产玫瑰红色素粘质沙雷氏菌的分离鉴定及抑菌活性

浙江农业学报

产玫瑰红色素粘质沙雷氏菌的分离鉴定及抑菌活性

（1. 四川农业大学动物医学院动物微生态研究中心，四川成都 611130；2. 动物疫病与人类健康四川省重点实验室，四川成都 611130）

出版日期:2016-02-25 发布日期:2016-03-10

Isolation， identification and antibacterial activities of Serratia marcescens producing rosy pigment

（1. Research Center of Animal Microecology， College of Veterinary Medicine， Sichuan Agricultural University， Chengdu 611130， China; 2. Key Laboratory of Animal Disease and Human Health of Sichuan Province， Chengdu 611130， China）

Online:2016-02-25 Published:2016-03-10

摘要/Abstract

摘要： 旨在分离鉴定产玫瑰红色素菌株，及其分离菌株和色素对病原菌的生物拮抗作用。经形态观察、生理生化试验和16S rRNA序列分析对其进行鉴定，通过控制变量法确定最佳产色素的温度及光照条件，破裂菌体提取色素分析此菌株产色素的量和红色素的性质，采用滤纸片法研究对病原菌的拮抗作用。结果表明：（1）分离细菌Dse\|01菌株最终鉴定为粘质沙雷氏菌（Serratia marcescens）；（2）该菌在25～35 ℃条件下培养色素形成较早且颜色较深，30 ℃温度下产色素最佳，光照对其色素形成时间没有明显影响；（3）30 ℃ 150 r·min-1培养24 h，提取获得色素粗品确定为灵菌红素，产量达（567.90±7.77）mg·L-1；（4）分离菌株的全菌液、上清液、菌体和提取色素对病原性大肠杆菌、沙门氏菌均无拮抗作用，而全菌液、菌体和提取色素对金黄色葡萄球菌具有明显的抑制作用。

关键词: 粘质沙雷氏菌, 灵菌红素, 生物拮抗

Abstract: This experiment was conducted to isolate and identify a microbial strain producing rosy pigment， and to study its antibacterial activity. The microbial strains were identified by morphological characteristics， physiochemical properties and 16S rRNA gene sequence analysis. The optimization of temperature and illumination condition for rosy pigment production was investigated by variable-controlling approach. Ultrasonic fragmentation was used to extract pigment and analyze its yield and absorption spectrum. In eventually， antagonism assay to pathogens also be carried out by filter-paper method. The results showed that: （1） a screened strain Dse-01 was identified as Serratia marcescens; （2） The strain showed significant impact on chromogenesis under the condition of 25-35 ℃， especially at 30 ℃， which was not affected by illumination; （3） The strain was cultured at 30 ℃ for 24 h with a shaking speed of 150 r·min-1 and the extracts was prodigiosin， whose production could reach (567.90±7.77) mg·L-1; （4） the pathogenic Escherichia coli and Salmonella spp. were resistant to four active substance: Serratia marcescens， sediment of Serratia marcescens， supernatant of Serratia marcescens and pigment extraction from Serratia marcescens， whereas， Staphylococcus aureus was sensitive to all of these active products except the supernatant.

Key words: Serratia marcescens, prodigiosin, biological antagonism

董婷1，景波1，2，李伟1，黄晓雅1，钱俏君1，杨慧1，潘康成1，2，*. 产玫瑰红色素粘质沙雷氏菌的分离鉴定及抑菌活性[J]. 浙江农业学报.

DONG Ting1， JING Bo1,2， LI Wei1， HUANG Xiao\|ya1， QIAN Qiao\|jun1， YANG Hui1， PAN Kang\|cheng1，2，*. Isolation， identification and antibacterial activities of Serratia marcescens producing rosy pigment[J]. .