking quality than receptor line ZH7954, while the appearance quality, such as grain shape, grain weight and milled rice rate were almost identical to ZH7954. These results suggested that the quality of ZH7954 was successfully improved through combining the traditional breeding and marker assisted selection. Data from SNP-typing showed that less than 7% of genome from 93-11 was integrated to ZH7954 in both L2 and L9. The combining ability would be further tested and such intermediate lines might be the valuable materials to generate elite restorer line with high yield and good quality in the future.
2017 Vol. 29 (8): 1221-1227 [Abstract] ( 19 )   RICH HTML PDF (1613 KB)  ( 21 )
1228 Biological characteristics and utilization of new combinations in inter-subspecific crosses of rice
XU Weidong, LI Guan, CAI Jinyang, YANG Yaocheng
DOI: 10.3969/j.issn.1004-1524.2017.08.02
With utilization of the subspecies heterosis, the mid-japonica CMS line Chunjiang23A were crossed with six newly developed indica restorers and the biological characteristics such as agronomic traits and grain quality of the six combinations were investigated. The results showed that the duration from sowing to heading of the six hybrid combinations were significantly shorter than the control (CK) Liangyoupeijiu, and the plant height, the panicle length and the panicle number per plant were lower or less than CK, whereas the grain number per panicle and yield per plant were higher than CK. Identification of Cheng‘s indexes indicated that the six restorers belonged to the indicalinous. The results of molecular marker screening showed that five restorer lines had S5-n gene except the line Hm9175. The investigation of the grain quality showed that the combinations of Chunjiang23A/Hm9169 and Chunjiang23A/Hm9179 had better appearance qualities compared with the CK, and the amylose content of all the combinations showed no significant differences compared with the CK. The combinations developed from japonica cytoplasmic male sterility line and the indica-japonica intermediate type restorer lines showed strong hybrid subspecies heterosis and the grain yield increased obviously. However, the grain quality of the indica-japonica rice was still to be upgraded and improved.
2017 Vol. 29 (8): 1228-1233 [Abstract] ( 13 )   RICH HTML PDF (656 KB)  ( 13 )
1234 Effects of different irrigation amount on dry matter accumulation and transportation of spring maize under shallow subsurface drip irrigation
LI Yuanyuan, YANG Hengshan, ZHANG Ruifu, FAN Xiuyan, LI Jinqin, LIU Baolin, DELIGEER
DOI: 10.3969/j.issn.1004-1524.2017.08.03
In order to determine the suitable irrigation amount and provide reference for spring maize, effects of different irrigation amounts (0, 13, 26, 39, 52 mm every time under shallow subsurface drip irrigation and 80 mm every time under flood irrigation) on dry matter accumulation and transportation of spring maize were studied through field experiments using Zhengdan 958. The results showed that with the increase of irrigation amount, dry matter accumulation levels increased before silking, while increased first and then decreased after silking. From the composition of dry matter accumulation at immature stage, stems, leaves and stems+leaves+grains reached the maximum under the shallow subsurface drip irrigation 52 mm a time, and grains reached the maximum under the shallow subsurface drip irrigation 39 mm a time. Dry matter transportation amount, dry matter transportation efficiency and contribution rate of dry matter transportation to grain were increased first and then decreased with the the increase of irrigation amount, and reached the highest under the shallow subsurface drip irrigation 39 mm a time. The yield was significantly positively correlated with ear length, ear diameter, grain number per ear and 1 000-grain weight in the yield component factors. Compared with traditional flood irrigation 80 mm a time, yield of shallow subsurface drip irrigation 39 mm a time increased 6.03%, irrigation amount was saved 26.56% and economic benefit increased 6.78%. In the experimental area, the shallow subsurface drip irrigation 39 mm a time which could increase yield, save water and mulch was the suitable irrigation amount for high yield cultivation of spring maize.
2017 Vol. 29 (8): 1234-1242 [Abstract] ( 7 )   RICH HTML PDF (826 KB)  ( 7 )
       Animal Science
1243 Effects of concentrate level and dietary supplementation of recombinant antimicrobial peptides (AMPs) on growth performance and serum immunoglobulin, cytokines, complement, hormone levels in male Chuanzhong black goats
YANG Yanyi, DENG Junliang, CHEN Yun, GAO Shuang, LIU Qi, CHEN Chong, YAO Shuhua
DOI: 10.3969/j.issn.1004-1524.2017.08.04
This study is to evaluate the effects of dietary additive of antimicrobial peptides (AMPs) on growth performance, immunoglobulin, cytokines,complement and hormone levels of male Chuanzhong black goats. A total of 24 male goats were allotted to four groups on the basis of initial body weight, each group with 6 goats. Dietary treatments were Ⅰ-normal concentrate group (300 g concentrate), Ⅱ-normal concentrate and antimicrobial peptide group(300 g concentrate+3.0 g antimicrobial peptides), Ⅲ-high concentrate group (600 g concentrates), Ⅳ-high concentrate and antimicrobial peptide group (600 g concentrate+3.0g antimicrobial peptides). After a week of pre-feeding test, the 60 day test was conducted. On 0, 20th, 40th and 60th day, 4 goats were randomly selected from each group, whose jugular vein blood samples were collected to test immunoglobulin, cytokines, complement and hormone levels, and were weighed one by one. The results showed, compared with the control group, the recombinant antimicrobial peptides and high concentrate could significantly increase the levels of immunoglobulin IgA, IgG, IgM, cytokine IL-2, IL-6, IL-10, IL-12, IFN-γ, TNF-α, complement C3, C4, hormone ACTH, IGF-1, T3, T4 in serum, the body weights and average daily gains, which confirmed that the recombinant antimicrobial peptides and high concentrate could enhance the immune function and promote the growth of the goat.
