浙江农业学报

• 植物保护 • 上一篇    下一篇

北京地区连作草莓根际土壤中真滑刃线虫的鉴定

  

  1. (1.四川省农业科学院 植物保护研究所,农业部西南作物有害生物综合治理重点实验室,四川 成都 610066; 2.中国农业大学 植物保护学院,昆虫与线虫实验室, 北京 100193; 3.河南工业大学 粮油食品学院,粮食储运国家工程实验室,河南 郑州 450001; 4.青岛农业大学 农学与植物保护学院,山东省旱作农业技术重点实验室,山东 青岛 266109)
  • 出版日期:2016-06-25 发布日期:2016-06-12

Identification of Aphelenchus sp. in continuouscropping strawberry rhizosphere soil from Beijing

  1. (1. Key Laboratory of Integrated Pest Management on Crops in Southwest, Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 2. Laboratory of Entomology and Nematology, College of Plant Protection, China Agricultural University, Beijing 100193, China; 3. National Engineering Laboratory for Grain Storage and Logistics, School of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China; 4. Shandong Provincial Key Laboratory of Dryland Technology, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China)
  • Online:2016-06-25 Published:2016-06-12

摘要: 对北京地区连作草莓土壤中占种群优势地位的真滑刃线虫Aphelenchus sp.,采用传统形态学和分子生物学相结合的手段进行了鉴定。结果表明,文中分离得到的真滑刃线虫,虫体形态与燕麦真滑刃 Aphelenchus avenae Bastian极为相似,且主要形态学测量值基本相符。在形态学鉴定结果的基础上,结合ITSrDNA区域序列扩增结果与GenBank已注册的序列比对分析,确定该线虫为燕麦真滑刃线虫。该线虫的分类鉴定为其生物学及生态学特性的深入研究奠定了基础,但由于该线虫在连作草莓的根际土壤中大量存在,其是否对草莓的生长有不利影响值得进一步探讨。

关键词: 连作草莓, 分类鉴定, 食真菌线虫, 燕麦真滑刃线虫

Abstract: Aphelenchus sp.,a dominant nematode species in Beijing continuouscropping strawberry soil, was identified in present study by morphological and molecular methods. The results showed that its morphology was extremely similar with Aphelenchus avenae Bastian. Besides, the main morphological measurements of Aphelenchus sp. and Aphelenchus avenae Bastian were consistent. On the basis of morphological identification results, combining with ITS rDNA regional sequence amplification results with GenBank registered sequence alignment analysis, it was determined that Aphelenchus sp. was a new strain of Aphelenchus avenae. The identification of Aphelenchus sp. laid a solid foundation for the further study of its biology and ecology characteristics. Since Aphelenchus sp. existed in large numbers in the rhizosphere soil of continuouscropping strawberry, its needed to carry out further research to know whether it have adverse effect on the growth of strawberry or not.

Key words: continuouscropping strawberry, taxonomic identification, fungivorous nematode, Aphelenchus avenae Bastian