关键词: 红菜薹, 黄芽白, 亚种间杂交, 胚培养

Abstract: By artificial pollination and supplementary pollination in bud stage， hybrids were obtained through reciprocal cross of Brassica campestris L. ssp. Chinenses var. purpurea Bialey and B.campestris ssp. pekinensis in high frequency. Leaf， plant type and flower morphology of hybrids were characterized by intermediate type of their parents. The leaf color of hybrid was darker than B.campestris ssp. pekinensis， more closer to purple color of Brassica campestris L. ssp. Chinenses var. purpurea Bialey. The hybrids showed high heterosis for higher plant height， more branches and more pods than the parents， and they had a good seed setting via cross by themselves because of mature flowers which could produce more than 99% fertile pollens. It also indicated that the female gemmates were fertile in the hybrids. All of the chromosomes paired to 10 bivalents at diakinesis in 95% pollen mother cells. A few of lagged chromosomes were observed at metaphase Ⅱ. The hybrids with heterosis and good seed setting was useful for breeding new type of Brassica campestris.

Key words:  B.campestris L. var. purpurea, B.campestris var. pekinensis, hybridization, embryo culture