浙江农业学报

• 环境科学 • 上一篇    下一篇

重金属Cr6+耐受细菌的筛选研究

  

  1. (华东理工大学 生物反应器工程国家重点实验室,上海 200237)
  • 出版日期:2016-02-25 发布日期:2016-03-10

Screening of heavy metal Cr6+ resistant strains

  1. (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China)
  • Online:2016-02-25 Published:2016-03-10

摘要: 土壤中重金属Cr6+污染日益严重,其对植物的影响也越来越大,并可通过食物链富集进入人和动物体内,从而危害人畜健康。试验在分别添加0.3和0.5 g·kg-1 Cr6+的土样中进行耐性菌株的筛选,以期为微生物修复重金属污染提供技术支持。结果表明,该研究共获得了9株具有较好Cr6+耐性的菌株。它们均可在含0.5,1.0 mmol·L-1 Cr6+的LB培养基中生长。其中,编号为J3,J5,J8的菌种耐性达到2 mmol·L-1,在含2 mmol·L-1 Cr6+的液体培养基中培养48 h后,培养基中的Cr6+含量分别降低23%,36%,7%。经测定,上述3种菌分别为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)、地衣芽孢杆菌(Bacillus licheniformis)、沙福芽孢杆菌(Bacillus sonorensis)。

关键词: 重金属Cr6+, 重金属耐性, 土壤修复

Abstract: Cr6+ is a hazardous heavy metal element to plant growth, and can endanger human and animal health through food chain enrichment. In the present study, Cr6+ resistant strains were screened from soils added with 0.3, 0.5 g·kg-1 Cr6+, in order to provide technical support for microbial remediation of heavy metal pollution. It was shown that 9 Cr6+ resistant strains were screened, all of which could grow normally in media with 0.5, 1.0 mmol·L-1 Cr6+. Among them, strains named as J3, J5 and J8 were able to grow in media with 2 mmol·L-1 Cr6+. Besides, after 48 h cultivation in media with 2 mmol·L-1 Cr6+, Cr6+ content in media with J3, J5, J8 was reduced by 23%,36%, 7%, respectively. Sequencing analysis showed that J3, J5, J8 were Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus sonorensis, respectively.

Key words: heavy metal Cr6+, heavy metal resistance, soil remediation