2017 Vol. 29 (8): 1243-1252 [Abstract] ( 7 )   RICH HTML PDF (920 KB)  ( 14 )
1253 Analysis of T lymphocytes in peripheral blood of piglets in different growth stages
ZOU Lu, ZHANG Jingyan, ZHANG Kai, WANG Lei, ZHANG Kang, WANG Xurong, WANG Xuezhi, YU Sijiu, YANG Zhiqiang, LI Jianxi
DOI: 10.3969/j.issn.1004-1524.2017.08.05
To establish healthy piglets of peripheral blood lymphocyte subsets in clinical reference range, and analyze the level of immune system in different growth stages of piglets, the blood samples were collected on the 1st day, 7th day, 21st day and 35th day after weaning, respectively, and the mononuclear cells were isolated from the peripheral blood. Three color flow cytometry was used to detect the proportion of primary kinds of T lymphocyte subsets in peripheral blood mononuclear cells and in CD3+ T lymphocyte subsets, then the ratio of CD4+ /CD8+ was calculated. The proportion of CD3+ T lymphocyte subsets for peripheral blood mononuclear cells in male and female piglets were gradually increased from (38.00±10.94)% to (55.43±12.30)% and from (38.17±15.39)% to (51.57±10.60)% , respectively with the increase of age. On the 1st day and 7th day after weaning, the CD3+ T lymphocyte ratio had no significant difference between male and female piglets (P>0.05). On the 21st day after weaning, the CD3+ T lymphocyte ratio showed significant increase in male piglets(P<0.05). On the 35th day, the percentage of CD3+ T cells showed a significant increase (P<0.05). With the increase of age, the proportion of CD4+ T lymphocyte subsets in peripheral blood mononuclear cells of male and female piglets increased from (10.29±2.71)% to( 22.2±5.87)% and from (10.3±2.17)% to (23.4±6.84)%, respectively. The proportions of CD4+ T lymphocytes in male and female piglets were gradually increasing, and there was no significant difference between male and female piglets at different time points(P>0.05). The proportions of CD8+ T lymphocytes in peripheral blood mononuclear cells at the four time points were(23.80±6.67)%, (17.14±10.64)%, (25.46±12.32)%, (33.33±8.93)% in male piglets and (28.55±6.75)%, (23.62±11.51)%, (37.37±13.18)%, (38.31±15.66)% in female piglets. With the increase of age, the CD8+ T lymphocytes showed a trend of firstly decrease and then increase. The distribution of lymphocyte subsets in weaned piglets showed different changes with the increase of weaning time, and was influenced by gender. The combination of two different cell delineation methods could reflect more accurate changes of the number and proportion of lymphocyte subsets in piglets.
2017 Vol. 29 (8): 1253-1260 [Abstract] ( 6 )   RICH HTML PDF (1177 KB)  ( 9 )
1261 Effects of deltamethrin exposure on oxidative stress indexes and histological structure of hepatopancreas in Eriocheir sinensis
YANG Zongying, ZHANG Yiliu, HU Kun, YANG Xianle, LIU Lishuo, ZHANG Fengxiang, CAI Honggui
DOI: 10.3969/j.issn.1004-1524.2017.08.06
In order to explore the toxicological effects and mechanism of deltamethrin on Eriocheir sinensis, an acute semi-static toxic test was carried out, the LC50 values of 24, 48 and 96 h were 4.289 4, 3.481 8 and 1.319 5 μg·L-1, respectively, and the safe concentration was 0.658 9 μg·L-1. This study also revealed the modulation of antioxidant enzymes such as superoxide dismutase(SOD), catalase(CAT) and malondialdehyde (MDA) content, which was the main product of lipid peroxidation, at 6, 12, 24, 48 and 72 h in different deltamethrin concentration. 96 h-LC50/2(concentration I) group, 96 h-LC50/5(concentration II) group and 96 h-LC50/10(concentration III) group were test groups, and blank group and vehicle group were control groups. Histological structure of hepatopancreas was observed on 15th day in different deltamethrin concentrations. The results were as follows: (1) The oxidative stress indicators were varied in all groups exposed to deltamethrin, SOD and CAT activities showed similar variation of inhibition-induction-inhibition during the test, and the levels of MDA in test groups were all higher than that in blank group. The three indicators had no significant difference at different time in both vehicle group and blank group, and vehicle group also had no obvious difference from blank group. After 6 h exposed to deltamethrin, the CAT activities of all test groups were significantly lower than those of control group, and CAT activities of concentration II and III groups decreased by 37.96% and 38.92% compared with blank groups. MDA levels of test groups were obviously higher than that of control group. After 12 and 24 h, SOD and CAT activities were induced, SOD activities of three test groups were 72.13%, 87.93% and 83.20% higher than that of blank group, and the levels of MDA decreased at 24 h. After 48 h and 72 h, SOD and CAT activities were inhibited again, SOD activities of concentration I and III groups were 40.94% and 51.06% lower than that of blank group, CAT activities of concentration III group were 32.85% lower than that of blank group, and MDA levels of test groups gradually increased along with the increase of stress time of deltamethrin. (2) Histological structure of hepatopancreas was affected at different degrees in different deltamethrin concentration for 15 d. In these three test groups, the number of B cells of hepatopancreas was decreased, the basement membrane became thicker, some hepatic cells were swelling and cavity appeared, some vacuoles appeard in B cells. As deltamethrin concentration increased, the degree of deterioration of hepatopancreas aggravated, the number of vacuoles increased and some hepatic cells were crushing and many cell debris appeared in the lumen, many vacuoles existed between basement membrane and epithelial cells, part of the basement membranes were separated from epithelial cells and ruptured, nucleus were pyknotic. The results indicated that deltamethrin was high toxic pesticide to E.sinensis, with the deltamethrin concentration increasing and stress time prolonging, MDA levels increased, and the non-specific defense system and normal cell structure of E. sinensis were destroyed.
