基于高通量测序技术的鱼类环境DNA研究中通用引物的筛选验证

张爱菊, 郝雅宾, 郭爱环, 刘金殿, 练青平, 周志明^*

Universal primer screening and verification for fish environmental DNA research based on high-throughout sequencing technology

ZHANG Aiju, HAO Yabin, GUO Aihuan, LIU Jindian, LIAN Qingping, ZHOU Zhiming^*

引物1634M扩增水样eDNA样品的结果泳道1,GeneRuler 50 bp DNA ladder;泳道2—10,引物1634M依次以1至9号水样eDNA为模板的扩增产物;泳道11,阴性对照。