基于高通量测序技术的鱼类环境DNA研究中通用引物的筛选验证

张爱菊, 郝雅宾, 郭爱环, 刘金殿, 练青平, 周志明^*

Universal primer screening and verification for fish environmental DNA research based on high-throughout sequencing technology

ZHANG Aiju, HAO Yabin, GUO Aihuan, LIU Jindian, LIAN Qingping, ZHOU Zhiming^*

五对引物扩增水样eDNA的结果泳道1,GeneRuler 50 bp DNA ladder;泳道2—3,引物CY45M的扩增产物;泳道4—5,引物1634M的扩增产物;泳道6—7,引物16ACM的扩增产物;泳道8—9,引物COⅠM的扩增产物;泳道10—11,引物CYFM的扩增产物。