2017 Vol. 29 (8): 1261-1270 [Abstract] ( 14 )   RICH HTML PDF (1460 KB)  ( 7 )
1271 Genetic diversity and phylogenetic relationship among domestic and foreign pig breeds based on mtDNA COXⅠ gene
ZHOU Xiumin, YANG Yongjiang, BI Yingjie, REN Weihe, ZHANG Li
DOI: 10.3969/j.issn.1004-1524.2017.08.07
Based on the mitochondrial DNA COX Ⅰ (mtDNA COX Ⅰ) gene, the genetic diversity and phylogenetic relationships of 6 pig breeds (Large White pig, Landrace pig, Duroc pig, Zang pig, Gansu black pig and Bamei pig) were studied. The mtDNA COX Ⅰ gene sequences obtained from 6 pig breeds were sequenced and analyzed. Besides, we constructed phylogenetic tree by using MEGA 6.0 analysis software and neighbor joining method based on Kimurk two parameter models. The results showed that there were 21 mutations in sequences of COX Ⅰ (1 545 bp), including 8 group specific mutations. Landrace pig and Duroc pig were abundant in polymorphism (13 same mutation sites), and the ratios (R) of nucleotides’ conversion number (si) and transition number (sv) were 12 and 15, which proved sequence substitutions were far from saturation. While the polymorphisms of Zang pigs, Gansu black pigs and Bamei pigs were poor. The introduced pigs were used as sire, not dam, to improve the growth performance and lean meat percentage of the domestic pig breeds. The mtDNA COX Ⅰ gene can effectively distinguish the relationship between six pig breeds. This study can provide theoretical basis for the effective protection and rational utilization of pig breeds in our country.
2017 Vol. 29 (8): 1271-1280 [Abstract] ( 7 )   RICH HTML PDF (2375 KB)  ( 4 )
1281 Cloning and sequence analysis of growth hormone gene and its flanking region in topmouth culter (Culter alburnus Basilewsky)
LIU Shili, JIA Yongyi, JIANG Wenping, CHI Meili, CHENG Shun, ZHAO Jinliang, GU Zhimin, FU Jianjun
DOI: 10.3969/j.issn.1004-1524.2017.08.08
Using conserved primers and PCR, a gene encoding topmouth culter (Culter alburnus Basilewsky) growth hormone (CaGH) was amplified and sequenced. The gene spans 5 966 bp, including 2 282 bp of 5'- and 2 036 bp of 3'-flanking sequences and a 1.7-kb transcription unit comprised of five exons and five introns. (AAT)8 and (TTC)5T(TAA)8 microsatellite sequences were found in the 5'- and 3'-flanking regions, respectively. The upstream region contains TATA boxes, and binding sites of important transcription factors such as Pit-1, Pit-1a, CREB, AP1, GR, HNF-3, and HNF-3B. The five exons in Culter alburnus were 64 bp, 140 bp, 117 bp, 162 bp and 255 bp in length, respectively. The complete coding sequence was 603 bp and encodes a protein with a 22 amino acid signal peptide and a 178 amino acid mature peptide. Five conserved Cys residues (Cys71, Cys135, Cys173, Cys190, and Cys198) and two possible sites of N-glycosylation (residues 145 and 197) were detected in the GH polypeptide. The amino acid sequence of GH in Culter alburnus was identical to that in Megalobrama amblycephala, and only one amino acid residue differs from Ctenpharyngodon idellus. The phylogenetic relationships among the GH amino acid sequences in fish were in accord with traditional classification. The lengths of the four introns in Culter alburnus GH gene were 229 bp, 103 bp, 565 bp and 103 bp, respectively. The variation of the introns among species was greater than that of the exons, and the variation of the third intron is the highest. These results provided the molecular basis for study of function and transcriptional regulation of the GH gene in C.alburnus, as well as the temporal expression in different developmental stages and at various nutritional levels.
