Mycotoxins contamination situation and toxicity evaluation of moldy aquatic feed in southern Guangdong of China
（1. College of Food Science and Technology， Guangdong Ocean University / Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety / Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution， Zhanjiang 524088， China; 2. The Quality Supervision and Testing of Zhanjiang， Zhanjiang 524022， China; 3. Shenzhen TotalTest Technology Co. Ltd.， Shenzhen 518012， China）
Abstract：In order to understand the latest mycotoxin contamination situation of aquatic feed and raw materials in Guangdong Province， evaluate toxic effect of moldy aquatic feed， and understand their damaging effects on living organisms， a total of 120 samples were randomly collected from the fish and shrimp feed in the south of Guangdong. Enzyme linked immunosorbent assay （ELISA） was used to detect the contents of mycotoxins including aflatoxin B1 （AFB1）， T2 toxin， deoxynivalenol （DON） and ochratoxin （OTA） in above aquatic feeds， and then each four fish feed and shrimp feed with different mycotoxin pollution levels （standard， mild， moderate and severe） were chosen to evaluate biological toxicity for mouse. The results showed that the mycotoxin contamination rate in aquatic feed was high， coexisting phenomenon of several toxins was relatively common; And T2， DON， AFB1 toxin pollutions were the most common， OTA pollution was lighter， T2 detected concentration was the largest （11 928.00 μg·kg-1）. Mice weight increased first then fell， each organ index fell slightly， especially the spleen and kidney indexes. Lymphocyte， hemoglobin， the large platelet rate were decreased significantly （P<0.05）. Sperm deformity rate and micronucleus rate increased with the increasing dose， and significant differences were found in the blood biochemical indexes （P<0.05）. The study showed that mycotoxin contamination was serious and T2 content was the highest in moldy aquatic feed， which had significant damage on mice spleen， kidney and liver organs. Meanwhile， it also had adverse effects on the hematopoietic and immune systems， and had genetic toxicity at certain level.
王小博1，王雅玲1，*，王润东1，吕鹏莉1，孙力军1，刘晓燕1，施琦2，毕思远3. 我国南粤地区霉变水产饲料真菌毒素污染现状及毒性评价[J]. 浙江农业学报, 2016, 28(6): 951-.
WANG Xiaobo1， WANG Yaling1，*， WANG Rundong1， LYU Pengli1， SUN Lijun1， LIU Xiaoyan1， SHI Qi2， BI Siyuan3. Mycotoxins contamination situation and toxicity evaluation of moldy aquatic feed in southern Guangdong of China. , 2016, 28(6): 951-.