水稻黑条矮缩病毒P91在烟草表皮细胞中自我互作并形成包涵体

›› 2014, Vol. 26 ›› Issue (2): 0-365370.

• 植物保护 •

水稻黑条矮缩病毒P91在烟草表皮细胞中自我互作并形成包涵体

谭子龙1，2，孙丽英2，*，陈剑平2，*

1浙江师范大学化学与生命科学学院，浙江金华321004；2浙江省农业科学院病毒学与生物技术研究所/浙江省植物有害生物防控国家重点实验室培育基地/农业部植物保护与生物技术重点开放实验室/浙江省植物病毒重点开放实验室，浙江杭州310021

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-03-25 发布日期:2014-07-09

Rice black streaked dwarf virus P91，selfinteracts and forms inclusion bodies in epidermal cells of Nicotiana benthamiana plants

TAN Zilong;SUN Liying;*;CHEN Jianping;*

1College of Chemistry and Life Science， Zhejiang Normal University， Jinhua 321004， China；2State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control/Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection/Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences， Hangzhou 310021， China

Received:1900-01-01 Revised:1900-01-01 Online:2014-03-25 Published:2014-07-09

摘要/Abstract

摘要： 水稻黑条矮缩病毒(Rice blackstreaked dwarf virus，RBSDV)是斐济病毒属的成员，由昆虫介体灰飞虱传播，能够在植物宿主和昆虫介体中复制。同其他斐济病毒成员一样，RBSDV的复制与装配是在细胞质病毒包涵体结构即毒质结构里进行的。RBSDV有10条基因组dsRNAs(S1—S10)，其第9条片段(S9)的第一个阅读框(ORF)编码的蛋白P91是形成毒质结构的框架蛋白。将P91与绿色荧光蛋白(GFP)融合后，在本氏烟草的表皮细胞中单独表达，通过激光共聚焦扫描显微镜观察发现P91可以形成包涵体结构；利用双分子荧光互补(BiFC)实验证明了P91具有自我互作的能力，并形成包涵体结构。

关键词: 水稻黑条矮缩病毒, 包涵体, 自我互作

Abstract: Rice blackstreaked dwarf virus (RBSDV)， a member of the genus Fijivirus， is transmitted in nature by the small brown planthopper (Laodelphax striatellus) and can replicate in cells of both host plants and insect vector. RBSDV， like other members of the family Reovirus， is thought to replicate and assemble within cytoplasmic viral inclusion bodies， commonly called viroplasms. It has genomic segments of 10 dsRNAs (S1-S10) and P91 encoded by the first open reading frame in the ninth segment (S9) of the genomic RNAs is an essential protein for the formation of viroplasms. Fused with green fluorescent protein (GFP)， P91 was able to form inclusion bodies when expressed alone in Nicotiana benthamiana epidermal cell. Bimolecular fluorescence complementation (BiFC) assay suggested P91 had the ability of selfinteraction and formed inclusion bodies．

Key words: Rice black streaked dwarf virus, inclusion bodies, selfinteraction

谭子龙;孙丽英;*;陈剑平;* . 水稻黑条矮缩病毒P91在烟草表皮细胞中自我互作并形成包涵体[J]. , 2014, 26(2): 0-365370.

TAN Zilong;SUN Liying;*;CHEN Jianping;*. Rice black streaked dwarf virus P91，selfinteracts and forms inclusion bodies in epidermal cells of Nicotiana benthamiana plants[J]. , 2014, 26(2): 0-365370.

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水稻黑条矮缩病毒P91在烟草表皮细胞中自我互作并形成包涵体

Rice black streaked dwarf virus P91，selfinteracts and forms inclusion bodies in epidermal cells of Nicotiana benthamiana plants

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