2017 Vol. 29 (8): 1281-1289 [Abstract] ( 4 )   RICH HTML PDF (3274 KB)  ( 5 )
1290 Tissue-specific expression analysis of ELOVL2 and ELOVL5 genes in Jingyuan chicken
MU Tong, ZHANG Juan, ZHAO Ping, GU Yaling, LIU Liyuan, YANG Yanjun, AN Kelong, WANG You
DOI: 10.3969/j.issn.1004-1524.2017.08.09
By using real-time quantitative PCR (qRT-PCR) expression of ELOVL2 and ELOVL5 in six different tissues (liver, muscle, heart, testis, ovary, abdominal fat) of Ningxia local varieties of Jingyuan chicken were determined, and the statistical analysis was carried out by SAS 8.2 software. The results showed that the expression of ELOVL2 and ELOVL5 genes were observed in six different tissues from Jingyuan chicken. The relative expression of ELOVL2 gene in liver was the highest, 71.52±0.41, significantly higher than that in other tissues (P<0.05), followed by the ovaries, testicles, abdominal fat, heart and muscle. There was a difference between the latter five tissues, but the difference was not significant (P>0.05). The relative expression of ELOVL5 gene in different tissues of Jingyuan chicken was as followed: liver>abdominal fat>ovary>testicle>heart>muscle. The highest expression level in liver was 110.94±0.02, which was significantly higher than that in other tissues (P<0.05), and the expression in abdominal fat was significantly higher than that in ovary, testis, heart and muscle (P<0.05). In addition, there was no significant difference in the expression in heart and muscle (P>0.05). The expression of ELOVL2 and ELOVL5 genes in different tissues was analyzed and compared. The results showed that the expression of two genes in abdominal fat was significantly different (P<0.05), and the expression of ELOVL5 gene in abdominal fat was higher. The research results showed that there were differences in the expression of ELOVL2 and ELOVL5 genes in six tissues from Jingyuan chicken, and liver tissue was the most obvious, which lays the foundation for the development of local technology platform to build local varieties of molecular breeding and genetic evaluation of germplasm resources.
2017 Vol. 29 (8): 1290-1296 [Abstract] ( 5 )   RICH HTML PDF (2239 KB)  ( 6 )
       Horticultural Science
1297 Influence of light on tissue culture of rose
LYU Yongping, CHEN Zhi, LI Kunfeng, WANG Yiting, MOU Haojie, CHEN Jianping
DOI: 10.3969/j.issn.1004-1524.2017.08.10
The effect of different color temperature white LEDs (Light-emitting diodes) and different combinations of red and blue LEDs on the tissue culture of rose were studied. Results showed that growth of plant materials cultured under the red and blue combination light qualities were significantly better than growth of those cultured under fluorescent and white LEDs in plant height uniform, while the growth of plant cultured under different color temperature white LEDs showed no significant difference from the growth of those cultured under fluorescent lamp. The results of photoperiod on tissue culture of rose indicated that when the LEDs illumination time was not shorter than 14 h·d-1, there was no significant difference in the plant material cultured under the white LEDs and the fluorescent material at 14 and 16 h·d-1. The results of rooting induction showed that plant cultured in different light circusmantance had no significant difference in rose rooting indutcion. All the results indicated that 5 000 K white LEDs could completely replace the fluorescent lamp as a rose tissue culture alternative light source, and 14 h·d-1 photoperiod was enough.
2017 Vol. 29 (8): 1297-1304 [Abstract] ( 7 )   RICH HTML PDF (2492 KB)  ( 32 )
1305 Effects of shrub invasion and grazing disturbance on population size and distribution of Pennisetum centrasiaticum
GU Junlong, WANG Xing, YANG Xinguo, ZHAO Wei, MIAO Fan, YANG Dongdong
DOI: 10.3969/j.issn.1004-1524.2017.08.11
To understand the effect of shrub invasion and grazing disturbance on the population size and distribution of Pennisetum centrasiaticum. Three sites comprising original grassland, transition zone of shrub-grassland and artificial shrub forest were selected under free grazed shrub forest in Yang zhaizi village within Yanchi country, Ningxia, northern China. The population features of Pennisetum centrasiaticum, soil nutrient, faeces counts and habitat conditions were surveyed at each site in grazed and fenced plots. We analyzed the relationship between population characteristic and variables environment factors. The results showed that: (1) From grassland to woodland, the number of Pennisetum centrasiaticum individuals declined, indicating alteration of its status within community from the dominant species to the associated species or rare species. (2) The coverage and abundance decreased in the area without shrub cover by the direct sheep feeding, this was an indirect process driving the aggregation of Pennisetum centrasiaticum individuals under the coverage of shrub. However, this distribution pattern changed under the fenced area in comparison to that in grazed area. (3) Potential factors in determining the population size and distribution pattern were density of shrub, intensity of grazing and protective effect of shrub. (4) For the desertified grassland dominated by Pennisetum centrasiaticum. The relatively stable diversity and production would be maintained under the moderate grazing condition. When the shrub invasion succeeded, the population of Pennisetum centrasiaticum could be protected by shrub through effect of conservation and resources enriching under a continuous grazing disturbance condition. However, due to the severe soil drought and occurring of soil crust associated with the development stage of shrub, the habitat would be harmful for the development of Pennisetum centrasiaticum population. Finally, fragmentation of population and declining in resistance of disturbance made the alteration of Pennisetum centrasiaticum status with community.
