禽波氏杆菌HagA蛋白基因的序列分析及抗原表位预测

doi:10.3969/j.issn.1004-1524.2017.10.07

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (10): 1642-1647.DOI: 10.3969/j.issn.1004-1524.2017.10.07

禽波氏杆菌HagA蛋白基因的序列分析及抗原表位预测

袁朋, 朱瑞良, 崔晨晨, 杨萍萍^*

山东农业大学动物科技学院,山东泰安271018

收稿日期:2017-03-23 出版日期:2017-10-20 发布日期:2017-12-05
通讯作者: 杨萍萍,E-mail:ppyang@sdau.edu.cn
作者简介:袁朋 (1985—),男,山东德州人,硕士研究生,实验师,研究方向为预防兽医学。E-mail: yp8511@163.com
基金资助:
国家自然科学基金项目(31602067,31272595);山东省自然科学基金项目(ZR2014CM044);山东省“双一流”奖补资金

Sequencing and B-cell epitopes prediction of Bordetella avium HagA protein

YUAN Peng, ZHU Ruiliang, CUI Chenchen, YANG Pingping^*

College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, China

Received:2017-03-23 Online:2017-10-20 Published:2017-12-05

摘要/Abstract

摘要： 试验旨在对禽波氏杆菌的血凝素HagA蛋白的二级结构与B细胞抗原优势表位进行预测,探讨以血凝素HagA蛋白制作单克隆抗体的可能性。首先对禽波氏杆菌的血凝素hagA基因进行了PCR扩增以及序列测定,利用生物信息学的相关软件与分析方法,对血凝素HagA蛋白的二级结构以及B细胞抗原表位进行分析预测。经综合分析表明,禽波氏杆菌的HagA蛋白氨基酸序列中存在7个B细胞优势抗原表位区域,6个可能的糖基化位点。研究结果为进一步分析禽波氏杆菌HagA蛋白的生物学功能、制备单抗和研制疫苗等奠定了基础。

关键词: 禽波氏杆菌, HagA蛋白, 二级结构, B细胞抗原表位预测

Abstract: This article is carried out to predict the secondary structure of Bordetella avium HagA and the dominant epitope of B cell antigen, in order to discuss the possibility of HagA protein in the production of monoclonal antibodies. In this study, hagA gene of B. avium was amplified, cloned and sequenced. The secondary structure and B cell epitopes of the HagA protein of B. avium were analyzed and predicted subsequently by using bioinformatics software. The results showed that the B. avium HagA protein was supposed to include 7 potential antigen epitopes and 6 potential glycosylated sites. The results provide a theoretical basis for the further study of immune mechanisms, monoclonal antibodies preparation and epitope vaccine design.

Key words: Bordetella avium, HagA, secondary structure, B-cell epitope prediction

中图分类号:

S858.3

袁朋, 朱瑞良, 崔晨晨, 杨萍萍. 禽波氏杆菌HagA蛋白基因的序列分析及抗原表位预测[J]. 浙江农业学报, 2017, 29(10): 1642-1647.

YUAN Peng, ZHU Ruiliang, CUI Chenchen, YANG Pingping. Sequencing and B-cell epitopes prediction of Bordetella avium HagA protein[J]. , 2017, 29(10): 1642-1647.

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禽波氏杆菌HagA蛋白基因的序列分析及抗原表位预测

Sequencing and B-cell epitopes prediction of Bordetella avium HagA protein

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