玉米细菌性枯萎病和玉米内州萎焉病的酶联免疫—实时荧光PCR检测

浙江农业学报

玉米细菌性枯萎病和玉米内州萎焉病的酶联免疫—实时荧光PCR检测

（1浙江省检验检疫科学技术研究院，浙江杭州 310016；2杭州出入境检验检疫局，浙江杭州 310006）

出版日期:2014-09-25 发布日期:2014-10-11

The immuno\|capture real\|time PCR detection technology of Pantoea.stewartii subsp. stewarii and Clavibacter michiganensis subsp. nebraskense

（1 Zhejiang Academy of Science & Technology for Inspection & Quarantine， Hangzhou 310016， China;2 Hangzhou Entry\|Exit Inspection and Quarantine Bureau， Hangzhou 310006， China）

Online:2014-09-25 Published:2014-10-11

摘要/Abstract

摘要： 针对玉米细菌性枯萎病和玉米内州萎焉病这两种检疫性病菌进行酶联免疫—实时荧光PCR检测研究，对该方法进行了特异性、灵敏度以及实际样品模拟试验。结果表明，该方法具有较好的特异性，检测低限在100～200 cfu·mL-1，同时可用于实际样品的检测鉴定。

关键词: 玉米细菌性枯萎病, 玉米内州萎焉病, 酶联免疫—实时荧光PCR

Abstract: An immuno\|capture PCR （IC\|PCR） method was studied to detect two quarantine bacterial， Pantoea stewartii subsp. stewarii and Clavibacter michiganensis subsp. nebraskense. Specificity and sensitivity of the method were examined， and the actual sample simulation experiment was conducted. The results showed that this method was high species\|specific. The minimum limit of detection is between 10-100 cfu·mL-1. The method can also be used for detection and identification of the entry\|and\|exit samples.

Key words: Pantoea stewartii subsp. stewarii, Clavibacter michiganensis subsp. nebraskense, immuno\, capture PCR

陈曦1，张明哲1，林晓佳1，吴志毅1，陈吴健1，吴蓉2，夏拯1. 玉米细菌性枯萎病和玉米内州萎焉病的酶联免疫—实时荧光PCR检测[J]. 浙江农业学报.