2017 Vol. 29 (8): 1305-1312 [Abstract] ( 6 )   RICH HTML PDF (1111 KB)  ( 10 )
1313 Callus induction and plant regeneration from stem explants of Campanumoea javanica B1.
QI Fuyou, FAN Weijun, HU Xiu, LIANG Hanzhi, WU Yongqing, XU Bingqiang
DOI: 10.3969/j.issn.1004-1524.2017.08.12
In order to estabilished an efficient plant regeneration system via callus from stem segments with bud of Campanumoea javanica B1. The aseptic stems were used as explants and then cultured on MS medium supplemented with different plant growth regulators (PGRs) to establish an effective protocol for callus induction, shoots induction and development, and roots induction. The results showed that two types of callus could be obtained under different treatments, of which type Ⅰ was yellow and compact while type Ⅱ was light yellow, fragile and mixed with a small number of white globular embryos. The type of callus could be regulated by cytokinin. Type I callus was induced on MS medium supplemented with 1.0 mg·L-1 2, 4-D+1.0 mg·L-1 6-BA + 0.5 mg·L-1 NAA, while type Ⅱ callus was induced on MS medium supplemented with 1.0 mg·L-1 2, 4-D. The compact callus could differentiated into adventitious buds on the medium supplementing cytokinin (6-BA, KT, TDZ). The adventitious buds further developed and proliferated after 30 d culture on MS medium containing 0.5 mg·L-1 6-BA+0.5 mg·L-1 GA3+0.1 mg·L-1 NAA. Excised shoots were treated with 300 mg·L-1 IBA for 15 min and then cultured on MS basal medium for 30 d, and the rooting rate reached 100%. When transplanted into a mixture of peat soil, vermiculite and perlite (volume ratios was 1∶1∶1), the survival rate of regenerated plantlets could reached 73.3% after 30 d. The experiment developed an effective protocol for C. javanica shoot organogenesis via callus, which might laid the foundation of researches on polyploidy, transgenic and cell mutation breeding.
2017 Vol. 29 (8): 1313-1320 [Abstract] ( 5 )   RICH HTML PDF (1443 KB)  ( 15 )
1321 Identification of volatile compounds in different vegetable soybean varieties and their differences
LIU Na, XU Shengchun, ZHANG Guwen, HU Qizan, FENG Zhijuan, Gong Yaming
DOI: 10.3969/j.issn.1004-1524.2017.08.13
The volatile compounds of five vegetable soybean varieties (Taiwan 75, Zhenong 8, Chunfengzao, Zhenong 3, Fucheng) were comparatively analyzed using solid-phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS). Results showed that 41 kinds of volatile components were detected, and 21, 23, 24, 26 and 18 kinds of volatile substances from Taiwan 75, Zhenong 8, Chunfengzao, Zhenong 3 and Fucheng, respectively. The ingredients for the characteristic volatile compounds of vegetable soybean were mainly determined to be alcohols and aldehydes. 1-octen-3-ol, cedrol, trans-farnesol, hexanal, nonanal and decanal were the main aroma in all different varieties. Principal component analysis (PCA) was analyzed for major aroma components in different vegetable soybean varieties. 1-hexanol and nonanal were higher in Taiwan 75 and Zhenong 3, which were the characteristic aroma in these two varieties. 1-octen-3-ol was the characteristic flavor in Zhenong 8, while ethyl iso-allocholate was the characteristic aroma in Fucheng which had specific taro flavor. The interaction of main aroma with different concentration leads to the specific flavor of each vegetable soybean. The research results would supply theoretical basis for breeding high quality varieties of vegetable soybean.
2017 Vol. 29 (8): 1321-1328 [Abstract] ( 6 )   RICH HTML PDF (1669 KB)  ( 5 )
       Environmental Science
1329 Study on spatial variability of soil available nutrients in tobacco planting soil in Yanyuan County
XIAO Yi, FENG Lang, WANG Peng, ZHANG Xin, LI Qiquan, WANG Yong, CHEN Yulan, LUO Lin, WANG Changquan
DOI: 10.3969/j.issn.1004-1524.2017.08.14
In order to reveal the spatial distribution of available nutrients in tobacco planting soils and the influence of previous crops, 273 soil samples were collected in tobacco-growing areas in Yanyuan County, and inverse distance weighting was adopted in combination with variance analysis. It was shown that the concentration of soil available N (AN), available P (AP) and available K (AK) ranged from 22.75 to 158.54, 1.25 to 68.90 and 34.37 to 362.59 mg·kg-1, respectively, and the variation coefficient of AN, AP and AK ranged from 29.17% to 77.04%. The spatial variability of AN, AP and AK was obvious. The concentrations of AN, AP and AK were mainly in the appropriate level, while the areas with lower AN, AP and AK were small, which were scattered plaques. The former crops had significant effects on soil AN and AP, yet no significant effect on AK in the study area. The average value of soil AN, AP and AK was the lowest when the previous crop was tobacco, which were 77.00, 13.15and 126.39 mg·kg-1 respectively. The average value of soil AP and AK was the highest when the previous crop was Vicia villosa, which was 27.06 and 170.41 mg·kg-1, respectively. The average value of soil AN was the highest when the previous crop was rice, which was 97.97 mg·kg-1.
