盐含量和高压灭菌对培养基酸碱度和凝固状态的影响

浙江农业学报

盐含量和高压灭菌对培养基酸碱度和凝固状态的影响

（中国农业科学院特产研究所，吉林长春 130112）

出版日期:2015-10-25 发布日期:2015-10-20

Effects of salt strength and autoclaving on pH and solidification of medium#br#

（Institute of Special Wild Economic Animals and Plants， Chinese Academy of Agricultural Sciences， Changchun 130112， China）

Online:2015-10-25 Published:2015-10-20

摘要/Abstract

摘要： 将MS，B5和W培养基配制成有机元素含量不变，无机盐含量分别为100%，75%，50%，25%，0的培养基，分别加入不同体积1 mol·L-1的NaOH溶液，测定高压灭菌前后培养基pH值，记录培养基凝固状态，研究不同盐含量MS，B5和W培养基高压灭菌后pH值与NaOH溶液添加量之间的定量关系，以及盐含量和酸碱度变化对培养基凝固状态的影响。结果表明，高压灭菌后，不同盐含量的培养基pH值与NaOH溶液添加量呈对数关系，培养基pH值降低幅度随盐含量增加而减小，调节培养基pH值所需NaOH溶液添加量随盐含量增加而增加，培养基硬度随NaOH溶液添加量增加而增加。

关键词: 培养基, 盐含量, 高压灭菌, 酸碱度, 凝固状态

Abstract: In the present study， MS， B5 and W media with unchanged organic elements and varied inorganic salt strength of 100%， 75%， 50%， 25% and 0， were selected as materials. With addition of different volumes of 1 mol·L-1 NaOH solution， the pH value of media before and after autoclaving were determined， and solidification state of media were examined to clarify the quantitative relationship between pH value and the added volume of NaOH solution， as well as effects of salt strength on pH value and solidification state of MS， B5 and W media after autoclaving. It was shown that there was a logarithmic quantitative relationship between the added volumes of NaOH solution and pH value of media after autoclaving. The media pH value decreased as salt strength increased， while the volume of NaOH solution needed for pH adjustment increased as salt strength increased. The hardness of media increased as the added volume of NaOH solution increased.

Key words: medium, salt strength, autoclaving, pH value, solidification state

张正海，张悦，李爱民，张亚玉*，陈晓丹. 盐含量和高压灭菌对培养基酸碱度和凝固状态的影响[J]. 浙江农业学报.

ZHANG Zheng\|hai， ZHANG Yue， LI Ai\|min， ZHANG Ya\|yu*， CHEN Xiao\|dan. Effects of salt strength and autoclaving on pH and solidification of medium#br#[J]. .

[1]	沈植国, 孙萌, 丁鑫, 程建明, 陈迪新. 不同培养基组分对5个蜡梅品系花粉萌发和花粉管生长的影响[J]. 浙江农业学报, 2021, 33(2): 278-287.
[2]	郑铭洁, 姜铭北, 章明奎, 严建立, 王道泽. 浙江省新垦耕地土壤熟化指标研究[J]. 浙江农业学报, 2020, 32(10): 1834-1840.
[3]	江林娟, 邹雪, 黄雪丽, 倪苏, 李立芹, 杨世民. 响应面法优化马铃薯茎段高效再生体系[J]. 浙江农业学报, 2018, 30(6): 918-925.
[4]	谢丽丹, 王素英. 响应面法优化螺旋藻培养基[J]. 浙江农业学报, 2017, 29(2): 307-314.
[5]	胡秀荣，黄振东，蒲占湑，杜丹超，陈国庆，鹿连明*. 柑橘内生放线菌CR20发酵培养基的优化[J]. 浙江农业学报, 2015, 27(2): 215-.
[6]	陈兵红1，2，汤伟华1，应俊辉1，李秋萍2，王东明1，潘西瑜1，柴红玲1. 沸石培养基对大羽藓(Thuidium cymbifolium)配子体生长的影响[J]. 浙江农业学报, 2014, 26(4): 948-.
[7]	陈胜军;王艳丽;张震;姜华;柴荣耀;毛雪琴;邱海萍;王教瑜;杜新法;;孙国昌; . 稻粒黑粉菌冬孢子萌发影响因素及其产孢培养基的筛选[J]. , 2011, 23(3): 0-576.
[8]	詹兰兰;活泼;刘左军;徐姚力. 两株优良酸奶发酵菌株的生长特性[J]. , 2011, 23(1): 144-149.
[9]	唐华;刘同军;杨海龙;陈卫;*. 产红色素海洋细菌Loktanella sp．发酵培养基的优化[J]. , 2009, 21(1): 0-34.
[10]	秦艳;李卫芬;雷剑;余东游. 凝结芽孢杆菌发酵条件的优化[J]. , 2008, 20(6): 0-474.
[11]	徐丽红;吴应淼;陈俏彪;叶长文;王钢军. 香菇对培养基中有害重金属的吸收富集规律及临界含量值[J]. , 2007, 19(3): 0-215.
[12]	赵映振;严成其;沈岚;倪建刚;皇甫伟国;黄坚;袁凤杰;钱凯先;吴平. 规模化生产非洲菊盆栽品种雨燕专用培养基配方的研究[J]. , 2003, 15(4): 0-259.