烟草根特异性启动子植物表达载体的构建及其对番茄的转化

›› 2009, Vol. 21 ›› Issue (1): 0-5.

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烟草根特异性启动子植物表达载体的构建及其对番茄的转化

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收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-01-25 发布日期:2009-01-25

Construction of expression vector driven by tobacco root-specific promoter and Agrobacterium, mediated transformation of tomato

PENG Juan;LI Zhi-miao;YANG Yue-jian;YE Qing-jing;WU Cai-jun;ZHANG Xiao-ming

1 Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China;2 College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, China;3 Institute of Crops and Utilization of Nuclear Technology, Hangzhou, 310021, China

Received:1900-01-01 Revised:1900-01-01 Online:2009-01-25 Published:2009-01-25

摘要/Abstract

摘要： 利用PCR技术从烟草中克隆获得了893bp的根特异性表达基因TobRB7的启动子，命名为NT1。以pBI121为原始植物表达载体，用NT1启动子和番茄广谱抗病基因Pro分别取代CaMV 35S启动子和GUS基因，获得了烟草根特异启动子驱动抗病基因的表达载体，命名为ρBI-NTI：：Pto。采用冻融法将pBI—NT1：：Pto导入根癌农杆菌EHA105中，以番茄子叶为外植体进行了侵染转化。PCR检测NPT Ⅱ基因的结果表明已获得转基因番茄植株。

关键词: >番茄 >根特异启动子 >表达载体 >转基因

Abstract: The 893bp fragment of root-specific promoter of tobacco TobRB7 gene, namely NT1, was cloned by PCR. The expression vector pBI-NT1 ：： Pro, driven by tobacco root-specific promoter NT1, was constructed from pBI121 by substituting the CaMV 35S promoter and GUTS gene with NT1 and Pro, a tomato broad-spectrum R gene, respectively. Through freeze and thaw method, the pBI-NT1 ：： Pro vector was introduced into Agrobacterium tumefaciens strain EHA105, and the explants of tomato cotyledons were then transformed. PCR test result of NPT H gene indicated that transgenic tomato lines were obtained.

Key words: tomato, root-specific promoter, expression vector, transgene

<A href=. 烟草根特异性启动子植物表达载体的构建及其对番茄的转化[J]. , 2009, 21(1): 0-5.

PENG Juan;LI Zhi-miao;YANG Yue-jian;YE Qing-jing;WU Cai-jun;ZHANG Xiao-ming. Construction of expression vector driven by tobacco root-specific promoter and Agrobacterium, mediated transformation of tomato[J]. , 2009, 21(1): 0-5.

烟草根特异性启动子植物表达载体的构建及其对番茄的转化

Construction of expression vector driven by tobacco root-specific promoter and Agrobacterium, mediated transformation of tomato

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