›› 2013, Vol. 25 ›› Issue (1): 0-186.

• 论文 •    

新疆高寒牧区产细菌素低温乳酸菌的筛选

王俊钢1,刘成江1,郭安民1,李宇辉1,韩冬印2,李开雄3   

  1. 1新疆农垦科学院 农产品加工研究所, 新疆 石河子 832000;2新疆雨润食品有限责任公司, 新疆 石河子 832000;3石河子大学 食品学院, 新疆 石河子 832000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-01-25 发布日期:2013-01-25

Screening of bacteriocin\|producing low\|temperature lactic acid bacteria in high,cold pasture areas in Xinjiang

WANG Jungang;LIU Chengjiang;GUO Anmin;LI Yyuhui;HAN Dongyin;LI Kaixiong   

  1. 1Institute of Agro\|products Processing Science and Technology, Xinjiang Academy of Agricultural and Reclamation Science; Shihezi 832000, China;2Yurun Food Limited Company of Xinjiang; Shihezi 832000, China; 3 Food College of Shihezi University, Shihezi 832000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-01-25 Published:2013-01-25

摘要: 从新疆高寒牧区牧民自制乳制品分离得到45株乳酸菌。采用排除有机酸和过氧化氢中和试验对分离的乳酸菌进行处理,结果表明三株乳酸菌有抑菌特性。用胰蛋白酶对发酵液进行处理后,其抑菌活性大大降低,说明代谢产物是具有蛋白属性的抑菌物质,判断为细菌素。鉴定结果初步认为,这三株乳酸菌分别属于植物乳杆菌、戊糖乳杆菌和乳酸乳球菌。

关键词: 新疆, 低温乳酸菌, 细菌素

Abstract: Forty\|five lactic acid bacteria were screened from the samples collected from the high, cold pasture areas in Xinjiang, and three of them with antimicrobial properties were screened out through the test. After exclusion of organic acids and catalase, these three lactic acid bacteria still had a certain bacteriostasis performance. Meanwhile, the antibacterial activity was greatly reduced after treated with trypsin, which indicated that the metabolite was bacteriocins, the protein with antibacterial property. These three lactic acid bacteria were identified to be: Lactobacillus plantarum, Lactobacillus pentosus and Lactococcus lactis,respectively.

Key words: Xinjiang, low\|temperature lactic acid bacteria, bacteriocin