含抗草甘膦CP4\|EPSPS基因和耐逆Trihelix类转录因子GmGT\|2A基因的植物表达载体构建及转化验证

浙江农业学报

• 作物科学 • 下一篇

含抗草甘膦CP4\|EPSPS基因和耐逆Trihelix类转录因子GmGT\|2A基因的植物表达载体构建及转化验证

（1浙江省农业科学院作物与核技术利用研究所，浙江杭州310021；2浙江师范大学化学与生命科学学院，浙江金华321004；3长江大学园艺园林学院，湖北荆州 434023）

出版日期:2015-02-25 发布日期:2015-03-12

Construction and functional verification of a plant expression vector containing both herbicide resistant gene CP4\|EPSPS and stress tolerance related transcription factor GmGT\|2A

（1 Institute of Crop and Nuclear Technology Utilization， Zhejiang Academy of Agricultural Sciences， Hangzhou 310021， China; 2 College of Chemistry and Life Sciences， Zhejiang Normal University， Jinhua 321004， China;3 College of Horticulture and Garden， Yangtze University， Jingzhou 434023， China）

Online:2015-02-25 Published:2015-03-12

摘要/Abstract

摘要： 高效易用的植物表达载体在基因功能相关研究中起着重要的作用。以植物表达载体pBA002为基础载体，使用抗草甘膦CP4\|EPSPS基因替换原载体的Bar筛选标记基因，获得新的植物表达载体pES002。将大豆耐逆相关Trihelix类转录因子GmGT\|2A连接到pES002，构建了同时含耐逆相关基因和抗除草剂标记基因的植物表达载体pES002\|GmGT\|2A。利用农杆菌介导的花序浸润法转化拟南芥，并对转基因植株进行抗草甘膦和耐盐性鉴定。结果表明，转基因植株在抗草甘膦和耐盐性上均显著强于对照，表明该载体可以用于转基因相关的基因功能研究。

关键词: CP4\, EPSPS；GmGT\, 2A；草甘膦；转录因子；表达载体

Abstract: An efficient and easy\|to\|use plant expression vector plays an important role in the research of gene functions. In this study， the glyphosate resistant gene CP4\|EPSPS was cloned to replace the original selective marker Bar gene of plant expression vector pBA002. The new plant expression vector was named as pES002. A Trihelix transcription factor GmGT\|2A was subsequently ligated into pES002 to form a new plant expression vector, namely pES002\|GmGT\|2A. This vector was transformed into Arabidopsis by floral dipping method to verify the functions of both CP4\|EPSPS and GmGT\|2A genes. Results showed that the transgenic lines greatly improved the tolerance to both glyphosate and high salinity stress， indicating that both the new selective marker gene and the target gene worked well. Thus， this vector was successfully constructed and could be further used in transgenic related research.

Key words: CP4\, EPSPS, GmGT\, 2A, glyphosate, transcription factor, expression vector

李雁杰1，2，朱丹华1，冯小锋3，高莎3，杨清华1，董德坤1，*. 含抗草甘膦CP4\|EPSPS基因和耐逆Trihelix类转录因子GmGT\|2A基因的植物表达载体构建及转化验证[J]. 浙江农业学报.

含抗草甘膦CP4\|EPSPS基因和耐逆Trihelix类转录因子GmGT\|2A基因的植物表达载体构建及转化验证

Construction and functional verification of a plant expression vector containing both herbicide resistant gene CP4\|EPSPS and stress tolerance related transcription factor GmGT\|2A

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