浙江农业学报

• 植物保护 • 上一篇    下一篇

多粘类芽孢杆菌的保藏及复壮方法研究

  

  1. (1 浙江工业大学 生物与环境工程学院,浙江 杭州 310014;2 浙江钱江生物化学股份有限公司,浙江 海宁 314400)
  • 出版日期:2015-01-25 发布日期:2015-01-17

Preservation method and rejuvenation method of Paenibacillus polymyxa

  1. (1 College of Biological and Environmental Engineering, Zhe Jiang University of Technology, Hangzhou 310014, China; 2 Zhe Jiang Qiang Jiang Biochemical Stock Limited Company, Hangzhou 314400, China)
  • Online:2015-01-25 Published:2015-01-17

摘要: 研究了保护剂、保藏及复壮方法对多粘类芽孢杆菌C12菌种存活率和产抗生素能力的影响。结果表明,以20%脱脂奶粉为保护剂,冻藏的存活率是冻干的17.3倍。冻藏中,20%脱脂奶粉添加海藻糖和二甲亚砜后,菌种的抗生素相对效价平均值比不添加的分别提高4.5%和4.1%。冻干中,20%脱脂奶粉添加海藻糖,菌种的抗生素相对效价平均值提高6%,存活率提高5倍。冻藏6个月的菌种经过3次循环分离纯化后,菌株产抗生素能力最高恢复至93%,平均恢复至80%;经抗性平板1次分离纯化复壮,菌株产抗生素能力最高恢复至81%,平均恢复至45%。

关键词: 多粘类芽孢杆菌, 多粘菌素, 菌种保藏, 菌种复壮

Abstract: The effects of protective agent, preservation method and rejuvenation method on Paenibacillus polymyxa C12's survival rate and production ability were studied. The results were as following, using 20% skimmed milk powder as protective agent, the survival rate of the strains in -80℃ preservation was 17.3 times higher than the freeze-drying preservation; In the -80℃ preservation, adding thehalose and dimethyl sulfoxde into 20% skimmed milk, the average relative antibiotic titer increased by 4.5% and 4.1%, respectively; In the freeze-drying preservation, adding thehalose into 20% skimmed milk the average antibiotic production increased by 6.0%, and the survival rate increased by 5 times; The strains preserved in -80℃ for six months, then separated and purified for three times,the highest antibiotic production capacity returned to 93%, and the average antibiotic production capacity returned to 80%; Using resistance plate to separate and purify for one time, the highest antibiotic production capacity returned to 81%, and the average antibiotic production capacity returned to 45%.

Key words: Paenibacillus polymyxa, polymyxin E, preservation, rejuvenation