药用植物淫羊藿与富血小板血浆协同对软骨细胞的增殖作用

›› 2014, Vol. 26 ›› Issue (3): 0-680684.

• 园艺科学 •

药用植物淫羊藿与富血小板血浆协同对软骨细胞的增殖作用

张蕾1，2，程瑜3，金王东3，王飘逸1，单乐天3，*

1浙江科技学院生物与化学工程学院，浙江杭州 310023；2浙江省农产品化学与生物加工技术重点实验室，浙江杭州 310023；3浙江中医药大学骨伤研究所，浙江杭州 310053

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-05-25 发布日期:2014-05-25

Study of medicinal plant Epimedium brevicornum Maxim and platelet effects on chondrocyte proliferation

ZHANG Lei;CHENG Yu;JIN Wangdong;WANG Piaoyi;SHAN Letian;*

1Zhejiang University of Science & Technology，Hangzhou 310002，China; 2Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Agricultural Products，Hangzhou 310023，China;3Zhejiang Chinese Medical University， Hangzhou 310053， China

Received:1900-01-01 Revised:1900-01-01 Online:2014-05-25 Published:2014-05-25

摘要/Abstract

摘要： 观察淫羊藿和富血小板血浆(PRP)对SD大鼠软骨细胞增殖作用的影响，探讨两者对软骨细胞的协同作用。取3周龄SD大鼠的膝关节软骨建立软骨细胞体外培养体系，分别采用MTT法、流式细胞术对细胞增殖作用进行检测。结果表明: (1) PRP对软骨细胞增殖有显著的促进作用(P<001)；(2)淫羊藿对软骨细胞有一定的增殖作用，但不显著；(3)淫羊藿联合PRP对软骨细胞的增殖作用显著下降，效果低于淫羊藿或PRP组(P<001)。淫羊藿联合PRP对软骨细胞有显著抑制作用(P<001)，推断淫羊藿某些成分抑制了PRP中生长因子的活性，从而减弱了PRP对软骨细胞的作用，该推断有待进一步研究确认。

关键词: 骨性关节炎, 淫羊藿, 富血小板血浆, 软骨细胞, 细胞增值

Abstract: To observe the proliferative effects of Epimedium brevicornum Maxim and plateletrich plasma (PRP)， respectively， on SD rat chondrocyte， and to explore their synergic effect between each other, in vitro chondrocyte culter system was established using 3weekold SD rat knee cartilage. MTT and flow cytometry assays were adopted to test the chondrocyte prolifereation. Results: （1） PRP has significant proliferative effect on chondrocyte (P<001); （2） Epimedium brevicornum Maxim has a bit proliferative effect on chondrocyte; （3） Epimedium brevicornum Maxim combined with PRP has inhibitory effect on chondrocyte (P<001). Epimedium brevicornum Maxim combined PRP has inhibitory effect on chondrocyte (P<001)， deducing that some components in Epimedium brevicornum Maxim inhibited PRP growth factors and then decrease PRP activity. The conclusion needs further investigation．

Key words: osteoarthritis, Epimedium brevicornum Maxim, plateletrich plasma, chondrocytes, proliferation

张蕾;程瑜;金王东;王飘逸;单乐天;*. 药用植物淫羊藿与富血小板血浆协同对软骨细胞的增殖作用[J]. , 2014, 26(3): 0-680684.

ZHANG Lei;CHENG Yu;JIN Wangdong;WANG Piaoyi;SHAN Letian;*. Study of medicinal plant Epimedium brevicornum Maxim and platelet effects on chondrocyte proliferation[J]. , 2014, 26(3): 0-680684.

药用植物淫羊藿与富血小板血浆协同对软骨细胞的增殖作用

Study of medicinal plant Epimedium brevicornum Maxim and platelet effects on chondrocyte proliferation

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