2017 Vol. 29 (8): 1329-1335 [Abstract] ( 9 )   RICH HTML PDF (1234 KB)  ( 14 )
1336 Evaluation on agricultural water and soil resources security in Ningxia based on DPSIR model
ZHAO Ziyang, LI Wangcheng, ZHANG Yuzheng, WANG Xia
DOI: 10.3969/j.issn.1004-1524.2017.08.15
In the present study, an evaluation index system of agricultural water and soil resources security was constructed under the DPSIR model framework by structural equation modeling to quantitatively evaluate the water and soil resources security in Ningxia from 2004 to 2014, and to explore the optimal paths for water and soil resources security in Ningxia. It was shown that the evaluation indexes of driving force subsystem and state subsystem showed a steady upward development trend, and were increased from -1.172 and -1.067 in 2004 to 1.754 and 1.540 in 2014, respectively. The evaluation index of pressure subsystem continued to decrease. As the pressure subsystem was a negative index, it showed that the pressure of agriculture water and soil resources was increasing. Because the environment of Ningxia was sensitive to the global climate change, the evaluation index of impact subsystem didn’t show a clear trend. The evaluation index of response subsystem displayed steady and rapid growth except a single point anomaly in 2013. Overall, the agricultural water and soil resources security in Ningxia could be divided into 3 stages: (1) The security index increased from -0.434 in 2004 to 0.091 in 2008, despite that three years in the past five years were at a low level; (2) The security index decreased from 0.091 in 2008 to -0.344 in 2010, when the situation was not optimistic; (3) The security index increased from -0.344 in 2010 to 0.659 in 2014, when the intermediate level reached to 80%. The result of grey prediction showed that the agricultural water and soil resources security in Ningxia would be on a steady rise state in 2015-2010. The potential security index might rise from 0.523 to 1.204, and the security indexes would all be at high level. The security development of agricultural water and soil resources in Ningxia had some breakthrough, but it was still lying in the initial stage and had great potential.
2017 Vol. 29 (8): 1336-1346 [Abstract] ( 8 )   RICH HTML PDF (2342 KB)  ( 5 )
1347 Study on determination of exchangeable aluminum by inductively coupled plasma atomic emission spectrometry
JI Tianwei, SHEN Yue, CHEN Sili, FU Encheng
DOI: 10.3969/j.issn.1004-1524.2017.08.16
To analyze the soil exchangeable aluminum contents of soil samples, the inductively coupled plasma atomic emission spectrometry (ICP-AES) method was applied and compared with the neutralization titration method. Variance analysis showed that the measured value between the two methods was not statistically significant (P>0.05). The soil exchangeable aluminum was extracted using the soil-water ratios of 1∶50 and 1∶25, respectively, and then determined by both ICP-AES and neutralization titration method. Variance analysis showed that the aluminum content under 1∶50 extraction ratio was significantly (P<0.01) lower than that under 1∶50 extraction ratio. Two kinds of sample weight (5 g and 2 g) were also compared. It was shown that the soil sample weight could be cut down to 2 g. The optimum wavelength of analytical spectral line was 396.1 nm for soil exchangeable aluminum determination by ICP-AES, and the aluminum concentration for the standard curve was suggested as 0-50 mg·L-1. The detection limit, limited quantity and recovery rate of aluminum were 0.050 mg·L-1, 0.168 mg·L-1 and 98%, respectively.
2017 Vol. 29 (8): 1347-1352 [Abstract] ( 4 )   RICH HTML PDF (804 KB)  ( 4 )
1353 Study on relationship between heavy metals exposure and breast cancer
SUN Kewang, LIU Di, PAN Fengshan, WEI Yanyan, HOU Dandi, YANG Xiao’e
DOI: 10.3969/j.issn.1004-1524.2017.08.17
To explore the relationship between heavy metals exposure and breast cancer, 96 newly diagnosed cases of breast cancer (cancer group) and 30 matched normal cases (control group) were adopted as study materials, and the contents of heavy metals (Cd, Hg, As, Cr, Pb) in serum and breast tissues were determined by inductively coupled plasma mass spectrometry (ICP-MS). It was shown that Cd, Cr, As and Pb contents in serum and breast tissues in the cancer group were significantly (P<0.05) higher than those in the control group. Logistic regression analysis showed that Cd, Cr, As and Pb contents in the serum were associated with breast cancer risk. ORs of Cd, Cr, As and Pb were 12.364 (95%CI: 3.405-44.889), 132 (95%CI: 14.257-1 222.1), 4.941 (95%CI: 1.521-16.047) and 7.416 (95% CI: 0.326-9.980), respectively, indicating that exposure to environmental heavy metals such as Cd, Cr, As and Pb may be one of the risk factors for breast cancer.
