Abstract: The plum cultivar Baicui Jimali 1 selected from Sanhua plum， was used to optimize the factors of SRAP-PCR step by step. The results indicated that the optimal SRAP-PCR system for Sanhua plum in a 25 μL PCR reaction consisted of 15 ng DNA template， 3.0 mmol·L-1 Mg2+， 0.25 μmol·L-1 primer， 0.3 mmol·L-1 dNTP， 1.0 U Taq DNA polymerase， 2.5 μL 10×Buffer and ddH2O. Application of this optimum reaction system in eighteen accessions of plum by 6％denaturing polyacrylamide gel produced clear polymorphic patterns. It is demonstrated that the optimal SRAP-PCR system can be used for molecular research of Sanhua plum.

Key words: Sanhua plum, SRAP\|PCR reaction system, optimization