西南部分地区圈养非人灵长类贾第虫感染情况调查及多位点基因序列分析

doi:10.3969/j.issn.1004-1524.2018.03.02

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (3): 366-370.DOI: 10.3969/j.issn.1004-1524.2018.03.02

西南部分地区圈养非人灵长类贾第虫感染情况调查及多位点基因序列分析

田一男¹, 魏斌¹, 李平², 黄祥明³, 李威¹, 涂蕊¹, 但佳明¹, 袁莉¹, 杨亭玉¹, 彭广能¹, 钟志军^1,*

1.四川农业大学动物医学院,动物疫病与人类健康四川省重点实验室,四川成都 611130;
2.邛崃市农业和林业局,四川邛崃 611530;
3.成都大熊猫繁育研究基地四川省濒危野生动物保护生物学省部共建实验室,四川成都 610081

收稿日期:2017-07-31 出版日期:2018-03-20 发布日期:2018-03-21
通讯作者: *钟志军, E-mail: zhongzhijun488@126.com
作者简介:田一男(1994—),女,四川攀枝花人,硕士,从事兽医临床病理学与分子诊断学研究。E-mail: 610436604@qq.com
基金资助:
国家重点研发计划(2016YFD0501009);成都大熊猫繁育研究基金会项目(CPF2015-4)

Prevalence and multilocus gene sequence analysis of Giardia duodenalis in captive non-human primates in parts of Southwest China

TIAN Yinan¹, WEI Bin¹, LI Ping², HUANG Xiangming³, LI Wei¹, TU Rui¹, DAN Jiaming¹, YUAN Li¹, YANG Tingyu¹, PENG Guangneng¹, ZHONG Zhijun^1,*

1. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Agricultural and Forestry Bureau of Qionglai, Qionglai 611530, China;
3. Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China

Received:2017-07-31 Online:2018-03-20 Published:2018-03-21

摘要/Abstract

摘要： 为了解西南部分地区非人灵长类贾第虫的感染情况及其集聚体和基因亚型分布,收集了西南地区部分动物园、养殖场以及试验动物养殖基地的207份猕猴、长臂猿、金丝猴和食蟹猴新鲜粪便,采用饱和蔗糖漂浮法处理样品,提取DNA,经巢式PCR扩增β-giardin(bg)、tpi和gdh基因,扩增产物经测序后进行分子生物学分析。结果表明:西南部分地区圈养非人灵长类贾第虫感染率为7.73%(16/207),16份阳性样品均为集聚体B(assemblage B)。感染的品种包括猕猴、长臂猿及食蟹猴。长臂猿感染率最高(38.89%),不同品种非人灵长类贾第虫感染率差异极显著(P<0.01)。所有阳性样品均成功扩增出bg、tpi和gdh三个基因的特异性产物。多位点基因序列分析和种系进化树分析结果显示,bg及tpi基因位点多态性变异明显,gdh位点多态性变异较小。本次调查结果表明,西南部分地区非人灵长类所携带的贾第虫具有人兽共患风险。

关键词: 非人灵长类, 贾第虫, bg, tpi, gdh, 感染率

Abstract: To understand the prevalence and molecular characteristics of Giardia duodenalis in captive non-human primates (NHPs) in parts of Southwest China, a total of 207 fresh fecal samples from rhesus macaques, northern white-cheeked gibbons, golden snub-nosed monkeys and crab-eating macaques were obtained from zoos, farms and laboratory in Southwest China. The oocysts in samples were collected by the Sheather's sucrose flotation technique, and their DNA was extracted. These DNA samples were used to amplify β-giardin (bg), tpi and gdh genes by nest PCR for detecting Giardia. The results showed that 7.73% (16/207) of samples were positive for G. duodenalis infection, and 16 positive samples from rhesus macaques, northern white-cheeked gibbons and crab-eating macaques were identified as assemblage B. Northern white-cheeked gibbons had the highest infection rate (38.89%), and the difference in infection rates among the species was significant (P<0.01). All the positive samples were successfully amplified based on bg, tpi and gdh genes, multilocus genotyping showed the high genetic diversity of bg and tpi. Based on a phylogenetic analysis, potentially zoonotic genotypes of Giardia duodenalis were identified in NHPs in southwest China.

Key words: non-human primates, Giardia duodenalis, bg, tpi, gdh, infection rate

中图分类号:

S855.9⁺9

田一男, 魏斌, 李平, 黄祥明, 李威, 涂蕊, 但佳明, 袁莉, 杨亭玉, 彭广能, 钟志军. 西南部分地区圈养非人灵长类贾第虫感染情况调查及多位点基因序列分析[J]. 浙江农业学报, 2018, 30(3): 366-370.

TIAN Yinan, WEI Bin, LI Ping, HUANG Xiangming, LI Wei, TU Rui, DAN Jiaming, YUAN Li, YANG Tingyu, PENG Guangneng, ZHONG Zhijun. Prevalence and multilocus gene sequence analysis of Giardia duodenalis in captive non-human primates in parts of Southwest China[J]. , 2018, 30(3): 366-370.

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西南部分地区圈养非人灵长类贾第虫感染情况调查及多位点基因序列分析

Prevalence and multilocus gene sequence analysis of Giardia duodenalis in captive non-human primates in parts of Southwest China

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