2017 Vol. 29 (8): 1353-1357 [Abstract] ( 16 )   RICH HTML PDF (788 KB)  ( 4 )
1358 Analysis of vegetation buffer in river basin and its ecological evaluation
LIN Yi, JI Haowei
DOI: 10.3969/j.issn.1004-1524.2017.08.18
Based on the previous studies of existed basin buffer zone, the Landsat TM/ETM+satellite remote sensing image data of the Chaohu Lake Basin were selected as the research area. Firstly, the classification of land use was carried out on the image of the study area. On this basis, the terrain features were extracted from each catchment area, and then the buffer width was set up for each catchment area. Finally, the environmental sensitivity was evaluated. The research results could provide scientific basis for land use planning, ecological management, environmental protection and tourism resources development in the Chaohu Lake Basin.
2017 Vol. 29 (8): 1358-1364 [Abstract] ( 7 )   RICH HTML PDF (2174 KB)  ( 4 )
1365 Spatial distribution features of cultivated land quality based on spatial autocorrelation: A case study of Nanchang County, Jiangxi Province
ZHANG Han, ZHAO Xiaomin, KUANG Lihua, GUO Xi, LI Weifeng, OUYANG Zhencheng, HUANG Cong, WANG Xiaoyan, YE Yingcong
DOI: 10.3969/j.issn.1004-1524.2017.08.19
In order to understand and analyze the spatial characteristics of cultivated land quality, and to improve the quality of cultivated land construction and the new pattern of cultivated land protection, weighted average method, the index of concentration, fractal dimension and spatial autocorrelation analysis were adopted in the present study, and Nanchang County was selected as the study area to explore the differences of spatial characteristics of cultivated land quality from county and township-level scales by introducing the land natural grade index, use grade index and economical grade index as space variables and taking map spot of cultivated land as unit of spatial autocorrelation analysis. It was shown that the cultivated land quality index was high in southwest and low in northeast in Nanchang County. The Moran‘s I value decreased as land natural grade index>economical grade index>use grade index. Different types of cultivated land quality index had different spatial correlation under different spatial scales, and the influence spatial scales on land index decreased as land natural index>economical grade index>use grade index. The positive spatial autocorrelation of cultivated land which contained the High-High type and the Low-Low type emerged as the shape of cluster and had a powerful agglomeration in the form of group, while the negative spatial autocorrelation which included the High-Low type and Low-High type was fragmentary in distribution. Considering the spatial autocorrelation type of cultivated land quality in Nanchang County, the cultivated land was divided into 3 monitoring division, i.e. regular monitoring region, comprehensive monitoring region and key monitoring region. These findings would provide references for the monitoring of regional cultivated land quality, the determination of dominant limiting type order, protection of cultivated land and management of partition, quality improvement and optimization of the spatial allocation of land.
2017 Vol. 29 (8): 1365-1374 [Abstract] ( 8 )   RICH HTML PDF (3359 KB)  ( 7 )
       Biosystems Engineering
1375 Tomato seed varieties recognition based on principal component analysis and LVQ neural network
ZHAO Xueguan, WANG Xiu, LI Cuiling, GAO Yuanyuan, WANG Songlin, FENG Qingchun
DOI: 10.3969/j.issn.1004-1524.2017.08.20
In order to realize the real-time, accurate and no-damage mechanization identification of tomato seed varieties, according to the characteristics of tomato seeds and its image, the tomato varieties identification technology and algorithm were studied. This paper proposed a tomato seed varieties identification method, which is a kind of optimization by LVQ neural network based on principal components analysis, extracting the shape characteristics, texture feature and seven moment invariants of the tomato seeds. Four principal components as the input of artificial neural network were chosen through the principal components analysis. The identification test was conducted on five varieties of Heidi, Hongdi, Jiafen18, Jindi and Cupid. The number of competitive layer neurons and training trials were determined according to the test, which were 20 and 96. Under the condition, the average time of each seed identification was the shortest, and the recognition accuracy was the highest, which were 0.2 s and 90.5% respectively. The research showed that the method of identification and detection of tomato seed varieties based on machine vision is feasible.
2017 Vol. 29 (8): 1375-1383 [Abstract] ( 4 )   RICH HTML PDF (2062 KB)  ( 7 )
1384 Automatic extraction of plant diseases based on EM and K-means hybrid clustering
XIA Yongquan, WANG Bing, ZHI Jun, HUANG Haipeng, SUN Jingru
DOI: 10.3969/j.issn.1004-1524.2017.08.21
Aiming at the problem of how to extract the plant disease area accurately, a method based on EM and K-means hybrid clustering is proposed. The method can effectively extract the leaf target and have some screening effect on the more complicated background, which is superior to other classical methods in the case of obvious difference between the target and the background. Based on the chlorotic characteristics of the plant disease area, the K-means method was used to combine the Lab color space. Using the uniformity of the color distribution of the Lab color space, the A component was extracted as the reference component, and the disease area was extracted from the leaf target. The experimental results showed that the extraction of plant diseases based on EM and K-means hybrid clustering method is feasible.
