响应面分析法优化超声提取连翘花黄色素工艺研究

浙江农业学报

响应面分析法优化超声提取连翘花黄色素工艺研究

运城学院生命科学系, 山西运城 044000

出版日期:2014-07-25 发布日期:2014-08-06

Optimization on ultrasonic extraction of total yellow pigment from Forsythia suspensa Vahl. based on response surface methodology#br#

Department of Life Science， Yuncheng University， Yuncheng 044000， China

Online:2014-07-25 Published:2014-08-06

摘要/Abstract

摘要： 以黄色素吸光度为衡量指标，在单因素试验基础上，运用BoxBehnken 中心组合采用四因子三水平试验模型，建立回归方程，以响应面分析法(RSM)对连翘花黄色素的提取工艺进行优化。结果表明，以料液比1∶28(V/m)，乙醇浓度96%，超声时间31 min，超声温度72℃为提取条件，实际平均色素吸光度为0735　5，理论值为0755　7，二者相差较小。

关键词: 连翘, 黄色素, 响应面分析法(RSM), 超声提取

Abstract: The absorbance of yellow pigment was used as the measurement indicator. The regression equation was established by using four factorsthree levels experimental model of BoxBehnken central composite on the basis of single factor test. The extraction method of yellow pigment from Forsythia suspensa Vahl. was optimized by response surface methodology. The results showed that optimal extraction conditions were as follows: solidliquid ratio of 1∶28， alcohol concentration of 96%， ultrasonic time of 31 min， ultrasonic temperature of 72℃. The practical value was 07355， which was close to the model prediction extraction absorbance of 07557.

Key words: Forsythia suspensa Vahl., yellow pigment, response surface methodology (RSM), ultrasonic extraction

王玲丽，滕红梅*，弓苗苗. 响应面分析法优化超声提取连翘花黄色素工艺研究[J]. 浙江农业学报.

WANG Lingli， TENG Hongmei*， GONG Miaomiao. Optimization on ultrasonic extraction of total yellow pigment from Forsythia suspensa Vahl. based on response surface methodology#br#[J]. .