2017 Vol. 29 (8): 1384-1391 [Abstract] ( 10 )   RICH HTML PDF (2461 KB)  ( 5 )
       Agricultural Economy and Development
1392 Discussion on strategy of agricultural sixth industrialization v2.0 based on production side enterprise brand
PAN Xiuyang, LIU Yue, KE Fuyan, TANG Yong
DOI: 10.3969/j.issn.1004-1524.2017.08.22
Under the framework of the sixth agricultural industrialization, when agricultural workers got involved in cross industry business, they faced many problems, such as lack of marketing capability and insufficient processing abilities, etc. Thus, it was difficult to enhance the market position of agriculture and related persons, nevertheless to promote the deep integration and development of tertiary industries related to agriculture. However, the agricultural enterprise brands based on the production side would be able to effectively remedy above shortcomings. In the present study, a new strategic conception of agricultural sixth industrialization v2.0 was proposed based on enterprise brands development dominated by the agricultural production side. It was suggested that professional farmers’ cooperatives should be the leading bodies in the process of forming new agricultural sixth industry business entities. The new entities should become the responsibility subject to implement brand innovation, promote industrial integration and increase the added value of agricultural products, and also to promote small-and medium-sized farmers to participate in, share and extend the added value of industries.
2017 Vol. 29 (8): 1392-1397 [Abstract] ( 4 )   RICH HTML PDF (921 KB)  ( 4 )
1398 Analysis on spatial characteristics and influence factors of farmers’ participation in land consolidation: A case of Hexi Corridor in Gansu Province
LIU Yang, CHEN Ying, ZHANG Yujiao, TIAN Feng
DOI: 10.3969/j.issn.1004-1524.2017.08.23
Based on the survey data of 15 counties’ rural land consolidation in Hexi Corridor, the spatial characteristics and influence factors of farmers’ participation in rural land consolidation were explored. The fuzzy comprehensive evaluation was used to measure the farmers’ participation, the exploratory spatial data analysis was used to determine the spatial characteristics and differences, and the spatial econometric model was applied to analyze the influence factors. The results showed that:(1) The farmers’ participation was generally low, and the spatial characteristics were obvious, which showed a significant positive spatial autocorrelation. (2) The “hot regions” of farmers’ participation were concentrated in Shiyang River and Shule River Basin, while Suzhou District, Gaotai County, Linze County were the “blind regions” in Black River Basin, showing a “dumbbell” structure. (3) The spatial error model showed that, the farmland blocks, the village manager‘s attitude towards rural land consolidation, farmers’ market expectations for farm products have a more significant effect on the spatial disparity of farmers’ participation. According to the actual situations, it is helpful to explore the spatial characteristics and influence factors of farmers’ participation for making different policies of the farmers’ participation for the government and land departments, and providing theoretical and method references for promoting the land consolidation performance and farmers’ participation.
2017 Vol. 29 (8): 1398-1408 [Abstract] ( 5 )   RICH HTML PDF (1528 KB)  ( 6 )
1409 Analysis into network transmissibility of Acta Agriculturae Zhejiangensis in 2013-2015: Taking CNKI data as an example
GAO Jun, YUAN Zuimin, ZHANG Yun, CHEN Huaping
DOI: 10.3969/j.issn.1004-1524.2017.08.24
In the present study, Acta Agriculturae Zhejiangensis was selected as an example, its network transmissibility in CNKI during 2013-2015 was analyzed from aspects of downloads and pageview, location of users, institutes of users, disciplines of the contents, and frequency distribution of downloads and pageview. It was shown that the network transmissiblity of Acta Agriculturae Zhejiangensis was improved, as the downloads and pageview steadily increased in 2013-2015. The location of uses covered all the main continents, including Asia, North America, Europe, Oceania, South America and Africa. In Asia, the uses covered 28 regions. In China, the users distributed all over 31 provinces. The main user institutes were agricultural universities. The disciplines of horticulture, plant protection, crop science, agricultural basic science, animal husbandry and animal medicine contributed the majority of downloads and pageview. The main frequency of download and pageview for a single article was around 10-50.
2017 Vol. 29 (8): 1409-1414 [Abstract] ( 5 )   RICH HTML PDF (1024 KB)  ( 6 )
       Reviews
1415 Research advance of molecular marker-assisted selection in tomato disease resistance breeding in China
CHEN Yuansong, ZHU Xiaowei, GONG Xiangyu, MEI Zhicheng, ZHU Biao
DOI: 10.3969/j.issn.1004-1524.2017.08.25
Disease resistance breeding is an important direction of tomato breeding. Molecular marker technology largely overcomes the limitations of phenotypic selection, greatly shortens the breeding cycle, and it has been widely used in tomato breeding. This paper reviewed the history and recent advances of molecular marker technology used in tomato disease resistance breeding in China. The characteristics of the main molecular markers and the main diseases in tomato disease resistance breeding were analyzed.
2017 Vol. 29 (8): 1415-1420 [Abstract] ( 10 )   RICH HTML PDF (2019 KB)  ( 10 )
Copyright © Editorial Board of
Supported by:Beijing Magtech